Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide DOI Creative Commons
Yaşar Gök, Deniz Göl,

Betül Zehra Temur

и другие.

Biomolecules, Год журнала: 2025, Номер 15(6), С. 767 - 767

Опубликована: Май 27, 2025

Polymer coatings as thin films stand out a commonly used strategy to modify biosensor surfaces for improving detection performance; however, nonspecific biomolecule interactions and the limited degree of ligand conjugation on surface have necessitated development innovative methods modification. To this end, methacrylated tethered telechelic polyethylene glycol (PEG-diMA) chains three different molecular weights (2, 6, 10 kDa) were synthesized herein obtaining thiolated nanoparticles (NPs) upon adding excess amounts tetra-thiol crosslinker. Characterized according their size, charge, morphology, thiol amounts, these immobilized gold that mimicked gold-coated mass sensor platforms. The PEG-based nanoparticles, prepared especially by PEG6K-diMA polymers, shown result in preparation monolayer smooth coating 80–120 nm thickness. Cysteine-modified NTS(8–13) peptide (RRPYIL) was conjugated NP with reversible disulfide bonds it demonstrated its cleavage reducing agent such dithiothreitol (DTT) restores NP-immobilized at least two cycles. Together binding studies NTSR2 antibodies, revealed peptide-conjugated NP-modified could be employed model reusable biomarkers same or types.

Язык: Английский

Nanoparticle-Based Biosensors for Salivary Analysis in Dentistry DOI
Ali Doğan, Soley Arslan,

Mustafa Nisair

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

We have focused on nanoparticles(NPs)-based biosensors to detect various biomarkers, including pathogens in saliva, for the determination of disease activities dentistry. Recently, NPs-based studies related dentistry research been receiving growing interest from researchers. The NPs potential give rapid and efficient responses detection targets owing their unique enhanced physico-chemical biological properties. discussed consisting plasmonic (silver, gold NPs, etc.), metallic (quantum dots, carbon family (graphene oxide, single-walled nanotubes, multi-walled hybrid NPs.

Язык: Английский

Процитировано

0
Machine learning assisted nanozyme sensor array for accurate identification and discrimination of flavonoids in healthy tea DOI
Zemin Ren, Qingxu Deng, Yu Wang

и другие.

Food Chemistry, Год журнала: 2025, Номер 486, С. 144612 - 144612

Опубликована: Май 2, 2025

Язык: Английский

Процитировано

0
Mechanism of Cationic Peptide‐Induced Assembly of Gold Nanoparticles: Modulation of Electrostatic Repulsion DOI Creative Commons
Benjamin Lam, Robert Ramji, Margaret Mullooly

и другие.

Aggregate, Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

ABSTRACT The aggregation of plasmonic nanoparticles can lead to new and controllable properties useful for numerous applications. We recently showed the reversible gold (AuNPs) via a small, cationic di‐arginine peptide; however, mechanism underlying this is not yet comprehensively understood. Here, we seek insights into intermolecular interactions peptide‐induced assembly citrate‐capped AuNPs by empirically measuring how peptide identity impacts AuNP aggregation. examined nanoscale between peptides UV‐vis spectroscopy determine structure‐function relationship length charge on Careful tuning sequence demonstrated that driven reduction in electrostatic repulsion. show acetylated N‐terminals carboxylic acid C‐terminals decrease effectiveness inducing increase size through addition glycine or proline units hinders leads less redshift. Arginine‐based were also found be more effective assembling than cysteine‐based equivalent length. illustrate independent stereochemistry. Finally, demonstrate modulation peptide‐AuNP behavior changes pH, salt concentration, temperature. Notably, histidine‐based tyrosine‐based could reversibly aggregate response pH.

Язык: Английский

Процитировано

0
Preparation of Nanoparticle-Immobilized Gold Surfaces for the Reversible Conjugation of Neurotensin Peptide DOI Creative Commons
Yaşar Gök, Deniz Göl,

Betül Zehra Temur

и другие.

Biomolecules, Год журнала: 2025, Номер 15(6), С. 767 - 767

Опубликована: Май 27, 2025

Polymer coatings as thin films stand out a commonly used strategy to modify biosensor surfaces for improving detection performance; however, nonspecific biomolecule interactions and the limited degree of ligand conjugation on surface have necessitated development innovative methods modification. To this end, methacrylated tethered telechelic polyethylene glycol (PEG-diMA) chains three different molecular weights (2, 6, 10 kDa) were synthesized herein obtaining thiolated nanoparticles (NPs) upon adding excess amounts tetra-thiol crosslinker. Characterized according their size, charge, morphology, thiol amounts, these immobilized gold that mimicked gold-coated mass sensor platforms. The PEG-based nanoparticles, prepared especially by PEG6K-diMA polymers, shown result in preparation monolayer smooth coating 80–120 nm thickness. Cysteine-modified NTS(8–13) peptide (RRPYIL) was conjugated NP with reversible disulfide bonds it demonstrated its cleavage reducing agent such dithiothreitol (DTT) restores NP-immobilized at least two cycles. Together binding studies NTSR2 antibodies, revealed peptide-conjugated NP-modified could be employed model reusable biomarkers same or types.

Язык: Английский

Процитировано

0