Recent advances in single fluorescent probes for monitoring dual organelles in two channels DOI Creative Commons

Xiu‐Zhi Yang,

Shankun Yao,

Jisong Wu

и другие.

Smart Molecules, Год журнала: 2024, Номер unknown

Опубликована: Дек. 15, 2024

Abstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of organelle networks associated multiple pathological processes. However, clearly intuitively visualizing highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due distinct advantages facile, non‐invasiveness detection, making it particularly well‐suited applications in uncovering mysterious veil interactions. Regrettably, lack ideal fluorescence agents has always been an obstacle intricate behaviors organelles. In this review, we provide a systematic discussion on existing dual‐color dual‐targetable molecular sensors used monitoring interactions, focus their targeting strategies, mechanisms biological applications. Additionally, current limitations future development directions probes dual‐emissives briefly discussed. This review aims guidance researchers develop more improved studying biomedical field.

Язык: Английский

Ether Rhodamines with Enhanced Hydrophilicity, Fluorogenicity, and Brightness for Super-Resolution Imaging DOI Open Access

Xiangning Fang,

Qinglong Qiao,

Z W Li

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

ABSTRACT Rhodamine dyes are widely used fluorophores in super-resolution fluorescence imaging due to their exceptional optical properties and “aggregation-disaggregation” induced fluorogenic activation. However, excessive lipophilicity often reduces brightness aqueous environments causes off-target staining, limiting effectiveness high-resolution imaging. To address these challenges, we introduce an ether-decorated N-terminal modification strategy for rhodamine silicon-rhodamine (Si-rhodamine), replacing conventional N-alkyl groups. The ether chains enhance water solubility, decrease aggregate size, improve fluorogenicity across a wide concentration range. Their flexible, hydrophilic structure forms protective shield around the xanthene core, minimizing dye-water interactions reducing quenching. Additionally, inductive effect of decreases electron-donating strength amino groups, suppressing quenching caused by twisted intramolecular charge transfer (TICT). These modifications collectively increase quantum yields ER ESiR from 0.35 0.19 (for tetraethyl-substituted analogs) 0.70 0.41, respectively. Probes derived exhibit outstanding fluorogenicity, enhanced signal-to-noise ratios, improved resolution complex environments, demonstrating superior performance advanced techniques such as structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy, single-molecule localization (SMLM). This work introduces innovative fluorophore design, offering significant advancements applications.

Язык: Английский

Процитировано

0
Designing malachite green derivatives to optimize fluorogen-activating protein pairs for rapid PAINT super-resolution imaging in living cells DOI

Xiangning Fang,

Qinglong Qiao, Fēi Dèng

и другие.

Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 110980 - 110980

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Dibutylated Si-fluorescein: enhanced hydrophobicity for fluorogenic labeling in vivo. DOI
Ning Wan, Haoyang Li, David C. M. Kong

и другие.

Dyes and Pigments, Год журнала: 2025, Номер unknown, С. 112841 - 112841

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Azetidinyl Malachite Green: a Superior Fluorogen-Activating Protein Probe for Live-cell and Dynamic SIM Imaging DOI Creative Commons
Fei Deng,

Xiangning Fang,

Qinglong Qiao

и другие.

Chemical Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Azetidinyl Malachite Green (Aze-MG) enhances the brightness and photostability of MG-FAP system by suppressing TICT effect, enabling stable dynamic super-resolution imaging through a buffering strategy.

Язык: Английский

Процитировано

0
Super-resolution imaging of cellular pseudopodia dynamics with a target-specific blinkogenic probe DOI
Aimin Song, Qinglong Qiao, Ning Xu

и другие.

Chinese Chemical Letters, Год журнала: 2024, Номер unknown, С. 110643 - 110643

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

3
Recent advances in single fluorescent probes for monitoring dual organelles in two channels DOI Creative Commons

Xiu‐Zhi Yang,

Shankun Yao,

Jisong Wu

и другие.

Smart Molecules, Год журнала: 2024, Номер unknown

Опубликована: Дек. 15, 2024

Abstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of organelle networks associated multiple pathological processes. However, clearly intuitively visualizing highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due distinct advantages facile, non‐invasiveness detection, making it particularly well‐suited applications in uncovering mysterious veil interactions. Regrettably, lack ideal fluorescence agents has always been an obstacle intricate behaviors organelles. In this review, we provide a systematic discussion on existing dual‐color dual‐targetable molecular sensors used monitoring interactions, focus their targeting strategies, mechanisms biological applications. Additionally, current limitations future development directions probes dual‐emissives briefly discussed. This review aims guidance researchers develop more improved studying biomedical field.

Язык: Английский

Процитировано

2