Current Understanding and Translational Prospects of Tetrahedral Framework Nucleic Acids
JACS Au,
Год журнала:
2025,
Номер
5(2), С. 486 - 520
Опубликована: Фев. 10, 2025
Tetrahedral
framework
nucleic
acids
(tFNAs)
represent
a
promising
advancement
in
acid
nanotechnology
due
to
their
unique
structural
properties,
high
biocompatibility,
and
multifaceted
biomedical
applications.
Constructed
through
one-pot
annealing
method,
four
single-stranded
DNAs
self-assemble
into
stable,
three-dimensional
tetrahedral
nanostructures
with
enhanced
mechanical
robustness
physiological
stability,
resisting
enzymatic
degradation.
Their
ability
permeate
mammalian
cells
without
transfection
agents,
coupled
modifiable
surfaces,
positions
tFNAs
as
versatile
carriers
for
drug
gene
delivery
systems.
The
tFNA-based
platforms
exhibit
superior
therapeutic
efficacy,
including
antioxidative
anti-inflammatory
effects,
alongside
efficient
cellular
uptake
tissue
penetration.
These
features
underpin
role
precision
medicine,
enabling
targeted
of
diverse
agents
such
synthetic
compounds,
peptides,
acids.
Additionally,
demonstrate
significant
potential
regenerative
immune
modulation,
antibacterial
strategies,
oncology.
By
addressing
challenges
translational
integration,
stand
poised
accelerate
the
development
research
clinical
applications,
fostering
novel
therapies
enhancing
outcomes
across
wide
spectrum
diseases.
This
Perspective
thoroughly
details
attributes
applications
critically
evaluates
tFNAs'
potential,
outlining
inherent
implementation
exploring
solutions
these
obstacles.
Язык: Английский
A Noninvasive Nanoeyedrop Therapy for the Inhibition of Uveal Melanoma: Tetrahedral Framework Nucleic Acid–Based Bioswitchable MicroRNA Delivery System
ACS Nano,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 10, 2025
Uveal
melanoma
(UM)
is
the
most
prevalent
primary
intraocular
malignancy,
exhibiting
pronounced
invasive
characteristics
and
a
dismal
prognosis.
Conventional
therapeutic
modalities,
including
radiotherapy,
laser
therapy,
surgery,
are
frequently
can
lead
to
complications,
underscoring
need
for
development
of
efficacious,
safe,
noninvasive
approaches.
This
study
investigated
tetrahedral
framework
nucleic
acid
(tFNA)-based
bioswitchable
microRNA
(miRNA)
delivery
system,
designated
BiRDS,
engineered
inhibition
UM
through
use
miRNA
suppressors
via
eyedrops.
The
BiRDS
construct
exhibited
structure,
which
was
small
in
size,
easily
synthesizable,
stable,
biosafe,
able
efficiently
carry
miR-30a-5p
into
cells.
Functionally,
observed
inhibit
proliferation,
migration,
invasion
cells
while
promoting
apoptosis
miR-30a-5p/E2F7
axis.
It
noteworthy
that
nanoeyedrops
were
penetrate
complex
ocular
barrier
structure
reach
fundus,
thereby
inhibiting
growth
xenograft
model.
As
patient-friendly,
eyedrop-based
not
only
inhibited
without
enucleation
eyeball
but
also
expected
improve
patient
compliance
quality
life
providing
safer
alternative
drug
administration.
work
substantiates
as
potential
paradigm
shift
local
treatment
early
UM,
facilitating
its
application
treating
other
diseases
therapies.
Язык: Английский