Critical Reviews in Food Science and Nutrition,
Год журнала:
2024,
Номер
unknown, С. 1 - 19
Опубликована: Дек. 30, 2024
Multi-target
detection
technology
in
food
plays
a
key
role
the
field
of
analysis,
which
can
comprehensively
and
accurately
assess
levels
multiple
residues
food,
thus
ensuring
safety
quality.
In
recent
years,
smart
nanostructured
molecular
logic
technologies
(SNMLT)
have
been
widely
employed
multiplex
detection.
Of
these,
component
materials-based
SNMLT
attracted
much
attention
owing
to
their
rapid
response,
multifunctionality,
programmability,
high
sensitivity.
Nevertheless,
there
is
no
review
focusing
on
sensing
platform
for
multi-target
current
review,
principles
operation
classification
were
first
described,
followed
by
summary
materials
signal
output
strategies
SNMLT.
Besides,
applications
assay,
including
physical,
biological,
chemical
hazard
factors
quality
also
highlighted.
Finally,
future
challenges
summarized
discussed.
The
indicates
that
has
advantages
wide
range,
strong
anti-interference
ability,
ability
analyze
target
analytes
simultaneously
research
focus
combining
with
artificial
intelligence
develop
inexpensive
easy-to-use
devices.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 13, 2025
DNAzyme-based
cascade
networks
are
effective
tools
to
achieve
ultrasensitive
detection
of
low-abundance
miRNAs.
However,
their
designs
complicated
and
costly,
the
operation
is
time-consuming.
Herein,
a
novel
simple
noncascade
DNAzyme
network
designed
its
amplification
effect
comparable
or
even
better
than
many
cascading
ones.
It
nonenzymatic,
isothermal,
bicirculating
consisting
two
toehold-mediated
strand-displacement
reactions
localized
strategy.
Taking
microRNA-122
as
target
model,
this
fluorescence
biosensor
has
limit
84
zmol
L–1,
which
8-orders
magnitude
lower
that
nonamplification
one.
The
ultrasensitivity
mainly
benefits
from
exclusive
design
positive
self-feedback
mechanism
ingenious
network.
In
addition,
utilization
superparamagnetic
Fe3O4@SiO2
particles
not
only
helps
for
localization
DNAzymes
but
also
facilitates
rapid
separation
signal
probes
(output
DNA-CdTe
QDs).
This
fluorescent
advantages
specificity,
speed,
thermal
stability,
low
cost.
paves
new
way
bioamplification
strategy,
may
be
very
attractive
biosensors,
DNA
logic
gates,
computers.
Nucleic Acids Research,
Год журнала:
2024,
Номер
52(12), С. 7384 - 7396
Опубликована: Июнь 3, 2024
Abstract
The
revolutionary
technology
of
CRISPR/Cas
has
reshaped
the
landscape
molecular
biology
and
engineering.
This
tool
is
interest
to
researchers
in
multiple
fields,
including
diagnostics,
biochemistry
circuits,
information
storage.
As
spreads
more
niche
areas,
new
application
scenarios
requirements
emerge.
Developing
programmability
compatibility
becomes
a
critical
issue
phase.
Here,
we
report
redundancy-based
modular
CRISPR/Cas12a
synergistic
activation
platform
(MCSAP).
position,
length,
concentration
redundancy
split
DNA
activators
can
finely
regulate
activity
Cas12a.
With
redundant
structure
as
an
interface,
MCSAP
serves
plug-in
seamlessly
integrate
with
upstream
network.
successfully
performs
three
different
tasks:
nucleic
acid
detection,
enzyme
logic
operation.
work
effector
for
networks
because
its
programmability.
Our
provides
powerful
yet
easy-to-use
tools
strategies
fields
nanotechnology,
engineering,
biology.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 17, 2025
A
sensitive
fluorescence
biosensor
was
developed
for
microcystin-LR
(MC-LR)
detection
using
H1,
H2,
and
H3
DNA
probes
as
sensing
elements.
The
aptamer
in
H1
can
recognize
the
target.
H2
labeled
with
FAM
BHQ.
MC-LR
binding
will
activate
self-assemblies
through
toehold-mediated
strand
displacement.
In
formed
products
(MC-LR/H1/nH2/nH3),
BHQ
be
separated
a
high
signal
observed
assay.
is
limit
of
53
fM.
We
further
constructed
several
logic
circuits
(AND-AND
cascaded
circuit,
feedforward
resource
allocation
circuit)
MC-LR,
MC-LA,
MC-YR
three
inputs.
numbers
0
1
are
used
to
code
input
output
signals.
AND-AND
cascade
circuit
produce
only
(111)
combination.
MC-LA
signals,
inhibit
self-assembly
execute
negative
operation.
Through
rational
design
probe
hybridizations
on
four
different
magnetic
beads
(MBs),
achieve
an
intelligent
information.
Our
proposed
not
provide
platform
microcystin
but
also
serve
smart
system
sensing.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
A
newly
identified
hemin
aptamer
with
a
non-G-quadruplex
structure
exhibits
stronger
peroxidase
activity
and
selectivity
than
traditional
G-quadruplex/hemin
DNAzymes,
addressing
challenges
such
as
weak
binding,
low
catalytic
activity,
poor
selectivity.
In
this
study,
we
optimized
ion
activation
conditions,
refined
reaction
parameters,
developed
spontaneous
recombination
method
via
splitting
to
enhance
DNAzyme
enable
regulation.
The
demonstrated
superior
performance
in
enzyme-free
sensing,
polymerase-assisted
amplification,
CRISPR/Cas12a
systems,
achieving
higher
sensitivity
improved
colorimetric
thresholds
compared
G-quadruplexes.
We
have
also
comprehensive
operational
guide
for
aptamer/hemin
which
is
poised
revolutionize
sensor
signal
generation
elements.
DNA
storage
has
become
an
attractive
alternative
to
long-term,
stable
digital
data
because
of
its
high
density
and
strong
stability.
Recently,
numerous
efforts
have
been
made
develop
access
methods
improve
the
efficiency
accuracy
molecular
reading.
However,
most
current
were
achieved
by
well-developed
polymerase
chain
reaction
(PCR)
hybridization,
which
lack
exploration
dynamic
programmable
operations
for
access.
Here,
we
propose
a
strategy
in
nanoparticle
folders
are
controlled
DNAzyme
circuits
achieve
specific
information
manipulation.
We
experimentally
demonstrate
three
kinds
circuit
programs
that
YES,
AND,
OR
logic
manner.
In
addition,
selective
was
performed
using
obtain
target
from
pool.
Importantly,
extended
circuit-controlled
framework
multiple
manipulation
modes,
demonstrating
four
manipulations
on
two
AuNP
different
demand.
The
provides
paradigm
integrated
computing
systems
more
applications
fields
computation
storage.
Abstract
Modern
cryptography
based
on
computational
complexity
theory
is
mainly
constructed
with
silicon‐based
circuits.
As
DNA
nanotechnology
penetrates
the
molecular
domain,
utilizing
for
data
access
protection
in
biomolecular
domain
becomes
a
unique
approach
to
information
security.
However,
building
security
devices
and
strategies
robust
compatibility
still
challenging.
Here,
this
study
reports
time‐controlled
authentication
strategy
using
DNAzyme
strand
displacement
as
basic
framework.
A
time
limit
exists
authorization
access,
spontaneous
shutdown
design
further
protects
secure
access.
Multiple
hierarchical
authentications,
temporal
Boolean
logic
authentication,
enzyme
are
networks'good
programmability.
This
gives
proof
of
concept
detection
bioinformation
about
single
nucleotide
variants
miRNA,
highlighting
their
potential
biosensing
protection.
