Unveiling the mechanism of amelioration of adjuvant-induced rheumatoid arthritis by Drynaria quercifolia rhizome extract using network pharmacology and gene expression-based studies DOI Creative Commons
Debabrata Modak, Subhajit Ghosh, Sourav Sarkar

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 8, 2025

Rhizomes of Drynaria quercifolia have long been traditionally used to manage rheumatic pain. However, there is limited research supporting this traditional practice and insufficient evidence demonstrating the molecular mechanisms action plant-derived bioactives in rheumatoid arthritis (RA). The current study aims identify effective components methanol rhizome extract (DME) their probable pharmacological alleviating Rheumatoid Arthritis (RA) using network-pharmacology, docking, molecular-dynamics simulations, gene expression-based validation. Gas chromatography-mass spectrometry (GC-MS) based screening identified 41 volatile phytocomponents from DME having drug-like potentiality. Network pharmacology-based revealed 117 therapeutic targets for RA which 11 as core targets. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis indicated that key target genes were mostly enriched inflammatory response associated with multiple signalling pathways. Molecular docking dynamics studies proteins like serine/threonine-protein kinase (AKT1), peroxisome proliferator-activated receptor alpha (PPARA), gamma (PPARG), exhibited strong binding affinity stable interactions present DME. For experimental verification FCA (Freund's complete adjuvant)-induced chronic model employed further investigation. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) results validated significantly (p ≤ 0.05) regulate expression AKT PPARG model. Moreover, confirmed downregulated pro-inflammatory mediators COX-2, IL-6 TNF-α at protein levels also normalized different oxidative stress parameters both low high dose groups DME-treated arthritic animals. In conclusion, network-based silico approach probably act a synergistic way modulate RA, was by studies. Therefore, could be potential alternative immunomodulatory therapies combat related conditions.

Язык: Английский

Standardized Aqueous Extract of  Abutilon theophrasti Medic. Ameliorates Experimental Gastric Ulcer: A Bioinformatic and Pharmacological Study DOI
Hui Guo, Zihan Xu, Lili Zhu

и другие.

Опубликована: Янв. 1, 2025

Ethnopharmacological relevance: The herb Abutilon theophrasti Medic. (AT, Qingma in Chinese), a widely distributed medicinal plant various regions worldwide, is commonly used China for treating inflammatory diseases the gastrointestinal (GI) tract such as dysentery. However, pharmacological basis of this peptic ulcer, also an condition GI tract, remains insufficiently understood. Aim study: To investigate ameliorating effects and mechanism action standardized aqueous extract AT (ATAE) on experimental gastric ulcer (GU) rats using bioinformatic approaches.

Язык: Английский

Процитировано

0
Simultaneous Determination of 14 Bioactive Components in Fangji Huangqi Tang by UHPLC‐QqQ‐MS Technique DOI
Fangfang Xue, Lintong Xie,

Xia Zhang

и другие.

Biomedical Chromatography, Год журнала: 2025, Номер 39(5)

Опубликована: Апрель 2, 2025

Fangji Huangqi Tang (FHT) is a traditional prescription frequently utilized in clinical practice, with wide range of applications and good therapeutic effects. Quality control FHT difficult because Chinese medicine compounds usually contain vast array components characterized by significant structural diversity. A quick accurate method to determine the content active constituents was essential, which purpose quality efficacy assessment could be achieved. utilizing UHPLC-QqQ-MS technology multiple reaction monitoring (MRM) mode established quantify 14 bioactive simultaneously. These analytes included tetrandrine, fangchinoline, calycosin, calycosin-7-glucoside, medicarpin, formononetin, atractylenolide I, II, III, liquiritigenin, isoliquiritigenin, liquiritin, isoliquiritin, glycyrrhizic acid. And our knowledge, II reported for first time this paper. The thoroughly validated stable reliable application regarding specificity, linearity, precision, stability, repeatability, accuracy. allowed simultaneous determination diverse structures trace amounts FHT, ultimately achieving its safe appropriate use.

Язык: Английский

Процитировано

0
Unveiling the mechanism of amelioration of adjuvant-induced rheumatoid arthritis by Drynaria quercifolia rhizome extract using network pharmacology and gene expression-based studies DOI Creative Commons
Debabrata Modak, Subhajit Ghosh, Sourav Sarkar

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 8, 2025

Rhizomes of Drynaria quercifolia have long been traditionally used to manage rheumatic pain. However, there is limited research supporting this traditional practice and insufficient evidence demonstrating the molecular mechanisms action plant-derived bioactives in rheumatoid arthritis (RA). The current study aims identify effective components methanol rhizome extract (DME) their probable pharmacological alleviating Rheumatoid Arthritis (RA) using network-pharmacology, docking, molecular-dynamics simulations, gene expression-based validation. Gas chromatography-mass spectrometry (GC-MS) based screening identified 41 volatile phytocomponents from DME having drug-like potentiality. Network pharmacology-based revealed 117 therapeutic targets for RA which 11 as core targets. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis indicated that key target genes were mostly enriched inflammatory response associated with multiple signalling pathways. Molecular docking dynamics studies proteins like serine/threonine-protein kinase (AKT1), peroxisome proliferator-activated receptor alpha (PPARA), gamma (PPARG), exhibited strong binding affinity stable interactions present DME. For experimental verification FCA (Freund's complete adjuvant)-induced chronic model employed further investigation. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) results validated significantly (p ≤ 0.05) regulate expression AKT PPARG model. Moreover, confirmed downregulated pro-inflammatory mediators COX-2, IL-6 TNF-α at protein levels also normalized different oxidative stress parameters both low high dose groups DME-treated arthritic animals. In conclusion, network-based silico approach probably act a synergistic way modulate RA, was by studies. Therefore, could be potential alternative immunomodulatory therapies combat related conditions.

Язык: Английский

Процитировано

0