Novel Lectins from Bauhinia with Differential N-Glycan Binding Profiles DOI Creative Commons
Vinicius José Silva Osterne, Lara Dias Lima, Messias Vital Oliveira

и другие.

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

The specific interaction of lectins with carbohydrates and glycoconjugates grants these proteins a distinct ability to decode the glycocode. Essential for various biological processes in all organisms, this carbohydrate-binding activity also establishes as valuable tools fields such glycomics, medicine, biotechnology. Considering that discovery novel unique binding profiles is particularly relevant, study investigated specificity two extracted from Bauhinia seeds toward simple sugars, N-glycans O-glycans. combination agglutination-inhibition assays glycan arrays revealed subtle differences galactosides glycans containing motifs, LewisX, LacdiNAc, fucosylated LacdiNAc. Despite slight patterns, both showed similar results toxicity using Artemia salina nauplii cytotoxicity on cancer cell lines, neither lectin exhibiting significant toxicity. Additionally, demonstrated low HeLa (cervical adenocarcinoma), HT1080 (fibrosarcoma), NHDF (normal fibroblasts), even at concentrations up 125 μg/mL. Analysis partial amino acid sequences conserved residues compared other genus, well secondary structure conformations those legume lectins. This research represents advancement understanding genus Bauhinia, future structural studies could further elucidate interactions their ligands, providing fundamental insights into functions paving way potential applications.

Язык: Английский

Novel Lectins from Bauhinia with Differential N-Glycan Binding Profiles DOI Creative Commons
Vinicius José Silva Osterne, Lara Dias Lima, Messias Vital Oliveira

и другие.

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

The specific interaction of lectins with carbohydrates and glycoconjugates grants these proteins a distinct ability to decode the glycocode. Essential for various biological processes in all organisms, this carbohydrate-binding activity also establishes as valuable tools fields such glycomics, medicine, biotechnology. Considering that discovery novel unique binding profiles is particularly relevant, study investigated specificity two extracted from Bauhinia seeds toward simple sugars, N-glycans O-glycans. combination agglutination-inhibition assays glycan arrays revealed subtle differences galactosides glycans containing motifs, LewisX, LacdiNAc, fucosylated LacdiNAc. Despite slight patterns, both showed similar results toxicity using Artemia salina nauplii cytotoxicity on cancer cell lines, neither lectin exhibiting significant toxicity. Additionally, demonstrated low HeLa (cervical adenocarcinoma), HT1080 (fibrosarcoma), NHDF (normal fibroblasts), even at concentrations up 125 μg/mL. Analysis partial amino acid sequences conserved residues compared other genus, well secondary structure conformations those legume lectins. This research represents advancement understanding genus Bauhinia, future structural studies could further elucidate interactions their ligands, providing fundamental insights into functions paving way potential applications.

Язык: Английский

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