Gradient Hydrogels Spatially Trapped Optical Cell Profiling for Quantitative Blood Cellular Osmotic Analysis DOI
Yantong Liu, Le Yu, Longfei Chen

и другие.

ACS Sensors, Год журнала: 2024, Номер 9(3), С. 1592 - 1601

Опубликована: Март 13, 2024

The quantitative exploration of cellular osmotic responses and a thorough analysis pressure-responsive behaviors are poised to offer novel clinical insights into current research. This underscores paradigm shift in the long-standing approach colorimetric measurements triggered by red cell lysis. In this study, we engineered purpose-driven optofluidic platform facilitate goal. Specifically, creating photocurable hydrogel traps surmounts persistent challenge─optical signal interference from fluid disturbances. achievement ensures stable spatial phase cells acquisition optical signals for accurate response at single-cell level. Leveraging multigradient microfluidic system, constructed gradient developed an imaging recognition algorithm, empowering comprehensive behaviors. Notably, system has successfully precisely analyzed individual clustered within dimension. Prospective testing further substantiated its feasibility performance that it demonstrates accuracy 92% discriminating complete hemolysis values (n = 25) 100% identifying initial 25). Foreseeably, strategy should promise advance pressure-related analysis, benefiting investigation diagnosis related blood diseases, quality, drug development, etc.

Язык: Английский

N2H4Zn(HC3N3O3): exceptionally strong second harmonic generation and ultra-long phosphorescence DOI Creative Commons
Can Yang,

Yuwei Kang,

Xuefei Wang

и другие.

Chemical Science, Год журнала: 2024, Номер 15(38), С. 15725 - 15730

Опубликована: Янв. 1, 2024

A novel hydro-isocyanurate, N 2 H 4 Zn(HC 3 O ), possessing an asymmetric topological diamond-like 3D network exhibits the strongest second harmonic generation among cyanurates (13 × KDP) and ultra-long room-temperature phosphorescence (448 ms).

Язык: Английский

Процитировано

3

Rapid and sensitive on-site detection of Clothianidin in surface waters with a reusable fiber-embedded optofluidic biochip DOI

Laiya Lu,

Min Wang,

Tianxiang Ji

и другие.

Опубликована: Апрель 21, 2025

Abstract Clothianidin (CLO), a highly effective neonicotinoid insecticide, is globally utilized to combat both sucking and chewing pests. There an increasing demand for rapid high-frequency on-site detection of CLO in water food sources due its high toxicity non-target organisms. To address this, we introduce innovative decentralized testing method employing reusable fiber-embedded optofluidic biochip. This biochip leverages evanescent wave fluorescence, indirect competitive immunoassay, technology provide reliable, rapid, straightforward, cost-effective measurements. The integration all-fiber optical structure with tapered fiber nano-biosensor significantly enhances fluorescence excitation collection efficiency, bolstering the biochip's capabilities scalability. demonstrated sensitivity specificity detecting CLO, achieving satisfactory limit 1.0 µg/L within 12 min. It was successfully applied screening surface waters Beijing-Tianjin region, offering timely feedback. detected spiked recovery rates. These confirm potential as robust tool food, particularly settings limited resources. adaptable can be easily expanded detect other trace pollutants by utilizing appropriate functionalized biosensors antibodies.

Язык: Английский

Процитировано

0

Design and Characterization of Bionic Lymphatic Valve Based on Thin Film Shell Theory DOI

Lixia Yang,

Shaohua Zuo,

Wenhao Yin

и другие.

Journal of Bionic Engineering, Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Язык: Английский

Процитировано

0

Rapid and sensitive on-site detection of clothianidin in surface waters with a reusable fiber-embedded optofluidic biochip DOI

Laiya Lu,

Min Wang,

Tianxiang Ji

и другие.

Microchimica Acta, Год журнала: 2025, Номер 192(6)

Опубликована: Май 23, 2025

Язык: Английский

Процитировано

0

A microfluidic hemostatic diagnostics platform: Harnessing coagulation-induced adaptive-bubble behavioral perception DOI Creative Commons
Longfei Chen, Le Yu, Ming Chen

и другие.

Cell Reports Medicine, Год журнала: 2023, Номер 4(11), С. 101252 - 101252

Опубликована: Окт. 24, 2023

Clinical viscoelastic hemostatic assays, which have been used for decades, rely on measuring biomechanical responses to physical stimuli but face challenges related high device and test cost, limited portability, scalability.. Here, we report a differential pattern using self-induced adaptive-bubble behavioral perception refresh it. The adaptive behaviors of bubble deformation during coagulation precisely describe the transformation properties, being free precise complex devices. And integrated array chip allows microassays enables multi-bubble tests with good reproducibility. Recognition developed empowers automated user-friendly diagnosis. In prospective clinical study (clinical model development [n = 273]; assay 44]), show that diagnostic accuracies were 99.1% key indicators (reaction time [R], kinetics [K], alpha angle [Angle], maximum amplitude [MA], lysis at 30 min [LY30]; n 220) 100% (n 44) hypercoagulation, healthy, hypocoagulation diagnoses. This should provide fresh insight into existing paradigms help more needs.

Язык: Английский

Процитировано

7

Gradient Hydrogels Spatially Trapped Optical Cell Profiling for Quantitative Blood Cellular Osmotic Analysis DOI
Yantong Liu, Le Yu, Longfei Chen

и другие.

ACS Sensors, Год журнала: 2024, Номер 9(3), С. 1592 - 1601

Опубликована: Март 13, 2024

The quantitative exploration of cellular osmotic responses and a thorough analysis pressure-responsive behaviors are poised to offer novel clinical insights into current research. This underscores paradigm shift in the long-standing approach colorimetric measurements triggered by red cell lysis. In this study, we engineered purpose-driven optofluidic platform facilitate goal. Specifically, creating photocurable hydrogel traps surmounts persistent challenge─optical signal interference from fluid disturbances. achievement ensures stable spatial phase cells acquisition optical signals for accurate response at single-cell level. Leveraging multigradient microfluidic system, constructed gradient developed an imaging recognition algorithm, empowering comprehensive behaviors. Notably, system has successfully precisely analyzed individual clustered within dimension. Prospective testing further substantiated its feasibility performance that it demonstrates accuracy 92% discriminating complete hemolysis values (n = 25) 100% identifying initial 25). Foreseeably, strategy should promise advance pressure-related analysis, benefiting investigation diagnosis related blood diseases, quality, drug development, etc.

Язык: Английский

Процитировано

2