Advances in Physalis molecular research: applications in authentication, genetic diversity, phylogenetics, functional genes, and omics DOI Creative Commons
Yan Jiang,

Yanyun Jin,

Yiyi Shan

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Июнь 27, 2024

The plants of the genus Physalis L. have been extensively utilized in traditional and indigenous Chinese medicinal practices for treating a variety ailments, including dermatitis, malaria, asthma, hepatitis, liver disorders. present review aims to achieve comprehensive up-to-date investigation , new model crop, understand plant diversity fruit development. Several chloroplast DNA-, nuclear ribosomal genomic DNA-based markers, such as psbA-trnH internal-transcribed spacer (ITS), simple sequence repeat (SSR), random amplified microsatellites (RAMS), sequence-characterized region (SCAR), single nucleotide polymorphism (SNP), were developed molecular identification, genetic diversity, phylogenetic studies species. A large number functional genes involved inflated calyx syndrome development ( AP2-L MPF2 MPF3 MAGO ), organ growth AG1 AG2 POS1 CNR1 active ingredient metabolism 24ISO DHCRT P450-CPL SR DUF538 TAS14 3β-HSB ) identified contributing breeding novel varieties. Various omic revealed functionally series reproductive development-related factors, environmental stress-responsive genes, component biosynthesis-related enzymes. chromosome-level genomes floridana Rydb., grisea (Waterf.) M. Martínez, pruinosa recently published providing valuable resource genome editing crops. Our summarizes recent progress phylogenetics, application omics accelerates efficient utilization this herb.

Язык: Английский

Role of an endodermis‐specific miR858b‐MYB1L module in the regulation of Taxol biosynthesis in Taxus mairei DOI

Chunna Yu,

D. Zhang,

Lingxiao Zhang

и другие.

The Plant Journal, Год журнала: 2025, Номер 122(1)

Опубликована: Апрель 1, 2025

SUMMARY Taxol, a chemotherapeutic agent widely used for treating various cancers, is extracted from the stems of Taxus mairei . However, current knowledge regarding effects stem tissue and age on Taxol accumulation limited. We employed matrix‐assisted laser desorption/ionization mass spectrometry to visualize taxoids in section sections varying ages T. Laser capture microdissection integrated with data‐dependent acquisition–MS/MS analysis identified that several biosynthesis pathway‐related enzymes were predominantly produced endodermis, elucidating molecular mechanisms underlying endodermis‐specific accumulation. an MYB1‐like (MYB1L) protein proposed potential function miR858‐MYB1L module regulating secondary metabolic pathways. DNA affinity purification sequencing 92 506 target peaks MYB1L. Motif enrichment de novo motifs, providing new insights into MYB recognition sites. Four MYB1L within promoter sequences synthesis genes, including TBT , DBTNBT T13OH BAPT confirmed using electrophoretic mobility shift assays. Dual‐luciferase assays showed significantly activated expression Our data indicate miR858b‐MYB1L plays crucial role transcriptional regulation by up‐regulating genes endodermis.

Язык: Английский

Процитировано

0
Metabolic and transcriptomic responses of <i>Taxus mairei</i> to nano-pollutants: insights into AgNPs and PsNPs impact DOI Creative Commons
Zhijin Wang, Pengcheng Zhang, Wei Yao

и другие.

Medicinal Plant Biology, Год журнала: 2025, Номер 4(1), С. 0 - 0

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Fungal endophytes of Taxus species and regulatory effect of two strains on taxol synthesis DOI Creative Commons
Hongshan Zhang, Wan-Ting Lin,

Ruoyun Ma

и другие.

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 3, 2024

Taxol, derived from Taxus trees, is a valuable natural resource for the development of anticancer drugs. Endophytic fungi trees are promising alternative source Taxol. However, impact plant-endophytic microbial interaction on host's Taxol biosynthesis largely unknown. In current study, diversity endophytic in three different species was analyzed using Internal Transcribed Spacer sequencing. A total 271 Operational Taxonomic Units (OTUs) were identified, grouping into 2 phyla, 8 classes, 16 orders, 19 families, and genera. Alpha beta analysis indicated significant differences fungal communities among various trees. At genus level, Alternaria Davidiella predominantly found T. mairei media, respectively. By utilizing previously published dataset, Pearson correlation conducted to predict taxol biosynthesis-related Following screening, two isolates (L7 M14) obtained. Effect inoculation with gene expression metabolite accumulation determined by transcriptomic untargeted metabolomic studies. The co-inoculation assay suggests that may have negative regulatory effect influencing hormone signaling pathways. Our findings will serve as foundation advancing production utilization also aid screening related production.

Язык: Английский

Процитировано

3
Cell type specific regulation of phenolic acid and flavonoid metabolism in Taxus mairei leaves DOI

Xiaori Zhan,

Xueshuang Liang,

Wan-Ting Lin

и другие.

Industrial Crops and Products, Год журнала: 2024, Номер 219, С. 118975 - 118975

Опубликована: Июнь 24, 2024

Язык: Английский

Процитировано

2
Advances in Physalis molecular research: applications in authentication, genetic diversity, phylogenetics, functional genes, and omics DOI Creative Commons
Yan Jiang,

Yanyun Jin,

Yiyi Shan

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Июнь 27, 2024

The plants of the genus Physalis L. have been extensively utilized in traditional and indigenous Chinese medicinal practices for treating a variety ailments, including dermatitis, malaria, asthma, hepatitis, liver disorders. present review aims to achieve comprehensive up-to-date investigation , new model crop, understand plant diversity fruit development. Several chloroplast DNA-, nuclear ribosomal genomic DNA-based markers, such as psbA-trnH internal-transcribed spacer (ITS), simple sequence repeat (SSR), random amplified microsatellites (RAMS), sequence-characterized region (SCAR), single nucleotide polymorphism (SNP), were developed molecular identification, genetic diversity, phylogenetic studies species. A large number functional genes involved inflated calyx syndrome development ( AP2-L MPF2 MPF3 MAGO ), organ growth AG1 AG2 POS1 CNR1 active ingredient metabolism 24ISO DHCRT P450-CPL SR DUF538 TAS14 3β-HSB ) identified contributing breeding novel varieties. Various omic revealed functionally series reproductive development-related factors, environmental stress-responsive genes, component biosynthesis-related enzymes. chromosome-level genomes floridana Rydb., grisea (Waterf.) M. Martínez, pruinosa recently published providing valuable resource genome editing crops. Our summarizes recent progress phylogenetics, application omics accelerates efficient utilization this herb.

Язык: Английский

Процитировано

1