Waste Nitrogen Upcycling to Amino Acids during Anaerobic Fermentation on Biochar: An Active Strategy for Regulating Metabolic Reducing Power

Environmental Science & Technology, Год журнала: 2024, Номер 58(45), С. 20060 - 20072

Опубликована: Ноя. 1, 2024

This study proposes a novel strategy that utilizes biochar (BC) during anaerobic fermentation (AF) to generate amino acids (AAs) toward nitrogen upcycling. The BC, pyrolyzed at 800 °C (BC800) enhance graphite structures and electron-accepting sites, effectively addresses issues related biosynthetic reducing power nicotinamide adenine dinucleotide phosphate insufficiency by altering cellular conditions alleviates feedback inhibition through the immobilization of end products. process establishes unique microbial signaling energy networks, with

Язык: Английский

Investigation of geometry-dependent sensing characteristics of microfluidic for single-cell 3D localization

Review of Scientific Instruments, Год журнала: 2024, Номер 95(1)

Опубликована: Янв. 1, 2024

Flow cytometry-based measurement techniques have been widely used for single-cell characterizations, such as impedance, size, and dielectric properties. However, in the process, reliability of output signal directly affects ability microsystem to judge characteristics single cells. Here, we designed a multiple nonparallel electrode structure 3D localization. The performance structures was studied by analyzing changes electric field strength differential current. effects microchannel height, sensing distance, inclination angle, width on signals are investigated current change cells passing from center microchannel. numerical simulation results indicate that, when height is 20 µm, distance electrodes 100 angle 30°, optimal quality can be obtained. Reducing flow channel shortening spacing significantly improve amplitude. When intensity increases 80% than that 30 µm. induced with µm 42% higher 120 We analyzed presence independent adherent detection area demonstrated through caused multi-cells superimposed signals. same volume an ellipticity 1 49% lower 4. Based investigation, expect geometry design will aid development better cell impedance cytometry microsystems.

Язык: Английский

Microfluidic Sensors for the Detection of Motile Plant Zoospores

Опубликована: Янв. 1, 2024

Язык: Английский