Identification and Characterization of a Novel Multifunctional Terpene Synthase from the Pathogenic Fungus Cochliobolus heterostrophus DOI
Haichun Zeng, Bo Meng, Jiatong Zeng

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 22, 2025

Sesquiterpenes, a structurally diverse class of natural products, play pivotal roles in the pharmaceutical, fragrance, and flavor industries. In this study, we first identified functionally characterized multifunctional sesquiterpene synthase, ChTPS6, from plant-pathogenic fungus Cochliobolus heterostrophus. When incubated with FPP, ChTPS6 primarily catalyzed formation cubebol as main product, which is widely used sustained cooling refreshing agent food industry. Remarkably, exhibited substrate promiscuity, efficiently utilizing GPP GGPP alternative substrates. Moreover, through protein modeling site-directed mutagenesis, pinpointed key amino acid residues essential for catalytic activity successfully re-engineered enzyme to alter its product specificity. These findings provide novel insights into mechanism functional versatility while broadening potential applications biosynthesis valuable sesquiterpenes.

Язык: Английский

New insights into exploring new functional enzymes through the enzyme promiscuity DOI
Wenlong Liu,

Zhensong Wen,

Qing-yun Li

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 304, С. 140576 - 140576

Опубликована: Фев. 2, 2025

Язык: Английский

Процитировано

2
Mechanism of influence of nattokinase terminal sequence on catalytic performance and molecular modification DOI
Yuan Li, Hong Wang,

Kongfang Yu

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141872 - 141872

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1
Unleashing potential of microbial biosynthesis of monoterpenes via enzyme and metabolic engineering DOI

Dianqi Yang,

Hong Liang, Xuxu Li

и другие.

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108525 - 108525

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Beyond CEN.PK - parallel engineering of selected S. cerevisiae strains reveals that superior chassis strains require different engineering approaches for limonene production DOI Creative Commons

Yanmei Zhu,

Sasha Yogiswara,

Anke Willekens

и другие.

Metabolic Engineering, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Identification and Characterization of a Novel Multifunctional Terpene Synthase from the Pathogenic Fungus Cochliobolus heterostrophus DOI
Haichun Zeng, Bo Meng, Jiatong Zeng

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 22, 2025

Sesquiterpenes, a structurally diverse class of natural products, play pivotal roles in the pharmaceutical, fragrance, and flavor industries. In this study, we first identified functionally characterized multifunctional sesquiterpene synthase, ChTPS6, from plant-pathogenic fungus Cochliobolus heterostrophus. When incubated with FPP, ChTPS6 primarily catalyzed formation cubebol as main product, which is widely used sustained cooling refreshing agent food industry. Remarkably, exhibited substrate promiscuity, efficiently utilizing GPP GGPP alternative substrates. Moreover, through protein modeling site-directed mutagenesis, pinpointed key amino acid residues essential for catalytic activity successfully re-engineered enzyme to alter its product specificity. These findings provide novel insights into mechanism functional versatility while broadening potential applications biosynthesis valuable sesquiterpenes.

Язык: Английский

Процитировано

0