Molecular Farming for Immunization: Current Advances and Future Prospects in Plant-Produced Vaccines
Vaccines,
Год журнала:
2025,
Номер
13(2), С. 191 - 191
Опубликована: Фев. 15, 2025
Using
plants
as
bioreactors,
molecular
farming
has
emerged
a
versatile
and
sustainable
platform
for
producing
recombinant
vaccines,
therapeutic
proteins,
industrial
enzymes,
nutraceuticals.
This
innovative
approach
leverages
the
unique
advantages
of
plants,
including
scalability,
cost-effectiveness,
reduced
risk
contamination
with
human
pathogens.
Recent
advancements
in
gene
editing,
transient
expression
systems,
nanoparticle-based
delivery
technologies
have
significantly
enhanced
efficiency
versatility
plant-based
systems.
Particularly
vaccine
development,
demonstrated
its
potential
notable
successes
such
Medicago's
Covifenz
COVID-19,
illustrating
capacity
platforms
to
address
global
health
emergencies
rapidly.
Furthermore,
edible
vaccines
opened
new
avenues
mainly
settings
low
resources
where
cold
chain
used
conventional
logistics
is
challenge.
However,
optimization
protein
yield
stability,
complexity
purification
processes,
regulatory
hurdles
are
some
challenges
that
still
remain.
review
discusses
current
status
development
using
operational
mechanisms
plant
platforms,
major
applications
prevention
infectious
diseases,
developments,
nanoparticle-mediated
cancer
vaccines.
The
discussion
will
also
touch
on
ethical
considerations,
framework,
future
trends
respect
transformative
plant-derived
ensuring
greater
accessibility
cost-effectiveness
vaccination.
field
holds
great
promise
disease
area
and,
indeed,
personalized
medicine
biopharmaceuticals
near
future.
Язык: Английский
Strategic and Technical Considerations in Manufacturing Viral Vector Vaccines for the Biomedical Advanced Research and Development Authority Threats
Vaccines,
Год журнала:
2025,
Номер
13(1), С. 73 - 73
Опубликована: Янв. 14, 2025
Over
the
past
few
decades,
world
has
seen
a
considerable
uptick
in
number
of
new
and
emerging
infectious
disease
outbreaks.
The
development
vaccines,
vaccine
technologies,
platforms
are
critical
to
enhance
our
preparedness
for
biological
threats
prevent
future
pandemics.
Viral
vectors
can
be
an
important
tool
repertoire
technologies
available
develop
effective
vaccines
against
diseases.
In
many
instances,
may
needed
reactive
scenario,
requiring
than
elicit
rapid
robust
immune
responses
with
single
dose.
Here,
we
discuss
how
viral
vector
utilized
portfolio
priority
threats,
some
challenges
manufacturing
need
strengthen
live
virus
capabilities,
opportunities
capitalize
on
use
improve
sustainability
Biomedical
Advanced
Research
Development
Authority’s
portfolio.
Язык: Английский
CRISPR-Cas: a game-changer in vaccine development and the fight against viral infections
Trends in Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 1, 2025
Язык: Английский
Application of semi-quantitative loop-mediated isothermal amplification for gene expression study in Expi293 cells.
Genes and Cells,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
BACKGROUND:
Mammalian
cell
cultures
play
a
key
role
in
the
pharmaceutical
industry,
requiring
constant
monitoring
of
conditions
during
fermentation.
In
addition
to
physical-chemical
parameters,
it
is
important
evaluate
internal
state
cells,
for
which
gene
expression
analysis
used.
Currently,
quantitative
reverse
transcription
polymerase
chain
reaction
(qPCR)
dominant
method.
However,
loop-mediated
isothermal
amplification
(LAMP)
technique
also
attracts
attention
due
its
high
specificity,
sensitivity
and
rate.
LAMP
becoming
promising
tool
rapid
expression,
especially
under
limited
biological
material
or
large
volume
samples.
AIM:
Development
quantifying
level
target
genes
human
culture
Expi293.
METHODS:
For
LAMP,
recombinant
fragment
Bacillus
stearothermophilus
DNA
(Bst-pol)
was
obtained,
purified,
optimal
were
determined.
SYBR
Green
I
LUCS13
used
as
intercalating
dyes.
The
parameters
different
concentrations
enzyme
dye
analyzed.
Standard
kits
qPCR.
Both
methods
compared
when
analyzing
IGF
1,
FGF2
EIF3i
lines
with
an
increased
these
genes.
RESULTS:
It
has
been
shown
that
using
provides
classic
S-shape
signal
accumulation
curve
while
causes
artifacts.
concentration
Bst-pol
40
ng/µl.
When
comparing
two
methods,
found
greater
sensitivity,
allows
determining
accuracy
comparable
qPCR,
demonstrating
shorter
time
(up
35
minutes).
CONCLUSION:
Although
PCR
remains
main
method
assessing
offers
number
advantages
make
attractive
alternative
various
biotechnological
purposes.
Due
speed,
ease
execution
accessibility,
well
valuable
monitoring.
Язык: Английский
Chikungunya virus release is reduced by TIM-1 receptors through binding of envelope phosphatidylserine
Journal of Virology,
Год журнала:
2024,
Номер
98(8)
Опубликована: Июль 15, 2024
ABSTRACT
T-cell
immunoglobin
and
mucin
domain
protein-1
(TIM-1)
mediates
entry
of
chikungunya
virus
(CHIKV)
into
some
mammalian
cells
through
the
interaction
with
envelope
phospholipids.
While
this
enhances
entry,
TIM-1
has
been
shown
to
tether
newly
formed
HIV
Ebola
particles,
limiting
their
efficient
release.
In
study,
we
investigate
ability
surface
receptors
such
as
sequester
budded
virions
on
infected
cells.
We
established
a
luminescence
reporter
system
produce
viral
particles
that
integrate
nano-luciferase
easily
quantify
particles.
found
host
significantly
reduced
CHIKV
release
efficiency
in
comparison
other
factors.
Removal
cell
direct
cellular
knock-out
or
altering
lipid
distribution
enhanced
Over
course
infection,
was
able
counteract
tethering
effect
by
gradually
decreasing
levels
process
mediated
nonstructural
protein
2.
This
study
highlights
importance
phosphatidylserine
mediating
not
only
but
also
its
could
aid
developing
lines
capable
vaccine
production.
IMPORTANCE
Chikungunya
is
an
enveloped
alphavirus
transmitted
bites
infectious
mosquitoes.
Infection
results
development
fever,
joint
pain,
arthralgia
can
become
chronic
last
for
months
after
infection.
Prevention
disease
still
highly
focused
vector
control
strategies.
December
2023,
new
live
attenuated
against
approved
FDA.
aimed
factors
involved
release,
better
understand
CHIKV’s
efficiently
infect
spread
among
wide
variety
lines.
abrogate
exit
information
be
beneficial
maximizing
particle
production
laboratory
settings
during
manufacturing.
Язык: Английский
Serum-Free Media Formulation Using Marine Microalgae Extracts and Growth Factor Cocktails for Madin-Darby Canine Kidney and Vero Cell Cultures
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 9881 - 9881
Опубликована: Сен. 12, 2024
The
development
of
serum-free
media
(SFM)
is
critical
to
advance
cell
culture
techniques
used
in
viral
vaccine
production
and
address
the
ethical
concerns
contamination
risks
associated
with
fetal
bovine
serum
(FBS).
This
study
evaluated
effects
marine
microalgal
extracts
growth
factor
cocktails
on
activity
Madin-Darby
canine
kidney
(MDCK)
Vero
cells.
Five
species
were
used:
Spirulina
platensis
(SP),
Dunaliella
salina
(DS),
Haematococcus
pluvialis
(HP),
Nannochloropsis
(NS),
Tetraselmis
sp.
(TS).
DS
SP
significantly
increased
proliferation
rate
both
MDCK
had
a
149.56%
195.50%
cells,
respectively,
compared
that
medium
(SFM).
Notably,
superoxide
dismutase
(SOD)
activity,
which
was
118.61%
cells
130.08%
for
DS,
108.72%
125.63%
SP,
indicating
reduction
intracellular
oxidative
stress.
Marine
extracts,
especially
are
feasible
alternatives
FBS
as
they
promote
proliferation,
ensure
safety,
supply
essential
nutrients
while
reducing
Язык: Английский
In silico studies of green fluorescent protein-tagged Hsp27-HPV16 E7 fusion protein and evaluation of cytokine secretion from antigen-presenting cells exposed to the fusion protein-carrying tumor-derived exosomes
Journal of Solid Tumors,
Год журнала:
2024,
Номер
14(1), С. 45 - 45
Опубликована: Дек. 15, 2024
Objective:
Exosome
(Exo)-based
therapies
have
attracted
considerable
interest
due
to
their
potential
as
carriers
for
therapeutic
molecules
and
capacity
elicit
anti-tumor
immune
responses.
The
objective
of
this
study
was
engineer
TC-1
tumor
cell
line-derived
exosomes
with
GFP-tagged
heat
shock
protein
(Hsp)
27-human
papillomavirus
(HPV)16
E7
fusion
evaluation
cytokine
secretion
from
antigen-presenting
cells
(APCs:
macrophages
dendritic
cells)
exposed
the
engineered
in
vitro.Methods:
In
study,
different
silico
methods
were
employed
evaluate
Hsp27-E7
Hsp27-E7-GFP
proteins
vaccine
candidates.
Regarding
data,
gene
subcloned
into
pCDH
lentiviral
vector
production
lentivirions
harboring
eukaryotic
cells.
Subsequently,
transduced
these
isolate
(i.e.,
Exo-Hsp27-E7-GFP)
using
ExoQuick-TCTM
kit
characterization
physicochemical
methods.
Finally,
key
cytokines
(IFN-γ,
TNF-α,
IL-10)
evaluated
through
incubation
(APCs)
Exo-Hsp27-E7-GFP
enzyme-linked
immunosorbent
assay
(ELISA).Results:
Our
data
showed
that
both
constructs
soluble
non-allergenic,
exhibited
strong
interaction
TLR4.
Indeed,
linkage
GFP
did
not
affect
properties,
receptors.
Moreover,
western
blot
analysis
confirmed
presence
isolated
exosomes.
could
significantly
enhance
TNF-α
IL-10
APCs
compared
Exo
Exo-GFP,
well.Conclusions:
These
vesicles
derived
demonstrate
induce
effective
immunity,
suggesting
a
promising
strategy
development
cell-free
Язык: Английский