The Journal of Physical Chemistry B, Год журнала: 2025, Номер unknown

Опубликована: Май 14, 2025

DNA self-assembly of single molecules (i.e., dyes) with deterministic orientations is a powerful approach for engineering molecular excitons. However, current determination methods the dye orientation relative to do not account plane, which missing degree freedom needed define absolute three-dimensional orientations. In this work, we combine fluorescence-detected linear dichroism, defocused dipole imaging, and point accumulation imaging in nanoscale topography (DNA-PAINT) super-resolution microscopy determine Cy5 dyes host duplexes, includes plane orientation. The data revealed that absorption emission dipoles are perpendicular duplex, mean parallel bases, supports notion intercalate between base pairs. presented methodology will inspire investigation controlling arrangement configurations beyond spontaneous π-stacking as well achieving novel dye-DNA arrangements.

Язык: Английский