Biomolecules,
Год журнала:
2024,
Номер
14(12), С. 1628 - 1628
Опубликована: Дек. 19, 2024
Due
to
their
high
biocompatibility,
biodegradability,
and
facile
surface
functionalization,
phospholipid
vesicles
as
carriers
have
garnered
significant
attention
in
the
realm
of
disease
diagnosis
treatment.
On
one
hand,
can
function
probes
for
detection
various
diseases
by
encapsulating
nanoparticles,
thereby
enabling
precise
localization
pathological
changes
monitoring
progression.
other
possess
capability
selectively
target
deliver
therapeutic
agents,
including
drug
molecules,
genes
immune
modulators,
affected
sites,
enhancing
sustained
release
these
agents
improving
efficacy.
Recent
advancements
nanotechnology
led
an
increased
focus
on
application
delivery,
biological
detection,
gene
therapy,
cell
mimics.
This
review
aims
provide
a
concise
overview
structure,
characteristics,
preparation
techniques
varying
sizes.
Furthermore,
we
will
summarize
latest
research
developments
regarding
use
nanomedicines
Additionally,
elucidate
potential
facilitating
internalization,
controlled
release,
targeted
delivery
substrates.
Through
this
review,
aspire
enhance
understanding
evolution
within
field,
outline
prospective
research,
address
forthcoming
challenges
associated
with
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 19, 2025
Precise
identification
and
analysis
of
multiple
protein
biomarkers
on
the
surface
breast
cancer
cell-derived
extracellular
vesicles
(BC-EVs)
are
great
significance
for
noninvasive
diagnosis
subtypes,
but
it
remains
a
major
challenge
owing
to
their
high
heterogeneity
low
abundance.
Herein,
we
established
CRISPR-based
homogeneous
electrochemical
strategy
near-zero
background
ultrasensitive
detection
BC-EVs.
To
realize
high-performance
capture
isolation
BC-EVs,
fluidity-enhanced
magnetic
nanoprobes
were
facilely
prepared.
After
capturing
AND
logic
gate-based
catalytic
hairpin
assembly
(CHA)
trans-cleavage
activity
CRISPR-Cas12a
against
signal
triggered
successively,
generating
significant
signal.
Notably,
as-developed
metal-mediated
could
efficiently
decrease
by
separation,
endowing
method
with
signal-to-noise
ratio.
Consequently,
ingeniously
integrating
DNA
CRISPR-CHA
amplification
dual
in
strategy,
precise
BC-EVs
was
successfully
achieved
through
simultaneous
specific
recognition
markers
surface.
More
importantly,
this
approach
effectively
discriminate
subgroups
clinical
serum
samples,
which
may
provide
opportunities
accurate
prognosis
evaluation
manner.
Abstract
Enhancing
the
sensitivity
of
biomedical
spectroscopy
is
crucial
for
advancing
medical
research
and
diagnostics.
Metasurfaces
have
emerged
as
powerful
platforms
enhancing
various
spectral
detection
technologies.
This
capability
arises
from
their
unparalleled
ability
to
improve
interactions
between
light
matter
through
localization
enhancement
fields.
In
this
article,
we
review
representative
approaches
recent
advances
in
metasurface-enhanced
spectroscopy.
We
provide
a
comprehensive
discussion
technologies
enhanced
by
metasurfaces,
including
infrared
spectroscopy,
Raman
fluorescence
other
modalities.
demonstrate
advantages
metasurfaces
improving
sensitivity,
reducing
limits,
achieving
rapid
biomolecule
while
discussing
challenges
associated
with
design,
preparation,
stability
procedures.
Finally,
explore
future
development
trends
biological
emphasize
wide-ranging
applications.
Aging and Disease,
Год журнала:
2025,
Номер
unknown, С. 0 - 0
Опубликована: Янв. 1, 2025
Nanozymes,
which
are
nanomaterials
that
replicate
the
catalytic
activities
of
natural
enzymes
in
biological
systems,
have
recently
demonstrated
considerable
potential
improving
cancer
immunotherapy
by
altering
tumor
microenvironment.
Nanozyme-driven
immune
responses
represent
an
innovative
therapeutic
modality
with
high
effectiveness
and
minimal
side
effects.
These
nanozymes
activate
system
to
specifically
recognize
destroy
cells.
Combined
immunotherapeutic
agents,
can
amplify
anti-cancer
integrating
remodeling
immunogenic
cell
death
(ICD).
This
review
offers
a
thorough
discussion
about
various
involved
immunity,
including
those
mimicking
catalase
(CAT),
superoxide
dismutase
(SOD),
peroxidase
(POD),
oxidase
(OXD).
It
also
discusses
challenges
future
directions
for
translating
nanozyme
platforms
into
clinical
applications,
enhancing
susceptibility
cells
immunotherapy.
Nanozyme-based
strategies
substantial
oncology,
offering
new
effective
options
management.
Abstract
Self‐powered
biosensors
(SPBs)
based
on
biofuel
cells
(BFCs)
use
electrical
output
as
a
sensing
signal
without
the
need
of
external
power
supplies,
providing
feasible
approach
to
constructing
miniaturized
implantable
or
portable
devices.
In
this
work,
novel
nanozyme
gold
nanoclusters/palladium
nanocubes
(AuNCs/PdNCs)
heterostructure
is
successfully
fabricated
develop
an
innovatively
self‐powered
and
non‐enzymatic
glucose
system.
The
AuNCs/PdNCs
with
oxidase
(GOD)‐like
activity
exhibits
superior
electrocatalytic
performance
toward
glucose.
BFCs‐based
SPBs
system,
established
(anodic
catalyst)
single
atomic
Fe
sites
coupled
carbon‐encapsulated
3
C
crystals
(Fe
C@C‐Fe
SACs
cathodic
platform,
exceptional
sensitivity
0.151
µW
cm
−2
m
−1
(3.4
times
higher
than
PdNCs),
outstanding
selectivity
robust
stability.
system
can
be
attributed
synergistic
cooperation
between
PdNCs
AuNCs.
Colorectal
cancer
(CRC)
remains
a
formidable
threat
to
human
health,
with
considerable
challenges
persisting
in
its
diagnosis,
particularly
during
the
early
stages
of
malignancy.
In
this
study,
we
elucidated
that
fecal
extracellular
vesicle
microRNA
signatures
(FEVOR)
could
serve
as
potent
noninvasive
CRC
biomarkers.
FEVOR
was
first
revealed
by
miRNA
sequencing,
followed
construction
CRISPR/Cas13a-based
detection
platform
interrogate
expression
across
diverse
spectrum
clinical
cohorts.
Machine
learning-driven
models
were
subsequently
developed
within
realms
diagnostics,
prognostics,
and
warning
systems.
cohort
38
patients,
our
diagnostic
model
achieved
an
outstanding
accuracy
97.4%
(37/38),
successfully
identifying
37
cases.
This
performance
significantly
outpaced
efficacy
two
clinically
established
biomarkers,
CEA
CA19-9,
which
showed
accuracies
mere
26.3%
(10/38)
7.9%
(3/38),
respectively.
We
also
examined
levels
several
patients
both
before
after
surgery,
well
colorectal
adenomas
(CA).
Impressively,
results
robust
prognostic
indicator
for
potential
predictor
CA.
endeavor
aimed
harness
predictive
power
enhancing
precision
management
paradigms.
envision
these
findings
will
propel
foundational
preclinical
research
on
CRC,
studies.
