Application of network pharmacology in synergistic action of Chinese herbal compounds

Theory in Biosciences, Год журнала: 2024, Номер 143(3), С. 195 - 203

Опубликована: Июнь 18, 2024

Язык: Английский

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Biological significance and pathophysiological role of Matrix Metalloproteinases in the Central Nervous System

International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 135967 - 135967

Опубликована: Сен. 1, 2024

Язык: Английский

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Investigating compounds from Basella alba for their antioxidant, anti-inflammatory, and anticancer properties through in vitro and network pharmacology, molecular simulation approach

Green Chemistry Letters and Reviews, Год журнала: 2025, Номер 18(1)

Опубликована: Март 28, 2025

Язык: Английский

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System Biology Investigation Revealed Lipopolysaccharide and Alcohol-Induced Hepatocellular Carcinoma Resembled Hepatitis B Virus Immunobiology and Pathogenesis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(13), С. 11146 - 11146

Опубликована: Июль 6, 2023

Hepatitis B infection caused by the hepatitis virus is a life-threatening cause of liver fibrosis, cirrhosis, and hepatocellular carcinoma. Researchers have produced multiple in vivo models for (HBV) and, currently, there are no specific laboratory animal available to study HBV pathogenesis or immune response; nonetheless, their limitations prevent them from being used pathogenesis, response, therapeutic methods because can only infect humans chimpanzees. The current first its kind identify suitable chemically induced cirrhosis/HCC model that parallels pathophysiology. Initially, data peer-reviewed literature GeneCards database were compiled genes seven drugs (acetaminophen, isoniazid, alcohol, D-galactosamine, lipopolysaccharide, thioacetamide, rifampicin) regulate. Functional enrichment analysis was performed STRING server. network HBV/Chemical, genes, pathways constructed Cytoscape 3.6.1. About 1546 modulated HBV, which 25.2% 17.6% common alcohol lipopolysaccharide-induced hepatitis. In accordance with analysis, activates signaling apoptosis, cell cycle, PI3K-Akt, TNF, JAK-STAT, MAPK, chemokines, NF-kappa B, TGF-beta. addition, lipopolysaccharide significantly activated these more than other chemicals, higher gene counts lower FDR scores. conclusion, alcohol-induced could be chronic an acute inflammatory response HBV.

Язык: Английский

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Preparation and Optimization of Gemcitabine Loaded PLGA Nanoparticle Using Box-Behnken Design for Targeting to Brain: In Vitro Characterization, Cytotoxicity and Apoptosis Study

Current Nanomaterials, Год журнала: 2023, Номер 9(4), С. 324 - 338

Опубликована: Дек. 27, 2023

Background: Treatment of glioma with conventional approaches remains a far-reaching target to provide the desired outcome. This study aimed develop and optimize Gemcitabine hydrochloride- loaded PLGA nanoparticles (GNPs) using Box-Behnken design methodology. The independent variables chosen for this included quantity Polymer (PLGA) (X1), Tween 80 (X2), Sonication time (X3), whereas dependent were Particle size (Y1) EE % (Y2) PDI (Y3). optimized biodegradable investigated their anticancer effectiveness in U87MG human glioblastoma cells vitro. Method: formulation process involved two steps. Initially, emulsification was carried out by combining organic polymer solution aqueous surfactant solution. Subsequently, second step, solvent evaporated, resulting precipitation formation nanoparticles. PLGA, 80, PVA (at constant concentration) adjusted based on experimental trial approach. PLGA-based underwent characterization, wherein particle size, encapsulation efficiency, polydispersity index (PDI), cumulative release assessed. optimal composition determined as 200 mg 4 ml 2 PVA. Further, GNPs evaluated anti-cancer U87 MG MTT apoptosis assay. Results: results demonstrated that exhibited an efficiency 81.66 %, 140.1 nm, 0.37. morphology Opt-GNPs observed be spherical through transmission electron microscopy (TEM). Conclusion: Apoptosis further confirmed observations assay Opt- significantly enhanced U-87 than Standard marketed formulation.

Язык: Английский

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System biology approach to identify the novel biomarkers in glioblastoma multiforme tumors by using computational analysis

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Май 22, 2024

Introduction: The most common primary brain tumor in adults is glioblastoma multiforme (GBM), accounting for 45.2% of all cases. characteristics GBM, a highly aggressive tumor, include rapid cell division and propensity necrosis. Regretfully, the prognosis extremely poor, with only 5.5% patients surviving after diagnosis. Methodology: To eradicate these kinds complicated diseases, significant focus placed on developing more effective drugs pinpointing precise pharmacological targets. Finding appropriate biomarkers drug discovery entails considering variety factors, including illness states, gene expression levels, interactions between proteins. Using statistical techniques like p-values false rates, we identified differentially expressed genes (DEGs) as first step our research identifying promising GBM. Of 132 genes, 13 showed upregulation, 29 unique downregulation. No statistically changes remaining were observed. Results: Matrix metallopeptidase 9 (MMP9) had greatest degree hub biomarker identification, followed by (periostin (POSTN) at 11 Hes family BHLH transcription factor 5 (HES5) 9. significance identification each initiation advancement was brought to light survival analysis. Many participate signaling networks function extracellular areas, demonstrated enrichment analysis.We also factors kinases that control proteins proteinprotein (PPIs) DEGs. Discussion: We discovered connected every biomarker. It an appealing therapeutic target inhibiting MMP9 involved Molecular docking investigations indicated chosen complexes (carmustine, lomustine, marimastat, temozolomide) high binding affinities −6.3, −7.4, −7.7, −8.7 kcal/mol, respectively, mean root-mean-square deviation (RMSD) value carmustine complex marimastat 4.2 Å 4.9 Å, lomustine temozolomide system average RMSD 1.2 1.6 respectively. Additionally, stability fluctuation (RMSF) analysis observed no structural conformational among atomic molecules. Thus, silico develop new way experimentalists lethal diseases future.

Язык: Английский

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Role of Circular RNA MMP9 in Glioblastoma Progression: From Interaction With hnRNPC and hnRNPA1 to Affecting the Expression of BIRC5 by Sequestering miR‐149

Journal of Molecular Recognition, Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

ABSTRACT Glioblastoma multiforme (GBM) presents a significant challenge in neuro‐oncology due to its aggressive behavior and self‐renewal capacity. Circular RNAs (circRNAs), subset of non‐coding (ncRNAs) generated through mRNA back‐splicing, are gaining attention as potential targets for GBM research. In our study, we sought explore the functional role circMMP9 (circular form matrix metalloproteinase‐9) promising therapeutic target bioinformatic predictions human data analysis. Our results suggest that functions sponge miR‐149 miR‐542, both upregulated based on microarray data. Kaplan–Meier analysis indicated reduced levels miR‐542 correlate with worse survival outcomes GBM, suggesting their tumor suppressors. Importantly, has been demonstrated inhibit expression BIRC5 (baculoviral inhibitor apoptosis repeat‐containing 5 or survivin), promoter proliferation GBM. is not only but also various other cancers, including neuroblastoma brain cancers. protein–protein interaction highlights significance central hub gene CircMMP9 seems influence this complex relationship by suppressing despite increased Additionally, found directly interacts heterogeneous nuclear ribonucleoproteins C A1 (hnRNPC A1), although within protein‐binding domains. This suggests hnRNPC/A1 may play transporting circMMP9. Moreover, RNA‐seq from patient samples confirmed BIRC5, PIK3CB, hnRNPC/A1, further emphasizing propose first time new epigenetic regulatory circMMP9, highlighting novel aspect oncogenic function. regulate sponging miR‐542. turn, suppresses enhances Nonetheless, more extensive studies advised delve deeper into roles especially context glioma.

Язык: Английский

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Circular RNA MMP9 interacts with HNRNPC and HRNPA1 and potentially influences the expression of BIRC5 by sequestering miR-149 and supporting glioblastoma progress

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Май 7, 2024

Background Glioblastoma multiforme (GBM) presents a significant challenge in neuro-oncology due to its aggressive behavior and self-renewal capacity. Circular RNAs (circRNAs), subset of long non-coding (ncRNAs) generated through mRNA back-splicing, are gaining attention as potential targets for GBM research. In our study, we sought explore the functional role circMMP9 (circular form matrix metalloproteinase-9) promising therapeutic target bioinformatic predictions NGS data analysis. Results Our results suggest that functions sponge miR-149 miR-542, both which show upregulation based on microarray Kaplan-Meier analysis indicated reduced levels miR-542 correlate with worse survival outcomes GBM, suggesting their tumor suppressors. Importantly, has been demonstrated inhibit expression BIRC5 (baculoviral inhibitor apoptosis repeat-containing 5, also known survivin), promoter proliferation GBM. is not only upregulated but various other cancers, including neuroblastoma brain cancers. protein-protein interaction highlights significance central hub gene CircMMP9 seems influence this complex relationship by suppressing despite increased Additionally, found directly interacts HNRNPC (heterogeneous nuclear ribonucleoprotein C) HRNPA1 A1), although within protein-binding domains. This suggests may play transporting circMMP9. Moreover, RNA-seq from patient samples confirmed BIRC5, PIK3CB, HNRNPC, HRNPA1, further emphasizing Conclusion regulate sponging miR-542. turn, suppresses enhances Nonetheless, more extensive studies advised delve deeper into roles circMMP9, especially context glioma.

Язык: Английский

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