BOOST: a robust ten-fold expansion method on hour-scale DOI Creative Commons

JinYu Guo,

Hui Yang, Chixiang Lu

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 2, 2025

Expansion microscopy enhances the resolution by physically expanding biological specimens and improves visualization of structural molecular details. Numerous expansion techniques labeling methods have been developed over past decade to cater specific research needs. Nonetheless, a shared limitation among current protocols is extensive sample processing time, particularly for challenging-to-expand (e.g., formalin-fixed paraffin-embedded (FFPE) sections large three-dimensional specimens). Here we present BOOST, rapid robust workflow that leverages series microwave-accelerated chemistry. Specifically, BOOST enables single-step 10-fold cultured cells, tissue sections, even FFPE under 90 minutes. Notably, pioneers millimeter-sized specimens, previously unattainable best our knowledge. The also easily adaptable based on stable common reagents, thus boosting potential adoption applications.

Язык: Английский

BOOST: a robust ten-fold expansion method on hour-scale DOI Creative Commons

JinYu Guo,

Hui Yang, Chixiang Lu

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 2, 2025

Expansion microscopy enhances the resolution by physically expanding biological specimens and improves visualization of structural molecular details. Numerous expansion techniques labeling methods have been developed over past decade to cater specific research needs. Nonetheless, a shared limitation among current protocols is extensive sample processing time, particularly for challenging-to-expand (e.g., formalin-fixed paraffin-embedded (FFPE) sections large three-dimensional specimens). Here we present BOOST, rapid robust workflow that leverages series microwave-accelerated chemistry. Specifically, BOOST enables single-step 10-fold cultured cells, tissue sections, even FFPE under 90 minutes. Notably, pioneers millimeter-sized specimens, previously unattainable best our knowledge. The also easily adaptable based on stable common reagents, thus boosting potential adoption applications.

Язык: Английский

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