Small-sized
graphite-coated
CeO
2
NPs
with
multi-defective
structures
and
desirable
oxidase-like
activity
were
synthesized
by
an
eco-friendly,
simple,
sustainable
method.
Advanced Optical Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 16, 2024
Abstract
Detection
of
colorimetric
signals
is
commonly
used
in
various
analytical
methods
and
for
testing
non‐laboratory
resource‐limited
settings.
The
performance
assays
largely
based
on
nanoparticles
their
unique
optical
properties.
Multifunctional
combining
enzyme‐like
catalytic
properties—known
as
nanozymes—hold
great
promise
applications
signal‐generating
labels.
However,
the
extensive
focus
properties
leaves
overlooked.
In
this
article,
use
nanozymes
reviewed
relying
inherent
nanozymes,
detection
a
catalytically‐formed
product,
changes
caused
by
product.
impact
extinction
coefficient
reaction
products,
well
kinetic
parameters
sensitivity
limit
assays,
are
quantitatively
evaluated.
Finally,
existing
limitations
prospects
biosensors
summarized.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 11, 2025
Natural
enzyme
cascades,
including
the
same
and
different
kinds
of
enzymes,
have
been
widely
used
in
biosensing.
However,
it
is
still
a
challenge
for
nanozymes,
mimics
natural
to
achieve
biosensing
with
types
enzyme-like
activities
based
on
cascade
reactions,
such
as
hydrolase
oxidoreductase.
Considering
characteristics
multienzyme-like
activity
some
proof
concept,
this
study
demonstrates
Cu/Ce-MOF-808
nanozyme
self-cascade
catalyst
hydrolase-like
catechol
oxidase-like
activities.
Taking
organophosphorus
pesticides
an
example,
we
constructed
catalytic
reaction
one-step
colorimetric
detection
isocarbophos.
Specifically,
can
hydrolyze
isocarbophos
produce
colorless
isopropyl
salicylate,
which
further
oxidized
by
complexed
4-aminoantipyrine
(4-AAP)
red
quinoneimine
under
neutral
conditions
illumination.
Thus,
strategy
sensitive
analysis
developed,
linear
range
0.90-400.00
μg·mL-1
limit
0.42
μg·mL-1.
This
research
presents
new
approach
design
synthesis
nanozymes
has
great
application
potential
field