Biotechnology Advances,
Год журнала:
2022,
Номер
60, С. 107991 - 107991
Опубликована: Май 31, 2022
The
global
production
of
plastics
has
continuously
been
soaring
over
the
last
decades
due
to
their
extensive
use
in
our
daily
life
and
industries.
Although
synthetic
offer
great
advantages
from
packaging
construction
electronics,
low
biodegradability
induce
serious
plastic
pollution
that
damage
environment,
human
health
make
irreversible
changes
ecological
cycle.
In
particular,
containing
only
carbon-carbon
(C-C)
backbone
are
less
susceptible
degradation
lack
hydrolysable
groups.
representative
polyethylene
(PE)
polystyrene
(PS)
account
for
about
40%
total
production.
Various
chemical
biological
processes
with
potential
have
developed
recycle
reuse,
but
biodegradation
seems
be
most
attractive
eco-friendly
method
combat
this
growing
environmental
problem.
review,
we
first
summarize
current
advances
PE
PS
biodegradation,
including
isolation
microbes
degrading
enzymes
different
sources.
Next,
state-of-the-art
techniques
used
evaluating
monitoring
degradation,
scientific
toolboxes
enzyme
discovery
as
well
challenges
strategies
intensively
discussed.
return,
it
inspires
a
further
technological
exploration
expanding
diversity
species
enzymes,
disclosing
essential
pathways
developing
new
approaches
utilize
waste
feedstock
recycling
upcycling.
Polymer Engineering and Science,
Год журнала:
2020,
Номер
60(9), С. 2061 - 2075
Опубликована: Сен. 1, 2020
Abstract
Recently,
thoughtful
disagreements
between
scientists
concerning
environmental
issues
including
the
use
of
renewable
materials
have
enhanced
universal
awareness
biodegradable
materials.
Polylactic
acid
(PLA)
is
one
most
promising
for
commercially
replacing
nondegradable
such
as
polyethylene
terephthalate
and
polystyrene.
The
main
advantages
PLA
production
over
conventional
plastic
can
be
produced
from
resources
corn
or
other
carbohydrate
sources.
Besides,
provides
adequate
energy
saving
by
consuming
CO
2
during
production.
Thus,
we
aim
to
highlight
recent
research
involving
investigation
properties
PLA,
its
applications
four
types
potential
degradation
mechanisms.
In
first
part
article,
a
brief
discussion
problems
surrounding
provided
examples
polymers
currently
used
are
provided.
Next,
(Poly[L‐lactide]),
(Poly[D‐lactide])
(PDLA)
(Poly[DL‐lactide])
application
in
various
industries
packaging,
transportation,
agriculture
biomedical,
textile
electronic
industry
described.
Behaviors
subjected
hydrolytic,
photodegradative,
microbial
enzymatic
mechanisms
discussed
detail
latter
portion
article.
Frontiers in Microbiology,
Год журнала:
2020,
Номер
11
Опубликована: Апрель 21, 2020
Growing
accumulation
of
plastic
wastes
has
become
a
severe
environmental
and
social
issue.
It
is
urgent
to
develop
innovative
approaches
for
disposal
wastes.
Recently,
reports
on
biodegradation
synthetic
plastics
by
microorganisms
or
enzymes
spring
up,
which
arouses
our
great
enthusiasms
the
biological
treatment
technology
In
this
review,
we
comprehensively
summarize
that
have
been
reported
be
able
degrade
variety
generally
used
such
as
polyethylene
(PE),
polystyrene
(PS),
polypropylene
(PP),
polyvinyl
chloride
(PVC),
polyurethane
(PUR)
terephthalate
(PET).
addition,
highlight
microbial
metabolic
pathways
oligomers
monomers
styrene,
terephthalic
acid
(TPA)
ethylene
glycol
(EG),
current
attempts
towards
utilization
feedstocks
production
chemicals
with
high
value.
Taken
together,
these
findings
will
contribute
build
conception
bio-upcycling
connecting
biosynthesis
valuable
in
microorganisms.
Last
but
not
least,
discuss
challenges
toward
degradation
valorization
ACS Catalysis,
Год журнала:
2021,
Номер
11(3), С. 1340 - 1350
Опубликована: Янв. 13, 2021
Nature
has
provided
a
fantastic
array
of
enzymes
that
are
responsible
for
essential
biochemical
functions
but
not
usually
suitable
technological
applications.
Not
content
with
the
natural
repertoire,
protein
engineering
holds
promise
to
extend
applications
improved
tailored
properties.
However,
robust
proteins
remains
difficult
task
since
positive
mutation
library
may
cooperate
reach
target
function
in
most
cases
owing
ubiquity
epistatic
effects.
The
main
demand
lies
identifying
an
efficient
path
accumulated
mutations.
Herein,
we
devised
computational
strategy
(greedy
engineering,
GRAPE)
improve
robustness
PETase
from
Ideonella
sakaiensis.
A
systematic
clustering
analysis
combined
greedy
accumulation
beneficial
mutations
computationally
derived
enabled
redesign
variant,
DuraPETase,
which
exhibits
apparent
melting
temperature
is
drastically
elevated
by
31
°C
and
strikingly
enhanced
degradation
toward
semicrystalline
poly(ethylene
terephthalate)
(PET)
films
(30%)
at
mild
temperatures
(over
300-fold).
Complete
biodegradation
2
g/L
microplastics
water-soluble
products
under
conditions
also
achieved,
opening
up
opportunities
steer
biological
uncollectable
PET
waste
further
conversion
resulting
monomers
high-value
molecules.
crystal
structure
revealed
individual
match
design
model.
Concurrently,
synergistic
effects
captured,
while
interactions
alleviated
during
process.
We
anticipate
our
will
provide
broadly
applicable
global
optimization
enzyme
performance.
Proceedings of the National Academy of Sciences,
Год журнала:
2020,
Номер
117(41), С. 25476 - 25485
Опубликована: Сен. 28, 2020
Significance
Deconstruction
of
recalcitrant
polymers,
such
as
cellulose
or
chitin,
is
accomplished
in
nature
by
synergistic
enzyme
cocktails
that
evolved
over
millions
years.
In
these
systems,
soluble
dimeric
oligomeric
intermediates
are
typically
released
via
interfacial
biocatalysis,
and
additional
enzymes
often
process
the
into
monomers
for
microbial
uptake.
The
recent
discovery
a
two-enzyme
system
polyethylene
terephthalate
(PET)
deconstruction,
which
employs
one
to
convert
polymer
another
produce
constituent
PET
(MHETase),
suggests
may
be
evolving
similar
deconstruction
strategies
synthetic
plastics.
This
study
on
characterization
MHETase
synergy
depolymerization
inform
cocktail-based
plastics
upcycling.
Environmental Science and Ecotechnology,
Год журнала:
2020,
Номер
4, С. 100065 - 100065
Опубликована: Окт. 1, 2020
The
increased
global
demand
for
plastic
materials
has
led
to
severe
waste
pollution,
particularly
the
marine
environment.
This
critical
issue
affects
both
sea
life
and
human
beings
since
microplastics
can
enter
food
chain
cause
several
health
impacts.
Plastic
recycling,
chemical
treatments,
incineration
landfill
are
apparently
not
optimum
solutions
reducing
pollution.
Hence,
this
review
presents
two
newly
identified
environmentally
friendly
approaches,
biodegradation
bioplastic
production
using
algae,
solve
waste.
Algae,
microalgae,
degrade
through
toxins
systems
or
enzymes
synthesized
by
microalgae
itself
while
polymers
as
carbon
sources.
Utilizing
algae
been
critically
reviewed
in
paper
demonstrate
mechanism
how
affect
algae.
On
other
hand,
algae-derived
bioplastics
have
identical
properties
characteristics
petroleum-based
plastics,
remarkably
being
biodegradable
nature.
provides
new
insights
into
different
methods
of
producing
algae-based
(e.g.,
blending
with
genetic
engineering),
followed
discussion
on
challenges
further
research
direction
increase
their
commercial
feasibility.
