Extracellular vesicle as a next-generation drug delivery platform for rheumatoid arthritis therapy
Journal of Controlled Release,
Год журнала:
2025,
Номер
381, С. 113610 - 113610
Опубликована: Март 7, 2025
Язык: Английский
A New Strategy to Functionalize Exosomes via Enzymatic Engineering of Surface Glycans and its Application to Profile Exosomal Glycans and Endocytosis
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 19, 2025
Abstract
Exosomes
are
membrane‐enclosed
nanoparticles
secreted
by
cells
to
mediate
intercellular
communication.
Hence,
functionalized
exosomes
powerful
tools
in
biology
and
medicine,
efficient
methods
functionalize
highly
desired.
In
this
work,
a
novel
approach
is
developed
modify
based
on
enzymatic
engineering
of
their
surface
glycans.
It
employs
sialyltransferase
an
azide‐modified
sialyl
donor
enzymatically
install
azido‐sialic
acids
onto
exosomal
The
azide
tags
serve
as
universal
molecular
handles
attach
various
probes,
e.g.,
biotin,
protein,
fluorophore,
etc.,
simple
biocompatible
click
chemistry.
This
easy
effective,
the
modified
readily
retrieved
from
plate,
enabling
production
functional
practical
scales
for
studies
applications.
obtained
employed
profile
glycans,
disclosing
diverse
glycosylation
patterns
different
origins.
They
also
facilitated
comprehensive
investigations
cellular
uptake
disclose
macropinocytosis
main
general
route,
while
other
endocytosis
pathways
partially
involved
specific
exosomes.
Additionally,
new
exosome
functionalization
has
been
demonstrated
be
widely
applicable
Язык: Английский
Exosome-based therapeutics: Advancing drug delivery for neurodegenerative diseases
Molecular and Cellular Neuroscience,
Год журнала:
2025,
Номер
unknown, С. 104004 - 104004
Опубликована: Март 1, 2025
Язык: Английский
Engineered exosomes in service of tumor immunotherapy: From optimizing tumor‐derived exosomes to delivering CRISPR/Cas9 system
International Journal of Cancer,
Год журнала:
2024,
Номер
156(5), С. 898 - 913
Опубликована: Окт. 30, 2024
Exosomes
can
be
modified
and
designed
for
various
therapeutic
goals
because
of
their
unique
physical
chemical
characteristics.
Researchers
have
identified
tumor-derived
exosomes
(TEXs)
as
significant
players
in
cancer
by
influencing
tumor
growth,
immune
response
evasion,
angiogeneis,
drug
resistance.
TEXs
promote
the
production
specific
proteins
important
progression.
Due
to
easy
accessibility,
are
being
through
genetic,
delivery,
membrane,
system,
alterations
repurposed
vehicles
delivering
drugs
improve
treatment
outcomes.
In
complex
vivo
environment,
clustered
regularly
interspaced
short
palindromic
repeats
CRISPR-associated
protein
9
(CRISPR/Cas9)
system
encounters
challenges
from
degradation,
neutralization,
responses,
emphasizing
need
strategic
distribution
strategies
effective
genome
editing.
Engineered
present
a
promising
avenue
CRISPR/Cas9
vivo.
this
review,
we
will
explore
different
techniques
enhancing
using
engineering
strategies.
Additionally,
discuss
how
these
incorporated
into
advanced
genetic
systems
like
possible
uses.
Язык: Английский
Exosomes as novel nanocarriers for cancer therapy
Journal of Drug Delivery Science and Technology,
Год журнала:
2024,
Номер
101, С. 106262 - 106262
Опубликована: Окт. 9, 2024
Язык: Английский
Prolonged Immunomodulator Delivery Boosts Monocyte Exosome Secretion and Elevates Cathelicidin/LL-37 Content
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 799 - 810
Опубликована: Дек. 19, 2024
Human
cathelicidin
LL-37
offers
significant
benefits
to
the
immune
system
and
in
treating
various
diseases,
but
its
therapeutic
potential
is
hindered
by
low
activity
instability
physiological
environments.
Here,
we
introduce
a
strategy
boost
levels
exosomes
derived
from
THP-1
monocytes
incubating
cells
with
electrospun
nanofibers
containing
immunomodulators
(e.g.,
1α,
25-dihydroxyvitamin
D3
VID400).
Notably,
incubation
immunomodulator-loaded
not
only
increased
content
also
significantly
enhanced
production
of
engineered
exosomes.
Moreover,
these
demonstrated
multiple
biological
activities,
including
promoting
skin
cell
proliferation
migration,
enhancing
endothelial
tube
formation,
exhibiting
antibacterial
properties.
Collectively,
this
study
presents
an
approach
increasing
both
yield
their
content,
potentially
offering
promising
option
for
wound
healing,
tissue
regeneration,
infectious
disease
treatment.
Язык: Английский