Zinc deficiency causes oxidative stress, endoplasmic reticulum stress, apoptosis and inflammation in hepatocytes in grass carp

Fish & Shellfish Immunology, Год журнала: 2023, Номер 139, С. 108905 - 108905

Опубликована: Июнь 21, 2023

Язык: Английский

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Acute kidney injury: exploring endoplasmic reticulum stress-mediated cell death

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Фев. 12, 2024

Acute kidney injury (AKI) is a global health problem, given its substantial morbidity and mortality rates. A better understanding of the mechanisms factors contributing to AKI has potential guide interventions aimed at mitigating risk subsequent unfavorable outcomes. Endoplasmic reticulum stress (ERS) an intrinsic protective mechanism against external stressors. ERS occurs when endoplasmic (ER) cannot deal with accumulated misfolded proteins completely. Excess can eventually cause pathological reactions, triggering various programmed cell death (autophagy, ferroptosis, apoptosis, pyroptosis). This article provides overview latest research progress in deciphering interaction between different death. Additionally, report consolidates insights into roles highlights avenues for targeting as treatment direction toward AKI.

Язык: Английский

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Hinokitiol Prevents Diabetic Acute Kidney Injury by Mitigating ER Stress

Cell Biology International, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

ABSTRACT Acute kidney injury (AKI) in diabetic conditions often advances to chronic disease (CKD), exacerbated by ischemia–reperfusion (IRI) through pathomechanisms such as endoplasmic reticulum (ER) stress and inflammation. Currently, available treatment options for AKI are not uniformly effective, highlighting the need novel interventions. This study aimed examine renoprotective effects of hinokitiol, a natural tropolone compound, against with its capability decrease ER inflammation, along apoptosis. involved NRK‐52E cells grown in‐vitro under high‐glucose subjected 10 mM sodium azide elicit hypoxia/reperfusion (HRI). The expression key markers like binding immunoglobulin protein (BiP), R/PKR‐like kinase (PERK), eukaryotic initiation factor‐2 (eIF2α) well inflammatory proteins was markedly diminished hinokitiol pretreatment (50 μM). Hinokitiol further reduced apoptosis cells. Similarly, in‐vivo study, male Wistar rats STZ‐induced Type 1 diabetes (55 mg/kg, i.p .) were treated 50 100 mg/kg/day . 5 days, followed induction via bilateral IRI. significantly elevated plasma blood urea nitrogen (BUN), creatinine, urinary molecule‐1 (KIM‐1) levels tubular damage rats. also respective expressions rats, demonstrated immunohistochemical analysis immunoblotting. These findings suggest that alleviates modulating PERK/CHOP/NF‐κB axis, likeliness viable therapeutic technique alleviating AKI.

Язык: Английский

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Advantages and drawbacks of dexamethasone in glioblastoma multiforme

Critical Reviews in Oncology/Hematology, Год журнала: 2022, Номер 172, С. 103625 - 103625

Опубликована: Фев. 11, 2022

Язык: Английский

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Research Progress on Extraction, Isolation, Structural Analysis and Biological Activity of Polysaccharides from Panax Genus

Molecules, Год журнала: 2023, Номер 28(9), С. 3733 - 3733

Опубликована: Апрель 26, 2023

The panax genus is a widely used medicinal plant with good biological activity. As one of the main active components Panax genus, polysaccharides have various pharmacological effects. This review summarizes latest research reports on ginseng, American and notoginseng compares differences in extraction, isolation purification, structural characteristics, activities. current mainly focuses ginseng polysaccharides, process isolation, structure analysis each polysaccharide roughly same. Modern studies shown that these antioxidants, antitumor, immunomodulatory, antidiabetic, intestinal protection, skin repair, other provides new insights into between three kinds which will help to further study value traditional Chinese medicine.

Язык: Английский

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Comparisons of Isolation Methods, Structural Features, and Bioactivities of the Polysaccharides from Three Common Panax Species: A Review of Recent Progress

Molecules, Год журнала: 2021, Номер 26(16), С. 4997 - 4997

Опубликована: Авг. 18, 2021

Panax spp. (Araliaceae family) are widely used medicinal plants and they mainly include ginseng C.A. Meyer, quinquefolium L. (American ginseng), notoginseng (notoginseng). Polysaccharides the main active ingredients in these have demonstrated diverse pharmacological functions, but comparisons of isolation methods, structural features, bioactivities polysaccharides not yet been reported. This review summarizes recent advances associated with 112 from ginseng, 25 American 36 it compares differences extraction, purification, bioactivities. Most studies focus on typically made notoginseng. For analysis, processes similar for three species. Previous determined that 55 18 9 exhibited anti-tumor activity, immunoregulatory effects, anti-oxidant other which mediated by multiple signaling pathways, including mitogen-activated protein kinase, nuclear factor kappa B, or redox balance pathways. can provide new insights into similarities among species, facilitate guide further to explore properties Araliaceae family traditional Chinese medicine.

Язык: Английский

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Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury

Molecular Medicine, Год журнала: 2023, Номер 29(1)

Опубликована: Окт. 24, 2023

It is widely acknowledged that cisplatin-induced nephrotoxicity hinders its efficacy during clinical therapy. Effective pharmaceutical interventions for acute kidney injury (Cis-AKI) are currently lacking. Prior studies have implicated the chemokine CX3CL1 in development of lipopolysaccharide-induced AKI; however, specific role Cis-AKI remains uncertain. This research aimed to comprehensively characterize therapeutic impact and mechanism inhibition on Cis-AKI.This study employed an vivo mouse model vitro cisplatin-treated podocytes. Kidney pathological changes were assessed using hematoxylin-eosin (HE) Periodic-Schiff (PAS) staining. Transcriptome tissue post-cisplatin treatment analyzed through RNA sequencing (RNA-seq) datasets. Evaluation parameters included expression inflammatory markers, intracellular free iron levels, ferroptosis-related proteins-solute carrier family 7 member 11 (SLC7A11/XCT) glutathione peroxidase 4 (GPX4)-as well as lipid peroxidation markers mitochondrial function proteins. Mitochondrial morphological visualized transmission electron microscopy. The glucose-regulated protein 78/eukaryotic translation initiation factor 2A/CCAAT enhancer binding protein-homologous (GRP78/eIF2α/CHOP) hypoxia-inducible 1-alpha/heme oxygenase-1 (HIF1A/HO-1) pathways was via Western Blot Immunofluorescence experiments, both vitro.Kidney levels elevated following cisplatin injection wild-type (WT) mice. Cisplatin-treated CX3CL1-Knockout mice exhibited reduced renal histological changes, lowered blood creatinine (Cre) urea nitrogen (BUN) decreased mediators compared WT RNA-seq analysis revealed modulation associated with oxidative stress metabolism related ferroptosis kidneys Cis-AKI. Both podocytes demonstrated could mitigate ferroptosis. effect characterized by alleviated overload, malondialdehyde (MDA) content, reactive oxygen species (ROS) production, alongside increased glutathione/glutathione disulfide ratio, superoxide dismutase (SOD), XCT, GPX4 activity. also ameliorated dysfunction upregulated biogenesis proteins-uncoupling (UCP), mitofusin 2 (Mfn2), peroxisome proliferators-activated receptor γ coactivator l-alpha (PGC1α)-both vitro. Furthermore, attenuated endoplasmic reticulum (ER) Notably, HIF-1α HO-1 vitro.Our findings suggest exerts effects against suppressing podocyte

Язык: Английский

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Protective effect of spore oil-functionalized nano-selenium system on cisplatin-induced nephrotoxicity by regulating oxidative stress-mediated pathways and activating immune response

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Фев. 9, 2023

In clinical practice, cisplatin is the most commonly used chemotherapy drug to treat a range of malignancies. Severe ROS-regulated nephrotoxicity, however, restricts its applicability. Currently, main mechanisms leading cisplatin-induced nephrotoxicity in settings involve hydration or diuresis. However, not all patients can be treated with massive diuretics. Therefore, it crucial develop treatment modality that effectively reduce through foodborne route. Selenium has been reported have strong antioxidant as well anticancer effects when administered spore oil. Herein, we established cellular and animal models synthesized oil-functionalized nano-selenium (GLSO@SeNPs). We found GLSO@SeNPs inhibit mitochondrial apoptotic pathway by maintaining oxidative homeostasis regulating related signaling pathways (the MAPK, caspase, AKT pathways). vivo, could improve renal impairment, tissues thus inhibiting process injury. addition, were converted into selenocysteine (SeCys2), which may exert protective effects. Furthermore, modulate ratio immune cells kidneys spleen, reducing proportions CD3+CD4+ T cells, CD3+CD8+ M1 phenotype macrophages increasing proportion anti-inflammatory regulatory cells. summary, this study, food-derived nanomaterials, explored nephrotoxicity. Our study provides basis rationale for inhibition nutrients.

Язык: Английский

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Restoring cellular calcium homeostasis to rescue ER stress by 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid acetoxymethyl ester-loaded lipid-mPLGA hybrid-nanoparticles for acute kidney injury therapy

Chinese Chemical Letters, Год журнала: 2023, Номер 35(3), С. 108434 - 108434

Опубликована: Апрель 7, 2023

Язык: Английский

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Ultrasound-Assisted Extraction of Atractylodes chinensis (DC.) Koidz. Polysaccharides and the Synergistic Antigastric Cancer Effect in Combination with Oxaliplatin

ACS Omega, Год журнала: 2024, Номер 9(16), С. 18375 - 18384

Опубликована: Апрель 14, 2024

Oxaliplatin (OXA) is recognized as a first-line drug for gastric cancer. However, low accumulation of the OXA in target site and development resistance directly led to treatment failure. In present study, an ultrasonic extraction method Atractylodes chinensis (DC.) Koidz. polysaccharides (AKUs) combination with vitro were studied. Results showed that when pH level was 11, ultrasound power at 450 W, solid–liquid ratio 1:20, 30 min, yield AKUs significantly increased 13.20 ± 0.35%. The molecular weights ranged from 7.21 185.94 kDa, its monosaccharides mainly composed arabinose (Ara), galactose (Gal), glucose (Glu) 58.36, 16.90, 15.49%, respectively. Cell experiments that, compared alone (2 μg/mL, inhibition rate 18%), had significant synergistic inhibitory effect on MKN45 proliferation, which 33, 41, 45% increasing concentrations (5–50 μg/mL), respectively, representing 2.5-fold inhibition. Inductively coupled plasma-mass spectrometry (ICP-MS) determination confirmed intracellular uptake by 29%, alone. We first demonstrated combined cancer cells mediated reducing expression efflux proteins (MRP1 MRP2) ingested protein (OCT2). As result above, deserved be effective adjuvant chemotherapeutics clinical setting.

Язык: Английский

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