Polylactic-Co-Glycolic Acid/Alginate/Neem Oil-Reduced Graphene Oxide as a pH-Sensitive Nanocarrier for Hesperidin Drug Delivery: Antimicrobial and Acute Otitis Media Assessments
Pharmaceuticals,
Год журнала:
2025,
Номер
18(3), С. 381 - 381
Опубликована: Март 7, 2025
Background/Objectives:
Hesperidin
(HSP)
is
a
potent
phytochemical
antioxidant
and
anti-inflammatory
agent
that
protects
against
otitis
media.
However,
due
to
its
low
solubility
bioavailability,
suitable
delivery
method
needed
overcome
these
problems.
A
hydrogel
promising
nanocarrier
for
controlled
drug
in
response
external
stimuli,
such
as
pH
variations.
Methods:
Graphene
oxide
(GO)-based
nanocarriers
encapsulate
hesperidin
were
further
coated
with
polylactic-co-glycolic
acid/alginate
(PLGA-Alg)
before
being
integrated
into
green
neem
oil
(N.O.)
double
emulsion
produce
synergistic
effect
then
characterized
by
different
assays.
Results:
The
exhibited
substantial
particle
size
(168
±
0.32
nm),
high
encapsulation
(89.86
0.23%)
zeta
potential
of
37
0.43
mV.
In
vitro
release
studies
conducted
over
96
h
indicated
sustained
HSP
82%
at
5.4
65%
7.4.
GO-HSP-loaded
formulation
exhibits
antibacterial
activity,
evidenced
inhibition
zones
39
0.02
mm
Staphylococcus
epidermidis,
considerable
antifungal
activity
Candida
albicans,
an
zone
43
0.13
mm,
along
biofilm
activity.
demonstrated
(5.21
µg/mL)
increased
cell
viability
(90–95%)
while
maintaining
cytotoxicity
HSE-2
cells.
histopathological
analysis
confirmed
treatment
the
reduced
levels
pro-inflammatory
cytokines
(IL-1β,
TNF-α,
TLR4,
IL-6)
raised
markers
(Nrf-2,
SOD)
vivo
rat
model
Conclusions:
GO-based
PLGA-Alg
deliver
enhanced
antibacterial,
antifungal,
properties.
This
may
be
used
treat
media
other
oxidative
stress
diseases.
Язык: Английский
An exploration of the ocular mysteries linking nanoparticles to the patho-therapeutic effects against keratitis
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 6, 2025
Microbial
keratitis,
a
sight-threatening
corneal
infection,
remains
significant
global
health
concern.
Conventional
therapies
using
antimicrobial
agents
often
suffers
from
limitations
such
as
poor
drug
penetration,
side
effects,
and
occurrence
of
resistance,
with
prognosis.
Novel
treatment
techniques,
their
unique
properties
targeted
delivery
capabilities,
offers
promising
solution
to
overcome
these
challenges.
This
review
delves
into
timely
update
the
state-of-the-art
advance
therapeutics
for
keratitis
treatment.
The
diverse
microbial
origins
including
viral,
bacterial,
fungal
infections,
exploring
complex
pathogenic
mechanisms,
followed
by
resistance
mechanisms
in
pathogens
are
reviewed
briefly.
Importantly,
emerging
therapeutic
techniques
piezodynamic
therapy,
photothermal
photodynamic
nanoenzyme
metal
ion
therapy
summarized
this
showcasing
potential
traditional
treatments.
challenges
future
directions
nanotechnology-based
approaches
discussed,
focusing
on
safety,
targeting
strategies,
combination
therapies.
aims
inspire
researchers
revolutionize
accelerate
development
functional
materials
different
Язык: Английский
Chitosan-Based Intelligent Microneedles for Delivery of Amphotericin B Loaded Oleosomes: Antifungal Ocular Patch Targeting for Effective Against Fungal Keratitis Using Rabbit Model via TLR4/NLRP3 Pathway
International Journal of Nanomedicine,
Год журнала:
2025,
Номер
Volume 20, С. 5949 - 5981
Опубликована: Май 1, 2025
Fungal
keratitis
(FK),
a
major
cause
of
blindness,
remains
challenging
to
treat
due
poor
drug
penetration
and
antifungal
resistance.
Amphotericin-B
(AmB),
water-insoluble
low-permeability,
necessitates
innovative
delivery
systems
improve
its
therapeutic
efficacy.
AmB
was
encapsulated
within
oleosomes
(Ole)
prepared
using
the
ethanol
injection
method,
phosphatidylcholine
(Lipoid
S100)
sodium
oleate,
resulting
in
nanosized
spherical
globules.
The
optimized
Ole
were
characterized,
then
selected
incorporated
into
polyacrylate/PEG/chitosan-based
microneedles
(AmB-Ole/MNs)
ocular
by
creating
transient
microchannels
on
eye
surface.
showed
droplet
size
(175
±
0.78
nm),
polydispersity
index
(0.33
0.04),
zeta
potential
(31
0.43
mV),
high
entrapment
efficiency
(91±0.63%),
improved
stability,
bioavailability,
controlled
release.
AmB-Ole/MNs
system
increases
corneal
contact
time
via
surface,
achieving
sustained
over
72
hours
with
70%
ex
vivo
permeation
80
compared
AmB.
In
vitro
activity
histopathological
examination
that
has
potent
biofilm
disruption
(>90%)
27
mm
32
zones
inhibition
against
Candida
albicans
Aspergillus
niger,
respectively.
Cytotoxicity
test
reduced
toxicity
biocompatibility
rabbit
model,
tolerance
targeting
TLR4/NLRP3
pathways
studies.
as
an
platform
for
fungal
offers
release,
enhanced
permeation,
activity,
toxicity.
promise
FK.
Язык: Английский