Beta-Barrel Nanopores as Diagnostic Sensors: An Engineering Perspective DOI Creative Commons

Rani Wiswedel,

Anh Bui,

Jin-Hyung Kim

и другие.

Biosensors, Год журнала: 2024, Номер 14(7), С. 345 - 345

Опубликована: Июль 16, 2024

Biological nanopores are ultrasensitive and highly attractive platforms for disease diagnostics, including the sequencing of viral microbial genes detection biomarkers pathogens. To utilize biological as diagnostic sensors, they have been engineered through various methods resulting in accurate sensitive disease-related biomolecules. Among diverse nanopores, β-barrel-containing advantages nanopore engineering because their robust structure, making them well-suited modifications. In this review, we highlight approaches used single-molecule sensing applications early diagnosis prognosis. highlighted studies, β-barrel can be modified by genetic mutation to change structure; alter charge distributions; or add enzymes, aptamers, protein probes enhance sensitivity accuracy. Furthermore, review discusses challenges future perspectives advancing nanopore-based sensors.

Язык: Английский

DNAzyme-Assisted the Detection of rps27l mRNA in Protein Nanopores DOI
Kang Luo, Xu Wang,

Ling Zheng

и другие.

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 343711 - 343711

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Highly Sensitive and Interference-Free Detection of Multiple Drug Molecules in Serum Using Dual-Modified SERS Substrates Combined with AI Algorithm Analysis DOI
Wang Ying-ji, Jin Sun, Liping Zhou

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 3, 2025

Surface-enhanced Raman spectroscopy (SERS) technology has shown broad potential in drug concentration detection, but its application blood monitoring faces significant challenges. The primary difficulty lies overcoming the interference caused by various biomolecules present serum, which can severely obscure SERS signals of target molecules. Traditional enhancement substrates are often limited to detecting single drugs and prone interference, making label-free detection multiple particularly challenging. To address these issues, we developed a novel substrate based on Au@AgNRs, undergoes two-step modification produce Au@AgDBCNRs. This innovative provides exceptional signal amplification, simultaneously allowing sensitive Moreover, our method eliminates need for serum deproteinization, enabling direct while effectively mitigating from components. cetyltrimethylammonium bromide coating Au@AgDBCNRs is an internal standard quantification without additional standards. platform significantly improves accuracy efficiency automatically integrating artificial intelligence recognize analyze spectral features. new idea therapeutic expected provide rapid, accurate, cost-effective clinical environment, great improving patient care optimizing dosage strategies.

Язык: Английский

Процитировано

0

Pore lipid modifications modulate MscS nanopore for enhanced single-molecule sensing DOI
Changjian Zhao,

Xingyu Mou,

Qianqian Zhang

и другие.

Biosensors and Bioelectronics, Год журнала: 2025, Номер 276, С. 117250 - 117250

Опубликована: Фев. 11, 2025

Язык: Английский

Процитировано

0

Nanopore Environmental Analysis DOI Creative Commons
Xiaofeng Lu, Xiaoyu Du, Dong Zhong

и другие.

JACS Au, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Beta-Barrel Nanopores as Diagnostic Sensors: An Engineering Perspective DOI Creative Commons

Rani Wiswedel,

Anh Bui,

Jin-Hyung Kim

и другие.

Biosensors, Год журнала: 2024, Номер 14(7), С. 345 - 345

Опубликована: Июль 16, 2024

Biological nanopores are ultrasensitive and highly attractive platforms for disease diagnostics, including the sequencing of viral microbial genes detection biomarkers pathogens. To utilize biological as diagnostic sensors, they have been engineered through various methods resulting in accurate sensitive disease-related biomolecules. Among diverse nanopores, β-barrel-containing advantages nanopore engineering because their robust structure, making them well-suited modifications. In this review, we highlight approaches used single-molecule sensing applications early diagnosis prognosis. highlighted studies, β-barrel can be modified by genetic mutation to change structure; alter charge distributions; or add enzymes, aptamers, protein probes enhance sensitivity accuracy. Furthermore, review discusses challenges future perspectives advancing nanopore-based sensors.

Язык: Английский

Процитировано

2