Characterization of green-synthesized zinc oxide nanoparticles and its influence on post-harvest shelf-life of garlic against black mold disease caused by Aspergillus niger

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 13, 2025

Garlic is an important spice crop used for flavoring food and has a long history of use in traditional medicine. However, black mold common fungal disease affecting garlic, which was caused by Aspergillus infection. This significantly impacts both the production quality garlic. Therefore, this study aimed to evaluate antifungal activity novel green-synthesized zinc oxide nanoparticles (ZnO-NPs) against diseases An environmentally friendly green synthesis technique produce ZnO-NPs using zinc-tolerant bacteria Serratia sp. (ZTB24). In present experimental analysis viz. UV-Vis spectroscopy at 380 nm, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential confirmed successful biosynthesis from The poisoned spore germination test revealed activities A. niger under vitro conditions. presence disease-causing fungus through its isolation infected garlic bulbs, it further identified molecular level inter-transcribed sequence (ITS) rDNA sequencing. reduced mycelial growth up 90% 73% 250 μg ml −1 concentration ZnO-NPs. were vivo different concentrations (50, 100, 250, 500 ppm) post-harvest treatment percentage severity assessed after 7 14 days, application ppm exhibited 0% pre-inoculation method, while plant recorded 1.10% days 0.90% post-inoculation compared control group. Hence, synthesized paves way development natural fungicides, offering sustainable renewable alternative chemical methods.

Язык: Английский

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Electrospun Nanofibers with Antibacterial and Antioxidant Activities for Air Purification

ACS Applied Nano Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Язык: Английский

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Exploring the molecular biology of zinc-doped hydroxyapatite nanocomposites as fillers for dental materials: a self-defensive approach targeting bacterial DNA

Materials Research Express, Год журнала: 2024, Номер 11(9), С. 095401 - 095401

Опубликована: Сен. 1, 2024

Abstract Infections during or after the denture filling pose a serious threat to recovery later, and antibiotics are scanty for quick healing. The antibacterial potential of Zn has convinced us fabricate its composites with hydroxyapatite (HA), which distinct biological properties. main aim study is evaluate Zn-doped HA investigate molecular interaction bacterial strains enhance defensive ability against attack. This investigates synthesis zinc-doped nano-powder (Zn-HA) as coating materials dental applications. co-precipitation technique was used in this regard obtain purest form nanoparticles. as-prepared Zn-HA samples were characterized by FTIR spectroscopy assess functional groups between components, x-ray diffraction (XRD) determine percentage crystallinity, SEM explore surface morphology composites, EDX confirm incorporation apatite structure. crystalline size decreases from 99 nm 40 an increase doping Zncl 2 0 2.5 g. With increasing concentration doping, effectiveness increased. Due increased doped Zn, ions effectively rupture membrane destroy DNA. Meanwhile, it shows highest activity Pseudomonas (20.2 ± 0.02), Klebsiella (25.8 0.05), Bacillus (18.3 0.09), S. aureus (24 0.03), E. coli (19.3 0.09) Zn/HA5. It showed that Zinc hydroxyapatite, zinc enhances its-defensive attack various strains, makes material efficacy applications like coating, material, restoratives, inhibiting growth colonies biofilm formation oral cavity. In way, Zn/HA efficient composite restorative enhanced may help mitigate risk infections, promote well health, overall improve life dentine.

Язык: Английский

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Comparative study of silica and silica-decorated ZnO and ag nanocomposites for antimicrobial and photocatalytic applications

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 11, 2025

The increasing threat of multi-resistant infectious agents and environmental toxins has led to a demand for new therapeutic catalytic materials. In this study, C-SiO₂ (crystalline silica), Ag-SiO₂ (silver-silica), ZnO-SiO₂ (zinc oxide-silica) nanocomposites (NCs) were synthesized through green methods characterized by UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM). These silica-based nanomaterials investigated as potential photocatalysts the degradation p-nitroanilines their antibacterial antioxidant properties. XRD analysis showed crystalline sizes 14 nm NPs, 18 Ag-SiO₂, 20 NCs. revealed energy band gaps 4.5 eV 3.23 2.84 ZnO-SiO₂. FTIR confirmed formation SiO₂ in all samples Si–O–Si absorption peak. SEM distinct morphologies: NPs agglomerated granular particles, NCs flattened rods or sheets, roughly spherical. Antimicrobial testing that exhibited 80% 88% antimicrobial activity against Escherichia coli Pseudomonas, with also demonstrating strong Staphylococcus. minimum inhibitory concentration (MIC) was found be 80%, outperforming NPs. Antioxidant assays scavenging efficiencies 45%, 57.8%, 71% C-SiO₂, NCs, respectively, at 30 mg/ml. achieved 75% rate p-nitroanilines, while 45%. This study demonstrates are effective materials disinfection pollutant degradation, offering an eco-friendly solution health challenges.

Язык: Английский

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“Self‐Capped” Carbon Dots with Excellent Anti‐Bacteria Effect and an Extremely Low Cytotoxicity Applied for Hand Sanitizer

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Abstract The increasing challenge of antibiotic resistance necessitates the development novel antibacterial strategies. In this study, a kind self‐capped carbon dots is synthesized from methylene blue (MB) and cetyltrimethylammonium chloride (CTAC), named as MC‐CDs that are specifically designed for enhanced photodynamic activity. Under 660 nm laser irradiation, demonstrate high inactivation rates Escherichia coli (96.83%) Staphylococcus aureus (94.44%) at 14 18 µg mL −1 effectively, disrupting bacterial cell membranes. incorporation zinc cations (Zn 2+ ) doping further enhances potential MC‐CDs, enabling substantial efficacy even in absence light due to improved electrostatic interactions with comparison commercial agents such salicylic acid, p ‐chloro‐ m ‐xylenol, triclosan, Zn@MC‐CDs exhibit superior performance. When formulated into hand sanitizer, maintained over 90% efficacy, displaying excellent stability extremely low cytotoxicity, highlighting their safe effective use personal hygiene products. This study introduces dot promising agent, addressing critical need advanced reliable solutions infection control.

Язык: Английский

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Zinc oxide nanoparticle mediated approaches for wound healing

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 263 - 281

Опубликована: Янв. 1, 2025

Язык: Английский

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Photodynamic antimicrobial nanocellulose-based paper with direct Z-scheme heterojunction for fruit freshkeeping

Chemical Engineering Journal, Год журнала: 2025, Номер 508, С. 161067 - 161067

Опубликована: Фев. 27, 2025

Язык: Английский

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Zinc-Doped Antibacterial Coating as a Single Approach to Unlock Multifunctional and Highly Resistant Titanium Implant Surfaces

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Failures of dental and orthopedic implants due to microbial colonization, corrosion, insufficient osseointegration remain persistent clinical challenges. Current implant surface coatings often lack the mechanical robustness needed for long-term success. Therefore, this study developed zinc (Zn)-doped on titanium via plasma electrolytic oxidation (PEO), achieving 11 at % Zn incorporation primarily as oxide (ZnO). The Zn-doped were composed zinc, calcium, phosphorus, oxygen, displaying moderate roughness (∼1 μm), hydrophilic behavior, high crystallinity with anatase rutile phases. Tribological tests demonstrated over a 50% reduction in mass loss, while electrochemical confirmed significantly enhanced corrosion resistance coating higher open circuit potential values, larger Nyquist plot semicircles, impedance values low frequencies compared controls (p < 0.05). also showed superior antimicrobial efficacy, reducing Streptococcus sanguinis viability, completely inhibiting Escherichia coli growth, biofilm biomass by 60%, which may be related sustained release (∼6 μg/cm2) 7 days. Enhanced bioactivity was evidenced greater protein adsorption, increased hydroxyapatite formation, improved preosteoblastic cell metabolism morphology. Ex vivo analyses stability, without morphological or chemical impairment, during insertion removal from bovine rib bone, stability quotient (ISQ) indicating benefits poor bone quality. These findings highlight significant promise advancing technology, offering longevity, defense, optimize outcomes.

Язык: Английский

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A Targeted Core–Shell ZIF-8/Au@Fe₃O₄ Platform with Multiple Antibacterial Pathways for Infected Skin Wound Regeneration

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Bacterial infections seriously retard skin wound healing. To enhance the antibacterial efficiency and subsequent regeneration, a core-shell structured therapeutic platform, named FZAM, was designed with multiple antimicrobial pathways. FZAM consists of nanosized Fe3O4 as core ZIF-8 loaded Au nanoparticles (NPs) maltodextrin shell. NPs form heterojunction that generates hyperthermia abundant reactive oxide species (ROS) under near-infrared (NIR) irradiation. This also exhibits outstanding peroxidase-like activity. When bacteria invade, plays targeting effect to increase interaction between bacteria, synergistic action NIR-induced ROS well Zn2+ from ZIF-8, kills more than 99% at 200 μg mL-1. Fortunately, is cytocompatible even promotes biofunctions fibroblasts endothelial cells. In infected models, sterilizes NIR irradiation subsequently reduces inflammatory response accelerates regeneration. work provides therapy platform for treating infection assistance helping

Язык: Английский

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Cascade Nanozyme-Loaded Sprayable Hydrogels for Fibroblast Rejuvenation and Diabetic Wound Regeneration

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 29, 2025

Multifunctional composite wound dressings hold significant promise for diabetic healing. However, the detrimental role of advanced glycation end-products (AGEs)-reactive oxygen species (ROS) cycle in impeding repair remains underexplored. To disrupt this pathological cycle, zeolitic imidazolate framework-8 (ZIF-8) encapsulated cerium dioxide (CeO2) and adsorbed glucose oxidase (GOx) nanozyme particles ((ZIF-8@CeO2)@GOx, zcg) were loaded into a methacrylic anhydride-modified gelatin (GelMA) hydrogel to form sprayable dressing, zcg/GelMA (zcgG). Physicochemical characterization revealed that GOx catalyzes oxidation, triggering ZIF-8 acid-mediated decomposition release zinc ions CeO2 nanoparticles, thereby enabling cascade depletion, antioxidant, antiglycation functions. In vitro antimicrobial cytotoxicity experiments optimized zcg concentration GelMA. Under oxidative hyperglycemic culture conditions, we validated mechanism blocking AGEs-ROS restoring fibroblast mitochondrial membrane potential, subsequently suppressing cellular senescence. bacterial-infected rat skin model, zcgG group demonstrated substantially reduced inflammatory levels, 68% decrease AGEs, 1.9-fold increase collagen deposition compared blank controls. Within 2 weeks, achieved complete closure, while control retained 28% initial area. This work provides preliminary evidence feasibility using nanozymes break promote

Язык: Английский

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