In Vitro Antileishmanial and Immune Modulation of Trigonelline Against Leishmania major DOI Open Access
Elaheh Esmaeili, Ebrahim Saedi Dezaki, Hossein Amini-Khoei

и другие.

Parasite Immunology, Год журнала: 2024, Номер 46(12)

Опубликована: Дек. 1, 2024

The mechanistic study of new pharmaceutical compounds is crucial for evaluating their efficacy, identifying potential side effects, and optimising drug formulations. This aimed to investigate the mechanism action trigonelline on promastigote amastigote stages Leishmania major (MRHO/IR/75/ER). An initial in silico was conducted examine pharmacological effects using molecular docking evaluate binding affinity with nitrate, a molecule macrophage immune response against Leishmania. In this experimental study, inhibitory promastigotes evaluated by measuring metacaspase expression levels. stage L. major, levels inducible nitric oxide synthase (iNOS), interleukin 12 (IL-12), interferon-gamma (IFN-γ), tumour necrosis factor alpha (TNF-α), transforming growth factor-β (TGF-β) 10 (IL-10) genes were assessed Real-time PCR. Trigonelline demonstrated high-binding iNOS computer modelling. macrophages treated various concentrations trigonelline, glucantime combination, metacaspase, IL-12, TNF-α, IFN-γ significantly increased compared control group (p < 0.05), whereas IL-10 TGF-β gene decreased 0.05). exerts its antileishmanial through high antioxidant properties, non-cytotoxicity macrophages, ability enhance apoptosis cell cycle arrest major.

Язык: Английский

Metal Oxide–Enhanced Para-Coumaric Acid Nanoparticles for Precision Targeting of Leishmania donovani DOI
Kartikesh Sahu,

Raj Amrit Singh Samant,

Tiasha Routh

и другие.

BioNanoScience, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 7, 2025

Язык: Английский

Процитировано

1
Interfacing Reduced Graphene Oxide with Cationic Pillar[5]arene for Doxorubicin Delivery: A Platform for Glioblastoma Treatment DOI Creative Commons
Isabela A. A. Bessa, João Victor Roza Cruz,

Mikaelly O. B. Sousa

и другие.

ACS Applied Nano Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

0
Antiprotozoal Natural Products from Endophytic Fungi Associated with Cacao and Coffee DOI Creative Commons
Cristopher A. Boya P., Candelario Rodríguez, Randy Mojica-Flores

и другие.

Metabolites, Год журнала: 2024, Номер 14(11), С. 575 - 575

Опубликована: Окт. 25, 2024

Collectively, leishmaniasis and Chagas disease cause approximately 8 million cases more than 40,000 deaths annually, mostly in tropical subtropical regions. The current drugs used to treat these diseases have limitations many undesirable side effects; hence, new with better clinical profiles are needed. Fungal endophytes associated plants known produce a wide array of bioactive secondary metabolites, including antiprotozoal compounds. In this study, we analyzed endophytic fungal isolates

Язык: Английский

Процитировано

0
In Vitro Antileishmanial and Immune Modulation of Trigonelline Against Leishmania major DOI Open Access
Elaheh Esmaeili, Ebrahim Saedi Dezaki, Hossein Amini-Khoei

и другие.

Parasite Immunology, Год журнала: 2024, Номер 46(12)

Опубликована: Дек. 1, 2024

The mechanistic study of new pharmaceutical compounds is crucial for evaluating their efficacy, identifying potential side effects, and optimising drug formulations. This aimed to investigate the mechanism action trigonelline on promastigote amastigote stages Leishmania major (MRHO/IR/75/ER). An initial in silico was conducted examine pharmacological effects using molecular docking evaluate binding affinity with nitrate, a molecule macrophage immune response against Leishmania. In this experimental study, inhibitory promastigotes evaluated by measuring metacaspase expression levels. stage L. major, levels inducible nitric oxide synthase (iNOS), interleukin 12 (IL-12), interferon-gamma (IFN-γ), tumour necrosis factor alpha (TNF-α), transforming growth factor-β (TGF-β) 10 (IL-10) genes were assessed Real-time PCR. Trigonelline demonstrated high-binding iNOS computer modelling. macrophages treated various concentrations trigonelline, glucantime combination, metacaspase, IL-12, TNF-α, IFN-γ significantly increased compared control group (p < 0.05), whereas IL-10 TGF-β gene decreased 0.05). exerts its antileishmanial through high antioxidant properties, non-cytotoxicity macrophages, ability enhance apoptosis cell cycle arrest major.

Язык: Английский

Процитировано

0