Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives DOI Creative Commons
Clodagh M. Carr, David J. Clarke, Alan D. W. Dobson

и другие.

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Ноя. 11, 2020

Since gaining popularity over 50 years ago, plastic has transformed our world, with many aspects of modern life relying on materials. However, the qualities which have made an attractive resource, such as ease mass production and advantageous strength-to-weight ratio, are equally responsible for damage that is typically caused once it becomes waste. In recent years, biological degradation emerged one way to address these unforeseen consequences. This strategy involves using microorganisms, primarily bacteria fungi, enzymes capable catalyzing degradative reactions, break apart into its original components. The focus this review will be microbial hydrolase found act polyethylene terephthalate or PET plastic, widely packaging synthetic fibers among other forms. best characterized examples discussed along use metagenomic protein engineering technologies in obtaining application. addition, obstacles currently limiting development efficient bioprocesses presented. By continuing study possible mechanisms key enzyme structural elements behind hydrolysis assessing ability under practical conditions, research can progress towards large-scale waste management operations. Finally, contribution hydrolases creating a circular economy explored

Язык: Английский

Machine learning-aided engineering of hydrolases for PET depolymerization DOI
Hongyuan Lu, Daniel J. Diaz, Natalie J. Czarnecki

и другие.

Nature, Год журнала: 2022, Номер 604(7907), С. 662 - 667

Опубликована: Апрель 27, 2022

Язык: Английский

Процитировано

765

Plastic biodegradation: Frontline microbes and their enzymes DOI
Ayodeji Amobonye, Prashant Bhagwat, Suren Singh

и другие.

The Science of The Total Environment, Год журнала: 2020, Номер 759, С. 143536 - 143536

Опубликована: Ноя. 6, 2020

Язык: Английский

Процитировано

530

Microbial Degradation and Valorization of Plastic Wastes DOI Creative Commons

Jiakang Ru,

Yi‐Xin Huo, Yu Yang

и другие.

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Апрель 21, 2020

Growing accumulation of plastic wastes has become a severe environmental and social issue. It is urgent to develop innovative approaches for disposal wastes. Recently, reports on biodegradation synthetic plastics by microorganisms or enzymes spring up, which arouses our great enthusiasms the biological treatment technology In this review, we comprehensively summarize that have been reported be able degrade variety generally used such as polyethylene (PE), polystyrene (PS), polypropylene (PP), polyvinyl chloride (PVC), polyurethane (PUR) terephthalate (PET). addition, highlight microbial metabolic pathways oligomers monomers styrene, terephthalic acid (TPA) ethylene glycol (EG), current attempts towards utilization feedstocks production chemicals with high value. Taken together, these findings will contribute build conception bio-upcycling connecting biosynthesis valuable in microorganisms. Last but not least, discuss challenges toward degradation valorization

Язык: Английский

Процитировано

459

Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy DOI
Yinglu Cui, Yanchun Chen,

Xinyue Liu

и другие.

ACS Catalysis, Год журнала: 2021, Номер 11(3), С. 1340 - 1350

Опубликована: Янв. 13, 2021

Nature has provided a fantastic array of enzymes that are responsible for essential biochemical functions but not usually suitable technological applications. Not content with the natural repertoire, protein engineering holds promise to extend applications improved tailored properties. However, robust proteins remains difficult task since positive mutation library may cooperate reach target function in most cases owing ubiquity epistatic effects. The main demand lies identifying an efficient path accumulated mutations. Herein, we devised computational strategy (greedy engineering, GRAPE) improve robustness PETase from Ideonella sakaiensis. A systematic clustering analysis combined greedy accumulation beneficial mutations computationally derived enabled redesign variant, DuraPETase, which exhibits apparent melting temperature is drastically elevated by 31 °C and strikingly enhanced degradation toward semicrystalline poly(ethylene terephthalate) (PET) films (30%) at mild temperatures (over 300-fold). Complete biodegradation 2 g/L microplastics water-soluble products under conditions also achieved, opening up opportunities steer biological uncollectable PET waste further conversion resulting monomers high-value molecules. crystal structure revealed individual match design model. Concurrently, synergistic effects captured, while interactions alleviated during process. We anticipate our will provide broadly applicable global optimization enzyme performance.

Язык: Английский

Процитировано

442

Characterization and engineering of a two-enzyme system for plastics depolymerization DOI Creative Commons
Brandon C. Knott, Erika Erickson, Mark D. Allen

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2020, Номер 117(41), С. 25476 - 25485

Опубликована: Сен. 28, 2020

Significance Deconstruction of recalcitrant polymers, such as cellulose or chitin, is accomplished in nature by synergistic enzyme cocktails that evolved over millions years. In these systems, soluble dimeric oligomeric intermediates are typically released via interfacial biocatalysis, and additional enzymes often process the into monomers for microbial uptake. The recent discovery a two-enzyme system polyethylene terephthalate (PET) deconstruction, which employs one to convert polymer another produce constituent PET (MHETase), suggests may be evolving similar deconstruction strategies synthetic plastics. This study on characterization MHETase synergy depolymerization inform cocktail-based plastics upcycling.

Язык: Английский

Процитировано

410

Expanding plastics recycling technologies: chemical aspects, technology status and challenges DOI Creative Commons
Houqian Li, Horacio A. Aguirre‐Villegas, Robert D. Allen

и другие.

Green Chemistry, Год журнала: 2022, Номер 24(23), С. 8899 - 9002

Опубликована: Янв. 1, 2022

This paper reviewed the entire life cycle of plastics and options for management plastic waste to address barriers industrial chemical recycling further provide perceptions on possible opportunities with such materials.

Язык: Английский

Процитировано

351

Enzymatic degradation of plant biomass and synthetic polymers DOI
Chun‐Chi Chen, Longhai Dai, Lixin Ma

и другие.

Nature Reviews Chemistry, Год журнала: 2020, Номер 4(3), С. 114 - 126

Опубликована: Фев. 21, 2020

Язык: Английский

Процитировано

324

Enzymes’ Power for Plastics Degradation DOI
Vincent Tournier, Sophie Duquesne,

Frédérique Guillamot

и другие.

Chemical Reviews, Год журнала: 2023, Номер 123(9), С. 5612 - 5701

Опубликована: Март 14, 2023

Plastics are everywhere in our modern way of living, and their production keeps increasing every year, causing major environmental concerns. Nowadays, the end-of-life management involves accumulation landfills, incineration, recycling to a lower extent. This ecological threat environment is inspiring alternative bio-based solutions for plastic waste treatment toward circular economy. Over past decade, considerable efforts have been made degrade commodity plastics using biocatalytic approaches. Here, we provide comprehensive review on recent advances enzyme-based biocatalysis design related processes recycle or upcycle plastics, including polyesters, polyamides, polyurethanes, polyolefins. We also discuss scope limitations, challenges, opportunities this field research. An important message from that polymer-assimilating enzymes very likely part solution reaching

Язык: Английский

Процитировано

285

Techno-economic, life-cycle, and socioeconomic impact analysis of enzymatic recycling of poly(ethylene terephthalate) DOI Creative Commons
Avantika Singh, Nicholas A. Rorrer, Scott Nicholson

и другие.

Joule, Год журнала: 2021, Номер 5(9), С. 2479 - 2503

Опубликована: Июль 15, 2021

Esterases have emerged as important biocatalysts for enzyme-based polyester recycling of poly(ethylene terephthalate) (PET) to terephthalic acid (TPA) and ethylene glycol (EG). Here, we present process modeling, techno-economic, life-cycle, socioeconomic impact analyses an enzymatic PET depolymerization-based process, which compare with virgin TPA manufacturing. We predict that enzymatically recycled (rTPA) can be cost-competitive highlight key areas achieve this. In addition favorable long-term benefits, rTPA reduce total supply chain energy use by 69%–83% greenhouse gas emissions 17%–43% per kg TPA. An economy-wide assessment the US estimates environmental impacts up 95% while generating 45% more also relative production. Sensitivity impactful research opportunities pursue toward realizing biological upcycling.

Язык: Английский

Процитировано

275

Directed evolution of an efficient and thermostable PET depolymerase DOI
Elizabeth L. Bell, Ross Smithson,

Siobhan Kilbride

и другие.

Nature Catalysis, Год журнала: 2022, Номер 5(8), С. 673 - 681

Опубликована: Авг. 11, 2022

Язык: Английский

Процитировано

268