Effects of arsenic exposure on blood trace element levels in rats and sex differences

BioMetals, Год журнала: 2024, Номер unknown

Опубликована: Апрель 3, 2024

Язык: Английский

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Proteomic analysis of the effects of Dictyophora polysaccharide on arsenic-induced hepatotoxicity in rats

Experimental and Molecular Pathology, Год журнала: 2024, Номер 138, С. 104910 - 104910

Опубликована: Июнь 13, 2024

Arsenic (As) is a highly toxic environmental toxicant and known human carcinogen. Long-term exposure to As can cause liver injury. Dictyophora polysaccharide (DIP) biologically active natural compound found in the with excellent antioxidation, anti-inflammation, immune protection properties. In this study, Sprague-Dawley (SD) rat model of toxicity was established using feeding method, followed by DIP treatment rats As-induced The molecular mechanisms protective effect were investigated proteomic studies. results showed that 172, 328 191 differentially expressed proteins (DEPs) identified between As-exposed versus control (As/Ctrl), treated (DIP+As/As), (DIP+As /Ctrl), respectively. Among them, expression 90 DEPs As/Ctrl groups reversed treatment. caused dysregulation metabolic pathways, mitochondria, oxidative stress, apoptosis-related liver. However, changed or restored levels these proteins, which attenuated damage livers exposure. provide new insights into injury induced poisoning.

Язык: Английский

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Integration of proteomics and metabolomics analysis investigate mechanism of As-induced immune injury in rat spleen

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 284, С. 116913 - 116913

Опубликована: Авг. 28, 2024

Arsenic (As) is a widespread metalloid and human carcinogen found in the natural environment, multiple toxic effects have been shown to be associated with As exposure. can accumulated spleen, largest peripheral lymphatic organ, long-term exposure lead splenic injury. In this study, Sprague-Dawley (SD) rat model of As-poisoned was established, aiming explore molecular mechanism As-induced immune injury through combined analysis proteomics metabolomics rats' spleen. After feeding rats diet (50 mg/kg) for 90 days, spleen tissue group damaged, level significantly higher than that control (P < 0.001), inflammatory cytokine interleukin-6 (IL-6) decreased 0.01). Proteomics results showed total 134 differentially expressed proteins (DEPs) 0.05 fold change > 1.2) 182 metabolites (DEMs) (VIP >1 P 0.05) were identified spleens poisoned compared (As/Ctrl). The proteomic highlight role hypoxia-inducible factors (HIF), killer cell mediated cytotoxicity, ribosomes. major pathways metabolic disruption included arachidonic acid (AA) metabolism, glycerophospholipid metabolism folate single-carbon pool. integrated these two omics suggested Hmox1, Stat3, acid, phosphatidylcholine leukotriene B4 may play key roles by indicate cause damage rats. Through metabolomic analysis, their mechanisms obtained, which provided basis further understanding caused

Язык: Английский

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Study on the Mechanism of Dictyophora duplicata Polysaccharide in Reducing Depression-like Behavior in Mice

Nutrients, Год журнала: 2024, Номер 16(21), С. 3785 - 3785

Опубликована: Ноя. 4, 2024

Background/Objectives: Depression is a prevalent worldwide mental health disorder that inflicts significant harm to individuals and society. Dictyophora duplicata an edible fungus contains variety of nutrients, including polysaccharides. This study aims investigate the monosaccharide composition molecular weight polysaccharide (DDP-B1), followed by exploration its antidepressant effects in chronic unpredictable mild stress (CUMS) mice. Methods: was purified using DEAE-52 column S-400 obtain DDP-B1. The DDP-B1 were investigated via high-performance gel permeation chromatograph. Six-week-old C57BL/6 male mice utilized for CUMS modeling evaluate efficacy Fluoxetine served as positive control group. depressive-like behaviors brain pathology evaluated. Immunofluorescence (IF) staining, metabolomics analysis, western blot employed further underlying mechanisms. Results: significantly alleviated depression-like behavior increased expression SYN PSD-95 mice’s brains, which validated blot. Metabolomics analysis indicated reduction serum glutamate following treatment. Moreover, treatment led increase levels GABAAR, BDNF, p-TrkB p-p70S6K. Conclusions: regulated abnormalities glutamatergic system, subsequently activated BDNF-TrkB-mTOR pathway mitigated pathological manifestations potential medication established theoretical foundation development fungi with similar properties.

Язык: Английский

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Molecular Disturbances and Thyroid Gland Dysfunction in Rats Chronically Exposed to a High Dose of NaAsO₂: Insights from Proteomic and Phosphoproteomic Analyses

Journal of Hazardous Materials, Год журнала: 2024, Номер 484, С. 136746 - 136746

Опубликована: Дек. 3, 2024

Язык: Английский

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