Nanosensor-based Imaging of Realtime Dopamine Release in Neurons Derived from iPSCs of Patients with Parkinson’s Disease DOI Creative Commons
Nayeon Lee, Dakyeon Lee,

Jae Hyeok Lee

и другие.

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101485 - 101485

Опубликована: Янв. 19, 2025

Dopamine (DA) is an essential neuromodulator that underlies critical aspects of cognitive processes, motor function, and reward systems. Disruptions in DA signaling contribute to various neurodegenerative diseases, including Parkinson's disease (PD). Despite its important role neuronal the impact release/uptake on neurochemical imbalances during development remains unclear. We propose a novel application near-infrared catecholamine nanosensor (NIRCat) for real-time visualization neurotransmission among cells. The fluorescence (900-1400 nm) NIRCat allows semi-quantitative measurement release living neurons offers insights into cellular dynamics neuropathological development. In this study, we applied elucidate human induced pluripotent stem cells (hiPSCs)-derived dopaminergic from both healthy control PD patient carrying GBA1 mutations. accurately quantified electrically stimulated events, identifying distinct 'hotspots' activity across Our findings present significantly enhanced spatial temporal resolution signaling, providing deeper understanding balance progression disease.

Язык: Английский

Nanosensor-based Imaging of Realtime Dopamine Release in Neurons Derived from iPSCs of Patients with Parkinson’s Disease DOI Creative Commons
Nayeon Lee, Dakyeon Lee,

Jae Hyeok Lee

и другие.

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101485 - 101485

Опубликована: Янв. 19, 2025

Dopamine (DA) is an essential neuromodulator that underlies critical aspects of cognitive processes, motor function, and reward systems. Disruptions in DA signaling contribute to various neurodegenerative diseases, including Parkinson's disease (PD). Despite its important role neuronal the impact release/uptake on neurochemical imbalances during development remains unclear. We propose a novel application near-infrared catecholamine nanosensor (NIRCat) for real-time visualization neurotransmission among cells. The fluorescence (900-1400 nm) NIRCat allows semi-quantitative measurement release living neurons offers insights into cellular dynamics neuropathological development. In this study, we applied elucidate human induced pluripotent stem cells (hiPSCs)-derived dopaminergic from both healthy control PD patient carrying GBA1 mutations. accurately quantified electrically stimulated events, identifying distinct 'hotspots' activity across Our findings present significantly enhanced spatial temporal resolution signaling, providing deeper understanding balance progression disease.

Язык: Английский

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