Capsid and genome damage are the leading inactivation mechanisms of aerosolized porcine respiratory coronavirus at different relative humidities DOI Creative Commons

Aijia Zhou,

P. S. Ganesh Subramanian,

Salma El-Naggar

и другие.

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

ABSTRACT Relative humidity (RH) varies widely in indoor environments based on temperature, outdoor humidity, heating systems, and other environmental conditions. This study explored how RH affects aerosolized porcine respiratory coronavirus (PRCV), a model for coronaviruses, over time range from 0 min to maximum of 1 h, the molecular mechanism behind viral infectivity reduction. These questions were answered by quantifying: (i) viral-host receptor interactions, (ii) capsid integrity, (iii) genome (iv) virus infectivity. We found did not alter PRCV-receptor interactions. RHs 45–55% 65–75% damaged genomes (2 log 10 reduction reduction, respectively, terms median sample value), whereas 55–65% decreased integrity reduction). No apparent virion damage was observed 75–85%. Two assays used quantify presence: qPCR detecting plaque-forming unit assay replication. Our results indicated that overestimated concentrations infectious viruses, RNase treatment with long-range RT-qPCR performed better than one-step RT-qPCR. propose understanding influence stability viruses provides critical information preventing transmission coronaviruses. IMPORTANCE Indoor can impact which then affect rates. The mechanisms relative still remain unclear. inactivates damaging its genome. finding highlights potential role controlling levels as strategy reduce risk transmission.

Язык: Английский

Capsid and genome damage are the leading inactivation mechanisms of aerosolized porcine respiratory coronavirus at different relative humidities DOI Creative Commons

Aijia Zhou,

P. S. Ganesh Subramanian,

Salma El-Naggar

и другие.

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

ABSTRACT Relative humidity (RH) varies widely in indoor environments based on temperature, outdoor humidity, heating systems, and other environmental conditions. This study explored how RH affects aerosolized porcine respiratory coronavirus (PRCV), a model for coronaviruses, over time range from 0 min to maximum of 1 h, the molecular mechanism behind viral infectivity reduction. These questions were answered by quantifying: (i) viral-host receptor interactions, (ii) capsid integrity, (iii) genome (iv) virus infectivity. We found did not alter PRCV-receptor interactions. RHs 45–55% 65–75% damaged genomes (2 log 10 reduction reduction, respectively, terms median sample value), whereas 55–65% decreased integrity reduction). No apparent virion damage was observed 75–85%. Two assays used quantify presence: qPCR detecting plaque-forming unit assay replication. Our results indicated that overestimated concentrations infectious viruses, RNase treatment with long-range RT-qPCR performed better than one-step RT-qPCR. propose understanding influence stability viruses provides critical information preventing transmission coronaviruses. IMPORTANCE Indoor can impact which then affect rates. The mechanisms relative still remain unclear. inactivates damaging its genome. finding highlights potential role controlling levels as strategy reduce risk transmission.

Язык: Английский

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