PHA Microplastic Aging Decreases N₂O Sink Capacity: Released γ-Butyrolactone Decouples Denitrifying Electron Transfer and Oxidative Phosphorylation

Environmental Science & Technology, Год журнала: 2025, Номер 59(2), С. 1298 - 1307

Опубликована: Янв. 9, 2025

Bacterial denitrification is a main pathway for soil N2O sinks, which crucial assessing and controlling emissions. Biobased polyhydroxyalkanoate (PHA) microplastic particles (MPs) degrade slowly in conventional environments, remaining inert extended periods. However, the impacts of PHA aging on bacterial sink capacity before degradation remain poorly understood. Here, model strain Paracoccus denitrificans was exposed to 0.05-0.5% (w/w) virgin aged MPs. Although no significant changes molecular weights were observed, MPs hindered cell growth reduction rates, leading surge 1H NMR spectroscopy UPLC-QTOF-MS analysis identified γ-butyrolactone as key component released from Metabolic verifications at cellular level confirmed its inhibition ATP synthesis. The that protonated hydrolyzed spontaneously periplasm would compete protons with ATPase destroy coupling between denitrifying electron transfer oxidative phosphorylation. Consequently, energy-deficient cells reduced supply reduction, did not contribute energy conservation. This work unveils novel mechanism by impairs highlights need consider environmental risks posed biobased aging.

Язык: Английский

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Biodegradable Microplastics Increase N₂O Emission from Denitrifying Sludge More Than Conventional Microplastics

Environmental Science & Technology Letters, Год журнала: 2024, Номер unknown

Опубликована: Июнь 10, 2024

Despite the increasing concern about impacts of microplastics on wastewater treatment, underlying mechanism by which affect nitrous oxide (N2O) accumulation during denitrification is still underexplored. In particular, effects biodegradable (BMPs) sewage sludge systems are largely overlooked. Previous studies often used one type polymer as model microplastics, far from a real-world scenario various occurring simultaneously. This work assesses toxic influences chronically adding four typical BMPs versus conventional nonbiodegradable (NBMPs) to denitrifying sludge. Our results showed that both and NBMPs suppressed performance, intensified electron competition, regulated distribution, consequently promoted N2O at chemical oxygen demand:nitrate ratio <4:1. Importantly, more severe were observed in reactor with BMPs. A subsequent mechanistic study revealed significantly decreased relative abundances denitrifiers key genes involved transport consumption system (ETCS), might be related varied extracellular polymeric substance components. contrast, damaged bacterial membranes directly caused dead cells overproducing reactive species, hence disrupting ETCS. Overall, this suggested wastewater, especially BMPs, could disrupt potentially increase greenhouse gas emission.

Язык: Английский

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Research advances of biodegradable microplastics in wastewater treatment plant: Current knowledge and future directions

The Science of The Total Environment, Год журнала: 2024, Номер 951, С. 175614 - 175614

Опубликована: Авг. 18, 2024

Язык: Английский

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Biodegradable microplastics aggravate greenhouse gas emissions from urban lake sediments more severely than conventional microplastics

Water Research, Год журнала: 2024, Номер 266, С. 122334 - 122334

Опубликована: Авг. 25, 2024

Язык: Английский

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Leaching behavior of microplastics during sludge mechanical dewatering and its effect on activated sludge

Water Research, Год журнала: 2024, Номер 266, С. 122395 - 122395

Опубликована: Сен. 3, 2024

Язык: Английский

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Responses of nitrogen removal, microbial community and antibiotic resistance genes to biodegradable microplastics during biological wastewater treatment

Biochemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 109732 - 109732

Опубликована: Март 1, 2025

Язык: Английский

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Insights into the photoaging behavior of biodegradable and nondegradable microplastics: spectroscopic and molecular characteristics of dissolved organic matter release

Journal of Hazardous Materials, Год журнала: 2024, Номер 483, С. 136651 - 136651

Опубликована: Ноя. 24, 2024

Язык: Английский

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Characteristics and nontargeted metabolomics analysis of anammox granular sludge under short-term exposure to polypropylene and polylactic acid microplastics

International Biodeterioration & Biodegradation, Год журнала: 2024, Номер 195, С. 105891 - 105891

Опубликована: Авг. 27, 2024

Язык: Английский

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