Recent Advances in Polymer Science and Fabrication Processes for Enhanced Microfluidic Applications: An Overview

Micromachines, Год журнала: 2024, Номер 15(9), С. 1137 - 1137

Опубликована: Сен. 6, 2024

This review explores significant advancements in polymer science and fabrication processes that have enhanced the performance broadened application scope of microfluidic devices. Microfluidics, essential biotechnology, medicine, chemical engineering, relies on precise fluid manipulation micrometer-sized channels. Recent innovations materials, such as flexible, biocompatible, structurally robust polymers, been pivotal developing advanced systems. Techniques like replica molding, microcontact printing, solvent-assisted injection 3D printing are examined, highlighting their advantages recent developments. Additionally, discusses diverse applications polymer-based devices biomedical diagnostics, drug delivery, organ-on-chip models, environmental monitoring, industrial processes. paper also addresses future challenges, including enhancing resistance, achieving multifunctionality, ensuring biocompatibility, scaling up production. By overcoming these potential for widespread adoption impactful use technologies can be realized.

Язык: Английский

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‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels

Nanomaterials, Год журнала: 2025, Номер 15(2), С. 104 - 104

Опубликована: Янв. 10, 2025

Microfluidic sensors incorporated onto chips allow sensor miniaturization and high-throughput analyses for point-of-care or non-clinical analytical tools. Three-dimensional (3D) printing based on femtosecond laser direct writing (fs-LDW) is useful creating 3D microstructures with high spatial resolution because the structures are printed in space along a designated light path. High-performance biochips can be fabricated using ‘ship-in-a-bottle’ integration technique, which functional microcomponents biomimetic embedded inside closed microchannels fs-LDW. Solutions containing protein biomacromolecules as precursor used to fabricate that retain their native functions. Here, we demonstrate ship-in-a-bottle of pure proteinaceous exhibit pH sensitivity. We mesh gap sizes 10 5 μm. The these gaps changed when exposed physiological buffers ranging from 4 10. size shrunk expanded repeatedly by changing surrounding buffer. Fs-LDW enables construction microscopic meshes dynamic functions such sensing might find applications filtering particles microfluidic channels.

Язык: Английский

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Fluorescent Molecular Probe for Imaging Hypoxia in 2D Cell Culture Monolayers and 3D Tumor Spheroids: The Cell Membrane Partition Model for Predicting Probe Distribution in a Spheroid

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

Compared to cultured 2D cell monolayers, 3D multicellular spheroids are more realistic tumor models. Nonetheless, remain under-utilized in preclinical research, part, because there is a lack of fluorescence sensors that can noninvasively interrogate all the individual cells within spheroid. This present study describes deep-red fluorogenic molecular probe for microscopic imaging contain high level nitroreductase enzyme activity as biomarker hypoxia. A first-generation version produced "turn-on" monolayer under hypoxic conditions; however, it was not useful spheroid only accumulated peripheral cells. To guide structural optimization process, an intuitive theoretical membrane partition model conceived predict how dosed will distribute The identifies three limiting diffusion pathways determined by probe's properties. lipophilic with affinity rapidly becomes trapped membranes In contrast, very hydrophilic molecule negligible diffuses through intercellular space and rarely enters However, intermediate undergoes sequential out distributes Using predictive tool, second-generation fluorescent prepared smaller structure, optical sectioning using structured illumination or light sheet microscopy revealed roughly even throughout permeation likely be broadly applicable various classes molecules nanoparticles enable distribution

Язык: Английский

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オルガノイド (organoid)

The Japanese Journal of SURGICAL METABOLISM and NUTRITION, Год журнала: 2025, Номер 59(1), С. 30 - 31

Опубликована: Фев. 15, 2025

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Advances in humanoid organoid-based research on inter-organ communications during cardiac organogenesis and cardiovascular diseases

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Март 28, 2025

The intimate correlation between cardiovascular diseases and other organ pathologies, such as metabolic kidney diseases, underscores the intricate interactions among these organs. Understanding inter-organ communications is crucial for developing more precise drugs effective treatments systemic diseases. While animal models have traditionally been pivotal in studying interactions, human-induced pluripotent stem cells (hiPSCs) offer distinct advantages when constructing vitro models. Beyond conventional two-dimensional co-culture model, hiPSC-derived humanoid organoids emerged a substantial advancement, capable of replicating essential structural functional attributes internal organs vitro. This breakthrough has spurred development multilineage organoids, assembloids, organoids-on-a-chip technologies, which allow enhanced physiological relevance. These technologies shown great potential mimicking coordinated organogenesis, exploring disease pathogenesis, facilitating drug discovery. As central system, heart serves focal point an extensively studied network interactions. review focuses on advancements challenges organs, presenting comprehensive exploration this cutting-edge approach research.

Язык: Английский

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Advancements of paper-based microfluidics and organ-on-a-chip models in cosmetics hazards

RSC Advances, Год журнала: 2025, Номер 15(13), С. 10319 - 10335

Опубликована: Янв. 1, 2025

Different detection approaches for monitoring adulterants/hazards present in cosmetics using paper-based devices and organ-on-a-chip.

Язык: Английский

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Artificial intelligence in preclinical research: enhancing digital twins and organ-on-chip to reduce animal testing

Drug Discovery Today, Год журнала: 2025, Номер unknown, С. 104360 - 104360

Опубликована: Апрель 1, 2025

Artificial intelligence (AI) is reshaping preclinical drug research offering innovative alternatives to traditional animal testing. Advanced techniques, including machine learning (ML), deep (DL), AI-powered digital twins (DTs), and AI-enhanced organ-on-a-chip (OoC) platforms, enable precise simulations of complex biological systems. AI plays a critical role in overcoming the limitations DTs OoC, improving their predictive power scalability. These technologies facilitate early-stage, reliable evaluations safety efficacy, addressing ethical concerns, reducing costs, accelerating development while adhering 3Rs principle (Replace, Reduce, Refine). By integrating with these advanced models, can achieve greater accuracy efficiency discovery. This review examines transformative impact research, highlighting its advancements, challenges, steps needed establish as cornerstone efficient

Язык: Английский

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Drug Screening of Primary Human Endometriotic Cells Based on Micro‐Encapsulating Microfluidic Chip

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Abstract Endometriosis (EMs), a significant global health issue, characterized by unclear pathogenesis, nonspecific symptoms, and poor treatment outcomes. The organ‐on‐chip technology has achieved great advances in disease modeling, yet its potential EMs‐related research remains largely untapped. Herein, microfluidic chip platform that integrates primary cell‐laden microcapsules for personalized drug evaluation. Specifically, human ectopic endometrial stromal cells (hESCs) within featuring biocompatible carboxymethyl cellulose (CMC) core stable alginate (ALG) shell using precise electrospray are encapsulated. These integrated into with branched gradient generator multiple cell‐culture chambers, enabling tailored high‐throughput screening. By exposing hESCs‐microcapsules derived from of distinct patient individuals to various drugs on‐chip, inter‐individual variability was revealed, strong correlation clinical This unique combination patient‐specific 3D microenvironments dynamic control represents paradigm shift EMs research. Further integrating omics techniques, capability exploring promising is showcased. results reveal the could deliver dependable screening outcomes, thereby benefiting both scientific inquiries therapies.

Язык: Английский

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Advancing Organ-on-a-Chip Systems: The Role of Scaffold Materials and Coatings in Engineering Cell Microenvironment

Polymers, Год журнала: 2025, Номер 17(9), С. 1263 - 1263

Опубликована: Май 6, 2025

For organ-on-a-chip (OoC) engineering, the use of biocompatible coatings and materials is not only recommended but essential. Extracellular matrix (ECM) components are commonly used as due to their effects on cell orientation, protein expression, differentiation, adhesion. Among most frequently collagen, fibronectin, Matrigel, according specific type intended OoC application. Additionally, such polydimethylsiloxane (PDMS), thermoplastics, chitosan, alginate serve scaffolding biomechanical properties biocompatibility. Here, we discuss some employed coating techniques, including SAMs, dip coating, spin microcontact printing, 3D bioprinting, each offering advantages drawbacks. Current challenges comprise enhancing biocompatibility, exploring novel materials, improving scalability reproducibility.

Язык: Английский

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Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108595 - 108595

Опубликована: Май 1, 2025

Язык: Английский

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