Adding a Twist to Lateral Flow Immunoassays: A Direct Replacement of Antibodies with Helical Affibodies, from Selection to Application DOI Creative Commons

Chris Sadler,

Adam Creamer, Kim Anh Giang

и другие.

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Immunoreagents, most commonly antibodies, are integral components of lateral flow immunoassays. However, the use antibodies comes with limitations, particularly relating to their reproducible production, and poor thermal chemical stability. Here, we employ phage display develop affibodies, a class nonimmunoglobulin affinity proteins based on small three-helix bundle scaffold, against SARS-CoV-2 Spike protein. Subsequently, demonstrate utility viability affibodies directly replace in In addition, highlight several physiochemical advantages including ability withstand exposure high temperature humidity while maintaining superior performance compared antibody counterparts. Furthermore, investigate adsorption mechanism surface gold nanoparticle probes via His6-tag, introduced also facilitate recombinant purification. Through molecular dynamics simulations, elucidate structural physical characteristics affibody dimers which result high-performing detection when immobilized surfaces. This work demonstrates that can be used as direct replacements immunoassays should further considered alternatives owing improved properties modular design.

Язык: Английский

A Patient-Centered Approach in Sensor Science: Embracing Patient Engagement for Translational Clinical Technologies DOI

Alana F. Ogata,

Julia Kramer

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

With the goal of impacting patient quality life and outcomes, sensor science offers significant potential to revolutionize healthcare by providing advances in detection molecular biomarkers for personalized clinical technologies. The community has achieved technical advancements that can impact diagnostics, health monitoring, disease treatment; however, many innovations remain confined laboratory, failing bridge translational gap between research real-world applications. This perspective presents a new direction community, where development centers on needs experiences primary beneficiaries: patients. We provide guidelines resources researchers engage with patients early continuously throughout process inform specifications better align technologies needs, improving their adoption impact. also present examples implementing patient-centered approach planning engagement research. In design impactful sensors patients, must expand focus beyond embrace approach, which will likely lead opportunities collaboration evolution community.

Язык: Английский

Процитировано

0

Adding a Twist to Lateral Flow Immunoassays: A Direct Replacement of Antibodies with Helical Affibodies, from Selection to Application DOI Creative Commons

Chris Sadler,

Adam Creamer, Kim Anh Giang

и другие.

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Immunoreagents, most commonly antibodies, are integral components of lateral flow immunoassays. However, the use antibodies comes with limitations, particularly relating to their reproducible production, and poor thermal chemical stability. Here, we employ phage display develop affibodies, a class nonimmunoglobulin affinity proteins based on small three-helix bundle scaffold, against SARS-CoV-2 Spike protein. Subsequently, demonstrate utility viability affibodies directly replace in In addition, highlight several physiochemical advantages including ability withstand exposure high temperature humidity while maintaining superior performance compared antibody counterparts. Furthermore, investigate adsorption mechanism surface gold nanoparticle probes via His6-tag, introduced also facilitate recombinant purification. Through molecular dynamics simulations, elucidate structural physical characteristics affibody dimers which result high-performing detection when immobilized surfaces. This work demonstrates that can be used as direct replacements immunoassays should further considered alternatives owing improved properties modular design.

Язык: Английский

Процитировано

0