Liangxue Qushi Zhiyang Decoction Inhibits Atopic Dermatitis in Mice via FcγR‐Mediated Phagocytosis DOI Creative Commons
Lili Zhang, Linxian Li, Zhanxue Sun

и другие.

Mediators of Inflammation, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Background: Liangxue Qushi Zhiyang Decoction (LQZ) is a traditional formula known for its efficacy in treating Atopic Dermatitis (AD). However, the specific mechanisms through which LQZ alleviates AD symptoms remain largely unknown. The objective of this study to investigate protective effects on and uncover potential action. Methods: An model was established mice using 2,4‐dinitrochlorobenzene (DNCB). Mice were then orally administered or prednisolone (PDN). Throughout treatment period, dermatitis scores scratching frequencies regularly monitored. Histopathological analyses conducted hematoxylin eosin (H&E) staining toluidine blue (TB) staining. Serum levels inflammatory cytokines measured enzyme‐linked immunosorbent assay (ELISA). Further, tandem mass tag (TMT) labeling quantitative proteomics employed identify differentially expressed proteins (DEPs). Enrichment analysis pinpoint targets pathways involved LQZ’s therapeutic Finally, validation experiments performed further explore core treatment.. Results: notably mitigated skin barrier damage response induced by DNCB mice, reduced serum IgE, IL‐4, IL‐1 β . Proteomic identified 248 with differential expression, implicating multiple LQZ’ Among these, Fc gamma R(Fc γ R)−mediated phagocytosis pathway emerged as crucial factor AD’s immune responses. Key associated pathway, including Fc‐gamma RIII (Fcgr3), V‐yes‐1 Yamaguchi sarcoma viral related oncogene homolog (Lyn), Tyrosine‐protein kinase (Syk), Phosphoinositide phospholipase C‐gamma‐2 (Plcg2), Neutrophil cytosol 1 (Ncf1), Ras‐related C3 botulinum toxin substrate 2 (Rac2) Actin‐related protein 2/3 complex subunit 3 (Arpc3), exhibited significantly expression following treatment. Conclusion: effective alleviating reactions. Its anti‐AD properties may be attributed inhibition R‐mediated phagocytic pathway.

Язык: Английский

Separable Microneedle Patch Integrated with the Dictamnine-Loaded Copper MOF Nanozyme for Atopic Dermatitis Treatment DOI
Shuyun Liu, Rui Xin, Xinyue Zhang

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

Atopic dermatitis (AD) is a chronic inflammatory skin disorder marked by thickening, severe pruritus, lesions, and emotional disturbances, including anxiety depression-like behavior. Current treatments primarily rely on localized therapies, which can lead to adverse effects such as hyperglycemia Cushing's syndrome with repeated use. To address these issues, we developed hyaluronic acid-based separable microneedle patch (Dic@pCu-HA MN), integrating polydopamine-coordinated copper-based metal-organic frameworks (pCu-MOFs) the anti-inflammatory agent dictamnine (Dic), for synergistic management of AD its neuropsychiatric comorbidities. pCu-MOFs exhibited dual functionality nanocargo hydrophobic Dic (encapsulation efficiency: 84.62 ± 2.14%) multienzyme mimics that efficiently scavenge reactive oxygen species (ROS) (superoxide radical scavenging: 63.85 0.34%). In vitro release studies demonstrated ROS-responsive 86.80 4.83% over 48 h under pathology-mimicking conditions. 1-Chloro-2,4-dinitrochlorobenzene (DNCB)-induced mouse model, Dic@pCu-HA MN significantly reduced oxidative stress (8-OHdG: 85.1 7.0% decrease), suppressed pro-inflammatory cytokines (IL-4: 70.0 7.8% decrease vs control), restored barrier integrity. By modulating HPA axis, system attenuated neuroinflammation alleviated itching (scratching frequency: 40.1 41.3% reduction) behavior (time in bright box: 96.6 156.2% increase). This combined therapeutic approach not only offers comprehensive strategy but also provides potential benefits addressing disorders their sequelae.

Язык: Английский

Процитировано

0
Liangxue Qushi Zhiyang Decoction Inhibits Atopic Dermatitis in Mice via FcγR‐Mediated Phagocytosis DOI Creative Commons
Lili Zhang, Linxian Li, Zhanxue Sun

и другие.

Mediators of Inflammation, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Background: Liangxue Qushi Zhiyang Decoction (LQZ) is a traditional formula known for its efficacy in treating Atopic Dermatitis (AD). However, the specific mechanisms through which LQZ alleviates AD symptoms remain largely unknown. The objective of this study to investigate protective effects on and uncover potential action. Methods: An model was established mice using 2,4‐dinitrochlorobenzene (DNCB). Mice were then orally administered or prednisolone (PDN). Throughout treatment period, dermatitis scores scratching frequencies regularly monitored. Histopathological analyses conducted hematoxylin eosin (H&E) staining toluidine blue (TB) staining. Serum levels inflammatory cytokines measured enzyme‐linked immunosorbent assay (ELISA). Further, tandem mass tag (TMT) labeling quantitative proteomics employed identify differentially expressed proteins (DEPs). Enrichment analysis pinpoint targets pathways involved LQZ’s therapeutic Finally, validation experiments performed further explore core treatment.. Results: notably mitigated skin barrier damage response induced by DNCB mice, reduced serum IgE, IL‐4, IL‐1 β . Proteomic identified 248 with differential expression, implicating multiple LQZ’ Among these, Fc gamma R(Fc γ R)−mediated phagocytosis pathway emerged as crucial factor AD’s immune responses. Key associated pathway, including Fc‐gamma RIII (Fcgr3), V‐yes‐1 Yamaguchi sarcoma viral related oncogene homolog (Lyn), Tyrosine‐protein kinase (Syk), Phosphoinositide phospholipase C‐gamma‐2 (Plcg2), Neutrophil cytosol 1 (Ncf1), Ras‐related C3 botulinum toxin substrate 2 (Rac2) Actin‐related protein 2/3 complex subunit 3 (Arpc3), exhibited significantly expression following treatment. Conclusion: effective alleviating reactions. Its anti‐AD properties may be attributed inhibition R‐mediated phagocytic pathway.

Язык: Английский

Процитировано

0