Magnetic Hierarchically Mesoporous UIO-66(Ce/Ti) Core-Shell Nanocomposites for Efficient Enrichment of Phosphopeptides
Abstract
Hierarchically
mesoporous
metal-organic
frameworks
(HMUIO-66(Ce/Ti))
was
designed
and
fabricated
firstly
by
using
a
relatively
rigid
hydroxypropyl
methylcellulose
(HPMC)
coupled
with
poly(ethylene
oxide-b-propylene
oxide-b-ethylene
oxide,
P123)
as
soft
template.
By
introducing
the
synergistic
effect
of
Hofmeister
ions
template
HPMC
P123
into
water-rich
micelle
system,
obtained
HMUIO-66(Ce/Ti)
possessed
irregularly
architecture
wide
pore
size
distribution
8.4
~
26.8
nm.
Furthermore,
robust
lysozyme
coating
layer
self-organized
on
Fe3O4@SiO2
nanoparticles
readily
mediated
heterogeneous
nucleation
growth
MOF
nanocrystals
to
form
magnetic
hierarchically
UIO-66(Ce/Ti)
core-shell
nanocomposites
(MHMUIO-66(Ce/Ti)
CSNCs).
The
MHMUIO-66(Ce/Ti)
CSNCs
exhibited
high
BET
surface
area
471.6
m2·g−
1
large
volume
0.39
cm3·g−
1,
central
13.7
nm
7.8
24.5
nm,
good
chemical
stability,
superparamagnetic
properties.
Consequently,
provided
accessibility
bimetallic
Ce/Ti
inside
binding
sites
thus
enhanced
phosphopeptides
enrichment
performance
low
detection
limit
(0.5
fmol)
selectivity
(mass
ratio
β -casein/BSA,
1:1000).
successful
identification
24
enriched
from
an
actual
sample
skim
milk
suggested
that
this
method
could
be
candidate
for
identifying
phosphopeptide
biomarkers
in
more
complex
biological
samples.
Research Square (Research Square), Год журнала: 2025, Номер unknown
Опубликована: Март 24, 2025
Язык: Английский