Current trends and future potential in the detection of avian coronaviruses: An emphasis on sensors-based technologies.
Virology,
Год журнала:
2025,
Номер
604, С. 110399 - 110399
Опубликована: Янв. 18, 2025
Infectious
bronchitis
virus
(IBV),
an
avian
coronavirus,
member
of
the
genus
Gammacoronavirus,
poses
significant
threats
to
poultry
health,
causing
severe
respiratory,
reproductive,
and
renal
infections.
The
genetic
diversity
IBV,
driven
by
mutations,
recombination
deletions,
has
led
emergence
numerous
serotypes
genotypes,
complicating
both
diagnosis
control
measures.
Rapid
accurate
diagnostic
tools
are
essential
for
effective
disease
management
minimizing
economic
losses.
Conventional
methods,
such
as
PCR,
isolation,
serological
assays,
hindered
limitations
in
sensitivity,
specificity,
turnaround
time.
In
contrast,
innovative
biosensor
platforms
employing
advanced
detection
mechanisms-including
electrochemical,
optical,
piezoelectric
sensors-offer
a
transformative
solution.
These
technologies
provide
portable,
highly
sensitive,
rapid
IBV
detection.
Beyond
addressing
challenges
conventional
these
biosensor-based
approaches
facilitate
real-time
monitoring
enhance
surveillance.
This
review
highlights
potential
biosensors
their
integration
into
strategies
coronavirus
infections,
presenting
them
promising
alternative
precise
efficient
Язык: Английский
A Comprehensive Review on Circulating cfRNA in Plasma: Implications for Disease Diagnosis and Beyond
Pengqiang Zhong,
Lu Bai,
Mengzhi Hong
и другие.
Diagnostics,
Год журнала:
2024,
Номер
14(10), С. 1045 - 1045
Опубликована: Май 17, 2024
Circulating
cfRNA
in
plasma
has
emerged
as
a
fascinating
area
of
research
with
potential
applications
disease
diagnosis,
monitoring,
and
personalized
medicine.
RNA
sequencing
technology
allows
for
the
non-invasive
collection
important
information
about
expression
target
genes,
eliminating
need
biopsies.
This
comprehensive
review
aims
to
provide
detailed
overview
current
knowledge
advancements
study
cfRNA,
focusing
on
its
diverse
landscape
biological
functions,
detection
methods,
diagnostic
prognostic
various
diseases,
challenges,
future
perspectives.
Язык: Английский
Novel mechanistic insights – A brand new Era for anti-HBV drugs
Weiping Lyu,
Haoming Qin,
Qi Li
и другие.
European Journal of Medicinal Chemistry,
Год журнала:
2024,
Номер
279, С. 116854 - 116854
Опубликована: Сен. 13, 2024
Язык: Английский
19F-NMR in RNA structural biology: exploring structures, dynamics, and small molecule interactions
European Journal of Medicinal Chemistry,
Год журнала:
2025,
Номер
292, С. 117682 - 117682
Опубликована: Апрель 23, 2025
Язык: Английский
Non-oncogene dependencies: Novel opportunities for cancer therapy
Biochemical Pharmacology,
Год журнала:
2024,
Номер
228, С. 116254 - 116254
Опубликована: Май 3, 2024
Язык: Английский
Apollon: a deoxyribozyme that generates a yellow product
Nucleic Acids Research,
Год журнала:
2024,
Номер
52(15), С. 9062 - 9075
Опубликована: Июнь 13, 2024
Abstract
Colorimetric
assays
in
which
the
color
of
a
solution
changes
presence
an
input
provide
simple
and
inexpensive
way
to
monitor
experimental
readouts.
In
this
study
we
used
vitro
selection
identify
self-phosphorylating
kinase
deoxyribozyme
that
produces
colorimetric
signal
by
converting
colorless
substrate
pNPP
into
yellow
product
pNP.
The
minimized
catalytic
core,
sequence
requirements,
secondary
structure,
buffer
requirements
deoxyribozyme,
named
Apollon,
were
characterized
using
variety
techniques
including
reselection
experiments,
high-throughput
sequencing,
comparative
analysis,
biochemical
activity
assays,
NMR.
A
bimolecular
version
Apollon
catalyzed
multiple
turnover
phosphorylation
amplified
signal.
Engineered
versions
could
detect
oligonucleotides
with
specific
sequences
as
well
several
different
types
nucleases
homogenous
can
be
performed
single
tube
without
need
for
washes
or
purifications.
We
anticipate
will
particularly
useful
reduce
costs
screens
applications
specialized
equipment
is
not
available.
Язык: Английский
1,4,5-Trisubstituted-1,2,3-Triazoles: An Unconventional Class of Ribonuclease A Inhibitors
Organic & Biomolecular Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 18, 2024
The
"catalytic
triad"
present
at
the
active
site
of
ribonuclease
A
(RNase
A)
is
responsible
for
cleavage
5'-phosphodiester
bond;
amino
acid
residues
His12,
Lys41
and
His119
constituting
this
triad
provide
a
positively
charged
environment
physiological
pH.
Based
on
docking
studies,
1,4,5-trisubstituted-carboxylated
1,2,3-triazoles
(1,4,5-TTs)
were
identified
as
new
class
RNase
inhibitors.
Therefore,
two
different
groups
1,4,5-TTs,
functionalized
with
carboxylic
groups,
synthesized
by
reacting
pre
butyne-1,4-diol
derivatives
several
aryl/alkyl
azides
under
solvent
catalyst
free
conditions.
Inhibitory
properties
molecules
heteroatom
linked
"CH
Язык: Английский