Application of CRISPR-Cas System in HPV Detection Using Biosensor Devices and POC Technologies

BME Frontiers, Год журнала: 2025, Номер 6

Опубликована: Янв. 1, 2025

Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.

Язык: Английский

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A Patient-Centered Approach in Sensor Science: Embracing Patient Engagement for Translational Clinical Technologies

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

With the goal of impacting patient quality life and outcomes, sensor science offers significant potential to revolutionize healthcare by providing advances in detection molecular biomarkers for personalized clinical technologies. The community has achieved technical advancements that can impact diagnostics, health monitoring, disease treatment; however, many innovations remain confined laboratory, failing bridge translational gap between research real-world applications. This perspective presents a new direction community, where development centers on needs experiences primary beneficiaries: patients. We provide guidelines resources researchers engage with patients early continuously throughout process inform specifications better align technologies needs, improving their adoption impact. also present examples implementing patient-centered approach planning engagement research. In design impactful sensors patients, must expand focus beyond embrace approach, which will likely lead opportunities collaboration evolution community.

Язык: Английский

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CRISPR/Cas System-Based Fluorescent Sensor for Analysis and Detection

Critical Reviews in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 1 - 16

Опубликована: Март 24, 2025

Fluorescent sensor is an important tool to reliaze qualitative or quantitative detection of target analyte based on the fluorescence principle. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) has been utilized develop as a precise, efficient, and highly sensitive molecular diagnostic due its efficient targeting gene editing ability. At present, CRISPR/Cas system-based fluorescent sensors have shown excellent performance in field analysis detection, received widespread attention. Therefore, this paper reviews mechanism system, characteristics different Cas proteins, principle sensor, with focus summarizing application for detection.

Язык: Английский

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Machine learning and statistical classification in CRISPR-Cas12a diagnostic assays

Biosensors and Bioelectronics, Год журнала: 2025, Номер 279, С. 117402 - 117402

Опубликована: Март 31, 2025

CRISPR-based diagnostics have gained increasing attention as biosensing tools able to address limitations in contemporary molecular diagnostic tests. To maximize the performance of assays, much effort has focused on optimizing chemistry and biology reaction. However, less been paid improving techniques used analyze data. date, decisions typically involve various forms slope-based classification. Such methods are superior traditional based assessing absolute signals, but still limitations. Herein, we establish benchmarks (total accuracy, sensitivity, specificity) using common methods. We compare these benchmark with three different quadratic empirical distribution function statistical tests, finding significant improvements speed accuracy when applied a clinical data set. Two techniques, Kolmogorov-Smirnov Anderson-Darling report lowest time-to-result highest total test accuracy. Furthermore, developed long short-term memory recurrent neural network classify CRISPR-biosensing data, achieving 100 % specificity our model Finally, provide guidelines choosing classification method parameters that best suit assay's needs.

Язык: Английский

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Enzyme-Free Amplification-Enhanced Ratiometric Fluorescence Method for Highly Sensitive Detection of Acute Myocardial Infarction Biomarkers

Biosensors and Bioelectronics, Год журнала: 2025, Номер 283, С. 117527 - 117527

Опубликована: Апрель 28, 2025

Язык: Английский

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CRISPR/Cas12a-based fluorescence-colorimetric dual-mode strategy for sensitive detection of Clear cell renal cell carcinoma biomarker miR-210-3p

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137923 - 137923

Опубликована: Май 1, 2025

Язык: Английский

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