1,4,5-Trisubstituted-1,2,3-Triazoles: An Unconventional Class of Ribonuclease A Inhibitors DOI

Rashmita Pan,

Swagata Dasgupta, Tanmaya Pathak

и другие.

Organic & Biomolecular Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

The "catalytic triad" present at the active site of ribonuclease A (RNase A) is responsible for cleavage 5'-phosphodiester bond; amino acid residues His12, Lys41 and His119 constituting this triad provide a positively charged environment physiological pH. Based on docking studies, 1,4,5-trisubstituted-carboxylated 1,2,3-triazoles (1,4,5-TTs) were identified as new class RNase inhibitors. Therefore, two different groups 1,4,5-TTs, functionalized with carboxylic groups, synthesized by reacting pre butyne-1,4-diol derivatives several aryl/alkyl azides under solvent catalyst free conditions. Inhibitory properties molecules heteroatom linked "CH

Язык: Английский

Current trends and future potential in the detection of avian coronaviruses: An emphasis on sensors-based technologies. DOI Creative Commons
Md. Safiul Alam Bhuiyan, Suman Das Gupta,

Juplikely James Silip

и другие.

Virology, Год журнала: 2025, Номер 604, С. 110399 - 110399

Опубликована: Янв. 18, 2025

Infectious bronchitis virus (IBV), an avian coronavirus, member of the genus Gammacoronavirus, poses significant threats to poultry health, causing severe respiratory, reproductive, and renal infections. The genetic diversity IBV, driven by mutations, recombination deletions, has led emergence numerous serotypes genotypes, complicating both diagnosis control measures. Rapid accurate diagnostic tools are essential for effective disease management minimizing economic losses. Conventional methods, such as PCR, isolation, serological assays, hindered limitations in sensitivity, specificity, turnaround time. In contrast, innovative biosensor platforms employing advanced detection mechanisms-including electrochemical, optical, piezoelectric sensors-offer a transformative solution. These technologies provide portable, highly sensitive, rapid IBV detection. Beyond addressing challenges conventional these biosensor-based approaches facilitate real-time monitoring enhance surveillance. This review highlights potential biosensors their integration into strategies coronavirus infections, presenting them promising alternative precise efficient

Язык: Английский

Процитировано

1

A Comprehensive Review on Circulating cfRNA in Plasma: Implications for Disease Diagnosis and Beyond DOI Creative Commons

Pengqiang Zhong,

Lu Bai,

Mengzhi Hong

и другие.

Diagnostics, Год журнала: 2024, Номер 14(10), С. 1045 - 1045

Опубликована: Май 17, 2024

Circulating cfRNA in plasma has emerged as a fascinating area of research with potential applications disease diagnosis, monitoring, and personalized medicine. RNA sequencing technology allows for the non-invasive collection important information about expression target genes, eliminating need biopsies. This comprehensive review aims to provide detailed overview current knowledge advancements study cfRNA, focusing on its diverse landscape biological functions, detection methods, diagnostic prognostic various diseases, challenges, future perspectives.

Язык: Английский

Процитировано

6

Novel mechanistic insights – A brand new Era for anti-HBV drugs DOI

Weiping Lyu,

Haoming Qin,

Qi Li

и другие.

European Journal of Medicinal Chemistry, Год журнала: 2024, Номер 279, С. 116854 - 116854

Опубликована: Сен. 13, 2024

Язык: Английский

Процитировано

4

19F-NMR in RNA structural biology: exploring structures, dynamics, and small molecule interactions DOI
CongBao Kang

European Journal of Medicinal Chemistry, Год журнала: 2025, Номер 292, С. 117682 - 117682

Опубликована: Апрель 23, 2025

Язык: Английский

Процитировано

0

Non-oncogene dependencies: Novel opportunities for cancer therapy DOI
Tiziana Di Marco, Mara Mazzoni, Angela Greco

и другие.

Biochemical Pharmacology, Год журнала: 2024, Номер 228, С. 116254 - 116254

Опубликована: Май 3, 2024

Язык: Английский

Процитировано

3

Apollon: a deoxyribozyme that generates a yellow product DOI Creative Commons
Martin Volek,

Jaroslav Kurfürst,

Milan Kožíšek

и другие.

Nucleic Acids Research, Год журнала: 2024, Номер 52(15), С. 9062 - 9075

Опубликована: Июнь 13, 2024

Abstract Colorimetric assays in which the color of a solution changes presence an input provide simple and inexpensive way to monitor experimental readouts. In this study we used vitro selection identify self-phosphorylating kinase deoxyribozyme that produces colorimetric signal by converting colorless substrate pNPP into yellow product pNP. The minimized catalytic core, sequence requirements, secondary structure, buffer requirements deoxyribozyme, named Apollon, were characterized using variety techniques including reselection experiments, high-throughput sequencing, comparative analysis, biochemical activity assays, NMR. A bimolecular version Apollon catalyzed multiple turnover phosphorylation amplified signal. Engineered versions could detect oligonucleotides with specific sequences as well several different types nucleases homogenous can be performed single tube without need for washes or purifications. We anticipate will particularly useful reduce costs screens applications specialized equipment is not available.

Язык: Английский

Процитировано

2

1,4,5-Trisubstituted-1,2,3-Triazoles: An Unconventional Class of Ribonuclease A Inhibitors DOI

Rashmita Pan,

Swagata Dasgupta, Tanmaya Pathak

и другие.

Organic & Biomolecular Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

The "catalytic triad" present at the active site of ribonuclease A (RNase A) is responsible for cleavage 5'-phosphodiester bond; amino acid residues His12, Lys41 and His119 constituting this triad provide a positively charged environment physiological pH. Based on docking studies, 1,4,5-trisubstituted-carboxylated 1,2,3-triazoles (1,4,5-TTs) were identified as new class RNase inhibitors. Therefore, two different groups 1,4,5-TTs, functionalized with carboxylic groups, synthesized by reacting pre butyne-1,4-diol derivatives several aryl/alkyl azides under solvent catalyst free conditions. Inhibitory properties molecules heteroatom linked "CH

Язык: Английский

Процитировано

0