Natural, safety immunomodulatory derivatives of lactobacillus biofilms promote diabetic wound healing by metabolically regulating macrophage phenotype and alleviating local inflammation DOI Creative Commons

Qingwei Zhou,

Junjie Chi, Jintao Yang

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Long-term inflammatory microenvironment further impairs the healing process of diabetic wounds. Many studies have shown that Lactobacillus can regulate immune function and promote injured tissue repair. However, immunomodulatory safety biofilm (LB) on wounds need investigation. In this present research, we proposed a "bacteria-free derivative therapy" successfully extracted derivatives (LBDs) by ultrasonic separation filtration technology for natural safe treatment The study first cultured anaerobically LBDs using ultrasound combined with technology. were characterized via scanning electron microscopy, Concanavalin A fluorescence staining, protein gel electrophoresis. vivo wound model, closure rates dynamically monitored, sections analyzed hematoxylin-eosin immunofluorescence staining to evaluate LBDs' effects. An in vitro macrophage inflammation model was established, employing immunofluorescence, flow cytometry, Western blotting techniques explore molecular mechanisms underlying effects phenotypes. Furthermore, whole-genome sequencing proteomics LBDs-treated macrophages performed elucidate intrinsic through which effectively utilizing coupled Studies revealed modulate systemic metabolic reprogramming wound-site macrophages, suppress JAK-STAT1 signaling pathway, alleviate local microenvironment, neovascularization ultimately accelerate healing. retains most bioactive components LB. As natural, agent, metabolically phenotypes improving offering promising potential regenerative applications management.

Язык: Английский

Gum arabic based multifunctional antibacterial adhesion hydrogel dressings loaded with doxycycline hydrochloride for wound healing DOI

Yan Ding,

Qida Zong,

Qianwen Zhang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141284 - 141284

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Natural, safety immunomodulatory derivatives of lactobacillus biofilms promote diabetic wound healing by metabolically regulating macrophage phenotype and alleviating local inflammation DOI Creative Commons

Qingwei Zhou,

Junjie Chi, Jintao Yang

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Long-term inflammatory microenvironment further impairs the healing process of diabetic wounds. Many studies have shown that Lactobacillus can regulate immune function and promote injured tissue repair. However, immunomodulatory safety biofilm (LB) on wounds need investigation. In this present research, we proposed a "bacteria-free derivative therapy" successfully extracted derivatives (LBDs) by ultrasonic separation filtration technology for natural safe treatment The study first cultured anaerobically LBDs using ultrasound combined with technology. were characterized via scanning electron microscopy, Concanavalin A fluorescence staining, protein gel electrophoresis. vivo wound model, closure rates dynamically monitored, sections analyzed hematoxylin-eosin immunofluorescence staining to evaluate LBDs' effects. An in vitro macrophage inflammation model was established, employing immunofluorescence, flow cytometry, Western blotting techniques explore molecular mechanisms underlying effects phenotypes. Furthermore, whole-genome sequencing proteomics LBDs-treated macrophages performed elucidate intrinsic through which effectively utilizing coupled Studies revealed modulate systemic metabolic reprogramming wound-site macrophages, suppress JAK-STAT1 signaling pathway, alleviate local microenvironment, neovascularization ultimately accelerate healing. retains most bioactive components LB. As natural, agent, metabolically phenotypes improving offering promising potential regenerative applications management.

Язык: Английский

Процитировано

0