Amphoteric Cross-Linked Cellulose Nanoparticles as a Platform for Immobilization of Proteins and Cells, Enabling Bioanalyte Sensing DOI

Surya Dev Mishra,

Jyotsna Kawadkar,

Pradyumna Ajit Joshi

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

Cellulosic nanomaterials have significantly promoted the development of sensing devices, drug delivery, and bioreactor processes. Their synthetic flexibility makes them a prominent choice for immobilizing biomolecules or cells. In this work, we developed practical user-friendly approach to accessing cellulose nanoparticles (CNPs). The route is convenient does not require separate purification protocol. These particles are extensively characterized with FTIR, PXRD, TGA, DLS, SEM. Later, functionalized two chemically orthogonal handles: hydroxylamine aldehyde. While prior engaged glycan on bacterial surface, latter could capture an antibiotic promote in vitro controlled release. Besides, their dense functionalization enables efficient inter-CNP reactions, resulting amphoteric covalent cross-linked CNP capable proteins Also, it dual immobilization offer proximity control. Its capabilities were validated by installing aldehyde-equipped bacterium activable fluorophore platform detecting H2S, secretory reductant. It conveniently extends H2S detection chicken eggs. Overall, probe-, enzyme-, cell-equipped offers potential support bioprocesses producing enzymes, secondary metabolites, vitamins, hormones.

Язык: Английский

Amphoteric Cross-Linked Cellulose Nanoparticles as a Platform for Immobilization of Proteins and Cells, Enabling Bioanalyte Sensing DOI

Surya Dev Mishra,

Jyotsna Kawadkar,

Pradyumna Ajit Joshi

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

Cellulosic nanomaterials have significantly promoted the development of sensing devices, drug delivery, and bioreactor processes. Their synthetic flexibility makes them a prominent choice for immobilizing biomolecules or cells. In this work, we developed practical user-friendly approach to accessing cellulose nanoparticles (CNPs). The route is convenient does not require separate purification protocol. These particles are extensively characterized with FTIR, PXRD, TGA, DLS, SEM. Later, functionalized two chemically orthogonal handles: hydroxylamine aldehyde. While prior engaged glycan on bacterial surface, latter could capture an antibiotic promote in vitro controlled release. Besides, their dense functionalization enables efficient inter-CNP reactions, resulting amphoteric covalent cross-linked CNP capable proteins Also, it dual immobilization offer proximity control. Its capabilities were validated by installing aldehyde-equipped bacterium activable fluorophore platform detecting H2S, secretory reductant. It conveniently extends H2S detection chicken eggs. Overall, probe-, enzyme-, cell-equipped offers potential support bioprocesses producing enzymes, secondary metabolites, vitamins, hormones.

Язык: Английский

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