Single Particle Analysis-Enhanced DNA Walking Machine for Sensitive miRNA Detection

Analytical Chemistry, Год журнала: 2024, Номер 96(28), С. 11566 - 11571

Опубликована: Июнь 28, 2024

DNA walking machines have achieved significant breakthroughs in areas such as biosensing, bioimaging, and early cancer diagnosis, facilitated by the self-assembly of or its combination with other materials, magnetic beads metal nanoparticles. However, current machine strategies are constantly challenged inadequate analytical sensitivity, while sophisticated signal amplification procedures often indispensable. Single-particle inductively coupled plasma mass spectrometry (SP-ICPMS) provides superior sensitivity can effectively discriminate between background noise detected signals due to large number atoms a nanoparticle concentrating effect single detection. In this study, we present novel approach utilizing counting duplex-specific nuclease (DSN)-assisted construct 3D for detecting aggressive prostate (PCa) biomarker miRNA-200c. The proposed strategy showed an improvement detection limit (LOD) 0.93 pM (28 amol) was successfully applied human serum samples. To best our knowledge, is first report study.

Язык: Английский

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The Evolution of Nucleic Acid Nanotechnology: From DNA Assembly to DNA‐Encoded Library

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Abstract Deoxyribonucleic acid (DNA), a fundamental biomacromolecule in living organisms, serves as the carrier of genetic information. Beyond its role encoding biological functions, DNA's inherent ability to hybridize through base pairing has opened new avenues for application sciences. This review introduces DNA nanotechnology and DNA‐encoded library (DEL), highlights their shared design principles related assembly. First, foundational overview structural nanotechnology, including strategies historical development is provided. Subsequently, various approaches are examined dynamic from strand displacement reactions DNA‐templated polymer synthesis. Second, how principle assembly facilitated diverse formats self‐assembly‐based DEL synthesis, DNA‐template (DTS), template‐mediated proximity induction effects examined. These advancements all underpinned by unique property Finally, this summarizes common terms methodology design. Additionally, potential synergies explored between these two technologies, envisioning future applications where they can be combined create more versatile exquisite functionalities.

Язык: Английский

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Fluorescence detection of the genetic risk factor ApoE4 gene associate with Alzheimer’s disease based on a CRISPR-Cas12a system

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112831 - 112831

Опубликована: Янв. 1, 2025

Язык: Английский

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An Ultrasensitive and Specific Fluorescence Split-Aptasensor for D-Vp Detection Based on Target-Induced Self-Propelled 3d-Dna Walkers Coupled with Crispr-Cas12a

Опубликована: Янв. 1, 2025

Язык: Английский

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Perspectives of Exosomal ncRNAs in the Treatment of Bone Metabolic Diseases: Focusing on Osteoporosis, Osteoarthritis, and Rheumatoid Arthritis

Experimental Cell Research, Год журнала: 2025, Номер unknown, С. 114457 - 114457

Опубликована: Фев. 1, 2025

Язык: Английский

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A Patient-Centered Approach in Sensor Science: Embracing Patient Engagement for Translational Clinical Technologies

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

With the goal of impacting patient quality life and outcomes, sensor science offers significant potential to revolutionize healthcare by providing advances in detection molecular biomarkers for personalized clinical technologies. The community has achieved technical advancements that can impact diagnostics, health monitoring, disease treatment; however, many innovations remain confined laboratory, failing bridge translational gap between research real-world applications. This perspective presents a new direction community, where development centers on needs experiences primary beneficiaries: patients. We provide guidelines resources researchers engage with patients early continuously throughout process inform specifications better align technologies needs, improving their adoption impact. also present examples implementing patient-centered approach planning engagement research. In design impactful sensors patients, must expand focus beyond embrace approach, which will likely lead opportunities collaboration evolution community.

Язык: Английский

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An ultrasensitive and specific fluorescence split-aptasensor for D-VP detection based on target-induced self-propelled 3D DNA walkers coupled with CRISPR-Cas12a

Talanta, Год журнала: 2025, Номер 293, С. 128102 - 128102

Опубликована: Апрель 8, 2025

Язык: Английский

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CRISPR: The frontier technology of next-generation RNA detection

Biochemical Engineering Journal, Год журнала: 2024, Номер 211, С. 109480 - 109480

Опубликована: Авг. 28, 2024

Язык: Английский

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Magnetic nanobead assisted the dual targets driven fluorescent biosensor based on SPEXPAR and MNAzyme for the Olfactory Marker Protein detection

Materials Today Bio, Год журнала: 2024, Номер 29, С. 101272 - 101272

Опубликована: Сен. 27, 2024

Язык: Английский

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Toehold and Hairpin Assembly-Mediated Tripedal DNA Walker for Circulating Tumor DNA Detection in Human Blood

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 424, С. 136887 - 136887

Опубликована: Ноя. 1, 2024

Язык: Английский

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