Polylactic-Co-Glycolic Acid/Alginate/Neem Oil-Reduced Graphene Oxide as a pH-Sensitive Nanocarrier for Hesperidin Drug Delivery: Antimicrobial and Acute Otitis Media Assessments DOI Creative Commons
Saeed A. S. Al-Zuhairy, Sammar Fathy Elhabal, Mohamed Fathi Mohamed Elrefai

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(3), С. 381 - 381

Опубликована: Март 7, 2025

Background/Objectives: Hesperidin (HSP) is a potent phytochemical antioxidant and anti-inflammatory agent that protects against otitis media. However, due to its low solubility bioavailability, suitable delivery method needed overcome these problems. A hydrogel promising nanocarrier for controlled drug in response external stimuli, such as pH variations. Methods: Graphene oxide (GO)-based nanocarriers encapsulate hesperidin were further coated with polylactic-co-glycolic acid/alginate (PLGA-Alg) before being integrated into green neem oil (N.O.) double emulsion produce synergistic effect then characterized by different assays. Results: The exhibited substantial particle size (168 ± 0.32 nm), high encapsulation (89.86 0.23%) zeta potential of 37 0.43 mV. In vitro release studies conducted over 96 h indicated sustained HSP 82% at 5.4 65% 7.4. GO-HSP-loaded formulation exhibits antibacterial activity, evidenced inhibition zones 39 0.02 mm Staphylococcus epidermidis, considerable antifungal activity Candida albicans, an zone 43 0.13 mm, along biofilm activity. demonstrated (5.21 µg/mL) increased cell viability (90–95%) while maintaining cytotoxicity HSE-2 cells. histopathological analysis confirmed treatment the reduced levels pro-inflammatory cytokines (IL-1β, TNF-α, TLR4, IL-6) raised markers (Nrf-2, SOD) vivo rat model Conclusions: GO-based PLGA-Alg deliver enhanced antibacterial, antifungal, properties. This may be used treat media other oxidative stress diseases.

Язык: Английский

An exploration of the ocular mysteries linking nanoparticles to the patho-therapeutic effects against keratitis DOI Creative Commons

Siying Qu,

Shuihua Zheng,

Saleh Muhammad

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 6, 2025

Microbial keratitis, a sight-threatening corneal infection, remains significant global health concern. Conventional therapies using antimicrobial agents often suffers from limitations such as poor drug penetration, side effects, and occurrence of resistance, with prognosis. Novel treatment techniques, their unique properties targeted delivery capabilities, offers promising solution to overcome these challenges. This review delves into timely update the state-of-the-art advance therapeutics for keratitis treatment. The diverse microbial origins including viral, bacterial, fungal infections, exploring complex pathogenic mechanisms, followed by resistance mechanisms in pathogens are reviewed briefly. Importantly, emerging therapeutic techniques piezodynamic therapy, photothermal photodynamic nanoenzyme metal ion therapy summarized this showcasing potential traditional treatments. challenges future directions nanotechnology-based approaches discussed, focusing on safety, targeting strategies, combination therapies. aims inspire researchers revolutionize accelerate development functional materials different

Язык: Английский

Процитировано

0
Polylactic-Co-Glycolic Acid/Alginate/Neem Oil-Reduced Graphene Oxide as a pH-Sensitive Nanocarrier for Hesperidin Drug Delivery: Antimicrobial and Acute Otitis Media Assessments DOI Creative Commons
Saeed A. S. Al-Zuhairy, Sammar Fathy Elhabal, Mohamed Fathi Mohamed Elrefai

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(3), С. 381 - 381

Опубликована: Март 7, 2025

Background/Objectives: Hesperidin (HSP) is a potent phytochemical antioxidant and anti-inflammatory agent that protects against otitis media. However, due to its low solubility bioavailability, suitable delivery method needed overcome these problems. A hydrogel promising nanocarrier for controlled drug in response external stimuli, such as pH variations. Methods: Graphene oxide (GO)-based nanocarriers encapsulate hesperidin were further coated with polylactic-co-glycolic acid/alginate (PLGA-Alg) before being integrated into green neem oil (N.O.) double emulsion produce synergistic effect then characterized by different assays. Results: The exhibited substantial particle size (168 ± 0.32 nm), high encapsulation (89.86 0.23%) zeta potential of 37 0.43 mV. In vitro release studies conducted over 96 h indicated sustained HSP 82% at 5.4 65% 7.4. GO-HSP-loaded formulation exhibits antibacterial activity, evidenced inhibition zones 39 0.02 mm Staphylococcus epidermidis, considerable antifungal activity Candida albicans, an zone 43 0.13 mm, along biofilm activity. demonstrated (5.21 µg/mL) increased cell viability (90–95%) while maintaining cytotoxicity HSE-2 cells. histopathological analysis confirmed treatment the reduced levels pro-inflammatory cytokines (IL-1β, TNF-α, TLR4, IL-6) raised markers (Nrf-2, SOD) vivo rat model Conclusions: GO-based PLGA-Alg deliver enhanced antibacterial, antifungal, properties. This may be used treat media other oxidative stress diseases.

Язык: Английский

Процитировано

0