RNA aptamer-based CRISPR-Cas12a system for enhanced small molecule detection and point-of-care testing

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 303, С. 140675 - 140675

Опубликована: Фев. 4, 2025

Язык: Английский

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Application of CRISPR/Cas12a in miRNA-155 detection: A novel homogeneous electrochemiluminescence biosensor

Analytica Chimica Acta, Год журнала: 2024, Номер 1316, С. 342843 - 342843

Опубликована: Июнь 7, 2024

Язык: Английский

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Binding-Induced Activation of CRISPR-Cas12a Using PAM-Engineered Toehold Switch and DNA Tile Architectures for Enhanced Detection of Thrombin

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 421, С. 136547 - 136547

Опубликована: Авг. 29, 2024

Язык: Английский

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Electrochemical Detection of MMP-2 with Enhanced Sensitivity: Utilizing T7 RNA Polymerase and CRISPR-Cas12a for Neuronal Studies

Talanta, Год журнала: 2024, Номер 281, С. 126795 - 126795

Опубликована: Сен. 1, 2024

Язык: Английский

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Hybridization chain reaction and CRISPR/Cas12a-integrated biosensor for precise Ago2 detection

Bioelectrochemistry, Год журнала: 2025, Номер unknown, С. 108975 - 108975

Опубликована: Март 1, 2025

Язык: Английский

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Allosteric Ribozyme-Driven crRNA Switch for the Amplification-Free Detection of Biomolecules

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117450 - 117450

Опубликована: Апрель 1, 2025

Язык: Английский

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Integrating PfAgo cleavage with DNA walker amplification for Attomolar-Level detection of HPV16 DNA

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 114016 - 114016

Опубликована: Май 1, 2025

Язык: Английский

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CRISPR/Cas12a-Based Biosensing: Advances in Mechanisms and Applications for Nucleic Acid Detection

Biosensors, Год журнала: 2025, Номер 15(6), С. 360 - 360

Опубликована: Июнь 4, 2025

Nucleic acid detection technology is crucial for molecular diagnosis. The advent of CRISPR/Cas12a-based nucleic has considerably broadened its scope, from the identification infectious disease-causing microorganisms to disease-associated biomarkers. This innovative system capitalizes on non-specific single-strand cleavage activity Cas12a upon target DNA recognition. By employing a fluorescent probe in form single-stranded DNA/RNA, this enables observation fluorescence changes resulting nonspecific cleavage, thereby facilitating detection. systems can be regarded as new type biosensor, offering practical and efficient approach analysis various diagnostic settings. biosensors outperform conventional methods terms portability, simplicity, speed, efficiency. In review, we elucidate principle their application disease diagnostics discuss recent innovations technological challenges, aiming provide insights research further development personalized medicine. Our findings show that although have considerable potential applications theoretical research, certain challenges remain. These include simplifying reaction process, enhancing precision, broadening scope detection, translation into clinical practice. We anticipate ongoing advancements will address these challenges.

Язык: Английский

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A portable microfluidic chip-based fluorescent and colorimetric aptasensor combining recombinase polymerase amplification for bovine pregnancy-associated glycoproteins detection

Biosensors and Bioelectronics, Год журнала: 2024, Номер 270, С. 116981 - 116981

Опубликована: Ноя. 21, 2024

Язык: Английский

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Split CRISPR-Cas12a for enhanced detection of NF-κB p50: A novel electrochemiluminescence biosensor approach

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 111621 - 111621

Опубликована: Сен. 1, 2024

Язык: Английский

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