Biomimetic bioreactor for potentiated uricase replacement therapy in hyperuricemia and gout

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 12

Опубликована: Янв. 7, 2025

Uricase replacement therapy is a promising approach for managing hyperuricemia and gout but hindered by challenges such as short blood circulation time, reduced catalytic activity, excessive hydrogen peroxide (H2O2) production. These limitations necessitate innovative strategies to enhance therapeutic efficacy safety. We designed synthesized RBC@SeMSN@Uri, red cell-coated biomimetic self-cascade bioreactor, which encapsulates uricase (Uri) selenium-based nano-scavenger (SeMSN) within RBC membranes. This design aims reduce immunogenicity, extend systemic circulation, maintain enzymatic activity. In vitro assays were conducted evaluate biocompatibility, anti-inflammatory effects, oxidative stress protection. vivo experiments in models assessed efficacy, biodistribution, biosafety. RBC@SeMSN@Uri effectively degraded uric acid (UA) into allantoin converted H2O2 water, preventing damage inflammation. demonstrated excellent biocompatibility H2O2-induced inflammatory responses compared free uricase. vivo, the bioreactor prolonged significantly levels, alleviated kidney damage, mitigated symptoms of gout. It also targeted inflamed joints, reducing swelling inflammation gouty arthritis models. study presents novel strategy enzyme By integrating membranes, addresses key traditional therapies, offering enhanced stability, superior efficacy. platform holds potential broader applications protein or antibody delivery therapies other diseases.

Язык: Английский

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One-step engineered mesenchymal stem cell-derived exosomes against hepatic ischemia–reperfusion injury

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125292 - 125292

Опубликована: Янв. 1, 2025

Язык: Английский

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Multifunctional Zwitterionic N‐Oxide Polymers to Overcome Cascade Physiological Barriers for Efficient Anticancer Drug Delivery

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Abstract For efficient anticancer drug delivery, cascade physiological barriers must be overcome, which requires the delivery vehicles to possess different or even opposite properties at stages. Poly(tertiary amine‐oxide) (PTAO) polymers with zwitterionic feature have distinct antifouling in blood circulation, can reduced and protonated hypoxic tumors promote cellular internalization. Nevertheless, effects of various PTAO structures not been studied systemically optimized. In this report, side groups are proposed optimized by modulating structures. Poly(2‐( N ‐oxide‐hexamethyleneimino)ethyl methacrylate) (POC7A) a cyclic seven‐membered ring is screened as structure for vivo applications. Moreover, block copolymer POC7A‐block‐poly( ε ‐caprolactone) (POC7A‐PCL) prepared formation micelles aqueous solution doxorubicin (DOX). The nature POC7A shells bioreductive activity protonation tumor microenvironment endows excellent long accumulation, deep penetration, effective Thus, DOX‐loaded exhibit potent antitumor efficacy after intravenous administration. Zwitterionic used nanocarriers overcome efficiently.

Язык: Английский

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Crystallization of supersaturated PEG-b-PLA for the production of drug-loaded polymeric micelles

Journal of Controlled Release, Год журнала: 2025, Номер 380, С. 457 - 468

Опубликована: Фев. 11, 2025

Язык: Английский

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Boosting the antibacterial potency of natural products through nanotechnologies

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125437 - 125437

Опубликована: Март 1, 2025

Язык: Английский

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Plant-Derived Flavonoid and Antibiotic Self-Assembled Nanomedicines for Enhanced Efficacy Against Bacterial Infections

ACS Applied Nano Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Язык: Английский

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Drug delivery under cover of erythrocytes extends drug half-life: A thrombolytic targeting therapy utilizing microenvironment-responsive artificial polysaccharide microvesicles

Carbohydrate Polymers, Год журнала: 2024, Номер 343, С. 122505 - 122505

Опубликована: Июль 20, 2024

Язык: Английский

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Material-based treatment strategies against intraosseous implant biofilm infection

Biochemistry and Biophysics Reports, Год журнала: 2024, Номер 39, С. 101764 - 101764

Опубликована: Июнь 30, 2024

Язык: Английский

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Impact of Zwitterions on the Acidity Constant and Glucose Sensitivity of Block Copolymers with Phenylboronic Acid

Langmuir, Год журнала: 2024, Номер 40(19), С. 10355 - 10361

Опубликована: Апрель 30, 2024

Molecular assemblies that transform in response to pH and saccharide concentration are promising nanomaterials the field of biomedicine, polymeric micelles amphiphilic polymers with phenylboronic acids (PBAs) have been studied. Herein, we report impact zwitterions on acidity constant for collapse glucose sensitivity a micelle produced from diblock copolymer comprising polyacrylamides PBA zwitterionic carboxybetaine (PAEBB-b-PCBAAm). The was synthesized through reversible addition–fragmentation chain-transfer polymerization followed by deprotection. PAEBB-b-PCBAAm micellar aggregates aqueous solutions at neutral pH, collapsed 11.0 because transformed into hydroxyboronate anion. pKa decreased presence owing boronate ester formation. PCBAAm chain significantly increased which molecular dissociated. This is probably boronic acid dipolar interaction zwitterions, and/or polymer corona valid screening ionization electrostatic repulsion anions. study modulation can facilitate design pH- saccharide-responsive biomaterials.

Язык: Английский

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One-Step On-Chip Preparation of Nanoparticle-Conjugated Red Blood Cell Carriers

Colloids and Surfaces B Biointerfaces, Год журнала: 2024, Номер 246, С. 114373 - 114373

Опубликована: Ноя. 14, 2024

Язык: Английский

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