Naturally
occurring
fluorescent
proteins
(FPs)
are
the
most
widely
used
tools
for
tracking
cellular
and
sensing
events.
Here,
we
chemically
evolved
self-labeling
SNAP-tag
into
a
palette
of
mimics
(SmFPs)
that
possess
bright,
rapidly
inducible
fluorescence
ranging
from
cyan
to
infrared.
SmFPs
integral
chemical-genetic
entities
based
on
same
fluorogenic
principle
as
FPs,
i.e.,
induction
non-emitting
molecular
rotors
by
conformational
locking.
We
demonstrate
usefulness
these
in
real-time
protein
expression,
degradation,
binding
interactions,
trafficking,
assembly,
show
optimally
designed
outperform
FPs
like
GFP
many
important
ways.
further
circularly
permuted
is
sensitive
changes
their
fusion
partners,
partners
can
be
development
single
SmFP-based
genetically
encoded
calcium
sensors
live
cell
imaging.
Journal of the American Chemical Society,
Год журнала:
2022,
Номер
144(25), С. 11075 - 11080
Опубликована: Июнь 13, 2022
Photoconvertible
tracking
strategies
assess
the
dynamic
migration
of
cell
populations.
Here
we
develop
phototruncation-assisted
(PACT)
and
apply
it
to
evaluate
immune
cells
into
tumor-draining
lymphatics.
This
method
is
enabled
by
a
recently
discovered
cyanine
photoconversion
reaction
that
leads
two-carbon
truncation
consequent
blue-shift
these
commonly
used
probes.
By
examining
substituent
effects
on
heptamethine
chromophore,
find
introduction
single
methoxy
group
increases
yield
phototruncation
in
neutral
buffer
almost
8-fold.
When
converted
membrane-bound
cell-tracking
variant,
this
probe
can
be
applied
series
vitro
vivo
experiments.
These
include
quantitative,
time-dependent
measurements
from
tumors
lymph
nodes.
Unlike
previously
reported
cellular
approaches,
does
not
require
genetic
engineering
uses
near-infrared
(NIR)
wavelengths.
Overall,
PACT
provides
straightforward
approach
label
populations
with
spatiotemporal
control.
Chemical Science,
Год журнала:
2024,
Номер
15(11), С. 3800 - 3830
Опубликована: Янв. 1, 2024
Self-assembly
has
been
considered
as
a
strategy
to
construct
superstructures
with
specific
functions,
which
widely
used
in
many
different
fields,
such
bionics,
catalysis,
and
pharmacology.
A
detailed
in-depth
analysis
of
the
self-assembly
mechanism
is
beneficial
for
directionally
accurately
regulating
process
substances.
Fluorescent
probes
exhibit
unique
advantages
sensitivity,
non-destructiveness,
real-time
tracking,
compared
traditional
methods.
In
this
work,
design
principle
fluorescent
functions
their
applications
detection
thermodynamic
kinetic
parameters
during
were
systematically
reviewed.
Their
efficiency,
limitations
are
also
discussed.
Furthermore,
promising
perspectives
investigating
discussed
suggested.
ACS Nano,
Год журнала:
2021,
Номер
15(8), С. 12483 - 12496
Опубликована: Июль 26, 2021
The
rise
of
single-molecule
localization
microscopy
(SMLM)
and
related
super-resolution
methods
over
the
past
15
years
has
revolutionized
how
we
study
biological
materials
systems.
In
this
Perspective,
reflect
on
underlying
philosophy
diffraction-unlimited
pictures
containing
rich
spatial
functional
information
may
gradually
emerge
through
local
accumulation
measurements.
Starting
with
basic
concepts,
analyze
uniqueness
opportunities
in
building
up
final
picture
one
molecule
at
a
time.
After
brief
introductions
to
more
established
multicolor
three-dimensional
measurements,
highlight
emerging
efforts
extend
SMLM
new
dimensions
functionalities
as
fluorescence
polarization,
emission
spectra,
molecular
motions,
discuss
rising
future
directions.
With
single
molecules
our
quanta,
bottom-up
approach
provides
powerful
conduit
for
multidimensional
nanoscale.
Naturally
occurring
fluorescent
proteins
(FPs)
are
the
most
widely
used
tools
for
tracking
cellular
and
sensing
events.
Here,
we
chemically
evolved
self-labeling
SNAP-tag
into
a
palette
of
mimics
(SmFPs)
that
possess
bright,
rapidly
inducible
fluorescence
ranging
from
cyan
to
infrared.
SmFPs
integral
chemical-genetic
entities
based
on
same
fluorogenic
principle
as
FPs,
i.e.,
induction
non-emitting
molecular
rotors
by
conformational
locking.
We
demonstrate
usefulness
these
in
real-time
protein
expression,
degradation,
binding
interactions,
trafficking,
assembly,
show
optimally
designed
outperform
FPs
like
GFP
many
important
ways.
further
circularly
permuted
is
sensitive
changes
their
fusion
partners,
partners
can
be
development
single
SmFP-based
genetically
encoded
calcium
sensors
live
cell
imaging.
