S-propargyl-cysteine attenuates temporomandibular joint osteoarthritis by regulating macrophage polarization via Inhibition of JAK/STAT signaling
Molecular Medicine,
Год журнала:
2025,
Номер
31(1)
Опубликована: Апрель 7, 2025
Abstract
Background
Temporomandibular
joint
osteoarthritis
(TMJ-OA)
is
a
disease
characterized
by
cartilage
degradation
and
synovial
inflammation,
with
limited
effective
treatment
currently.
Synovial
macrophage
polarization
pivotal
in
TMJ-OA
progression,
making
it
promising
therapeutic
aspect.
This
study
investigated
the
effects
of
S-propargyl-cysteine
(SPRC),
an
endogenous
H2S
donor,
on
its
potential
alleviating
TMJ-OA.
Methods
A
MIA-induced
rat
model
LPS-stimulated
RAW264.7
macrophages
were
employed
to
evaluate
SPRC
vivo
vitro.
TMJ
bone
analyzed
via
micro-CT
histological
methods,
while
markers
expression
assessed
RT-qPCR,
western
blot,
immunofluorescence.
RNA
sequencing
was
performed
macrophages,
JAK2/STAT3
signaling
pathway
validated
using
JAK2-specific
inhibitor
AG490.
The
direct
primary
condylar
chondrocytes
examined
evaluating
ECM
synthesis
degradation.
Co-culture
experiments
further
macrophage-chondrocyte
interactions.
Results
significantly
alleviated
damage
model,
as
demonstrated
improved
volume
structure.
reduced
pro-inflammatory
M1
infiltration
enhanced
anti-inflammatory
M2
polarization.
effectively
inhibited
JAK2/STAT3,
leading
reduction
inflammatory
markers,
including
TNF-α,
IL-6,
iNOS.
revealed
that
SPRC-treated
macrophage-conditioned
medium
chondrocyte
metabolic
activity
restored
integrity.
Conclusions
SPRC-modulated
alleviates
JAK/STAT
downregulation,
thereby
reducing
inflammation
These
findings
position
candidate
for
provide
insights
into
novel
strategies
targeting
synovium-cartilage
crosstalk.
Graphical
abstract
Язык: Английский
Effects of poly(amidoamine) as an extrafibrillar demineralization agent on dentin bonding durability of deciduous teeth
Journal of Dentistry,
Год журнала:
2025,
Номер
unknown, С. 105609 - 105609
Опубликована: Фев. 1, 2025
Язык: Английский
Clearance of Cell-Free DNA: A Novel Target for Therapeutic Utilization in Multiple Systemic Disorders
ACS Biomaterials Science & Engineering,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 3, 2025
Cell-free
DNA
(cfDNA)
holds
significant
promise
for
diagnostic
and
therapeutic
advancements
in
medicine.
This
review
delineates
the
utility
of
cfDNA
diagnostics
its
potential
through
clearance
mechanisms
an
array
diseases.
Damage-associated
molecular
patterns
(DAMPs)
are
endogenous
molecules
released
by
host
cells
during
stress,
or
injury.
As
a
trigger
inflammatory
responses
via
damage-associated
(DAMPs),
cfDNA's
removal
nanotechnological
approaches
can
attenuate
inflammation
promote
tissue
repair.
While
application
is
particularly
auspicious
cancer,
sepsis,
conditions,
it
confronted
with
challenges
including
toxicity,
specificity,
rigors
clinical
trial
validation.
Collectively,
this
novel
targets
to
inform
development
innovative
treatment
strategies.
Язык: Английский
Synthesis and application of poly(amidoamine) functional biomedical materials in the field of biomedicine over the past decade
Journal of the Chinese Chemical Society,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 28, 2024
Abstract
Over
the
past
decade,
significant
advancements
have
been
achieved
in
field
of
biomedicine,
particularly
treatment
critical
illness.
However,
there
remain
numerous
challenges
and
barriers
persist
drug
delivery,
medical
imaging,
diagnosis,
treatment.
Poly(amidoamine)
(PAMAM)
dendrimers
emerged
as
promising
candidates
for
development
functional
biomedical
materials
due
to
their
advantageous
properties,
including
ease
preparation,
controllable
size,
abundant
cavities,
water
solubility,
modifiability,
biocompatibility.
This
article
presents
a
comprehensive
review
synthesis,
application,
potential
toxicity
PAMAM
materials,
exploring
role,
significance,
applications
detail.
The
aim
is
provide
valuable
reference
future
inspire
further
discoveries
polyamide
materials.
Язык: Английский