CRISPR Digital Sensing: From Micronano-Collaborative Chip to Biomolecular Detection DOI
Xinyi Wang, Longjie Zhong, Wenbin Zhang

и другие.

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Май 24, 2025

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) sensing technology proved to be valuable during the COVID-19 pandemic through its sensitivity, specificity, robustness, and versatility. However, issues such as overreliance on amplification, susceptibility false positives, lack of quantification strategies, complex operation procedures have hindered broader application in bioanalysis clinical diagnostics. collision between micronano-collaborative chips CRISPR has effectively addressed these bottlenecks, offering innovative solutions for diagnosis treatment. Unlike conventional micronano chips, digital enhance CRISPR's response trace amounts target molecules by leveraging highly controllable local environments compartmentalized microreactors. This advancement improves detection efficiency revolutionizes traditional vitro bioanalytical processes. First, working principles, fabrication techniques, performance metrics CRISPR-based droplet microfluidics microarray are examined. Then, applications bioassays reviewed, emphasizing their importance advancing systems gene editing. Finally, prospects explored, particularly potential body surface biomonitoring development opportunities biomedical field.

Язык: Английский

Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges DOI Creative Commons

Heyjin Son

Biosensors, Год журнала: 2024, Номер 14(10), С. 460 - 460

Опубликована: Сен. 26, 2024

The emergence of CRISPR/Cas systems has revolutionized the field molecular diagnostics with their high specificity and sensitivity. This review provides a comprehensive overview principles recent advancements in harnessing for detecting DNA RNA. Beginning an exploration mechanisms key Cas proteins underpinning systems, navigates detection both pathogenic non-pathogenic nucleic acids, emphasizing pivotal role CRISPR identifying diverse genetic materials. discussion extends to integration various signal-readout techniques, including fluorescence, electrochemical, colorimetric, as well imaging biosensing methods, highlighting advantages limitations practical applications. Furthermore, critical analysis challenges field, such target amplification, multiplexing, quantitative detection, underscores areas requiring further refinement. Finally, concludes insights into future directions CRISPR-based acid potential these continue driving innovation diagnostics, broad implications research, clinical practice, biotechnology.

Язык: Английский

Процитировано

6
CRISPR/Cas-powered “sweet” DNA nano-sponge for highly sensitive detection of pathogen nucleic acid with portable personal glucometer DOI

Huiling You,

Jingwen Shan,

Yang Bai

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160618 - 160618

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Deactivated Cas9-Engineered Magnetic Micromotors toward a Point-of-Care Digital Viral RNA Assay DOI
Younseong Song, Hyunji Park, Prudhvi Thirumalaraju

и другие.

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Digital nucleic acid assays, known for their high sensitivity and specificity, typically rely on fluorescent readouts expensive complex nanowell manufacturing, which constrain broader use in point-of-care (POC) application. Here, we introduce an alternative digital molecular diagnostics, termed dCRISTOR, by seamlessly integrating deactivated Cas9 (dCas9)-engineered micromotors, extraction-free loop-mediated isothermal amplification (LAMP), low-cost bright field microscopy, deep learning-enabled image processing. The micromotor, composed of a polystyrene sphere attached to magnetic bead, incorporates dCas9 ribonucleoprotein complex. presence human immunodeficiency virus-1 (HIV-1) RNA sample results the formation large-sized amplicons that can be specifically captured reducing velocity induced external field. micromotor is propelled field, eliminates need chemical fuels, system complexity, allowing precise control over movement, enhancing accuracy reliability. A convolutional neural network classification-based multiobject tracking algorithm, CNN-MOT, accurately measures change motion, facilitating binary assay format ("1" or "0") simplified result interpretation without user bias. Incorporating LAMP streamlines dCRISTOR workflow, enabling qualitative HIV-1 detection spiked plasma (n = 21) demonstrates 100% specificity achieves limit (LOD) 0.96 copies/μL. also achieved correlation with reverse transcription-quantitative polymerase chain reaction (RT-qPCR) clinical patient samples 9). assay, label-free testing fluorescence readouts, absorbance measurements, manufacturing processes, represents substantial advancement viral diagnostics.

Язык: Английский

Процитировано

0
A Patient-Centered Approach in Sensor Science: Embracing Patient Engagement for Translational Clinical Technologies DOI

Alana F. Ogata,

Julia Kramer

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

With the goal of impacting patient quality life and outcomes, sensor science offers significant potential to revolutionize healthcare by providing advances in detection molecular biomarkers for personalized clinical technologies. The community has achieved technical advancements that can impact diagnostics, health monitoring, disease treatment; however, many innovations remain confined laboratory, failing bridge translational gap between research real-world applications. This perspective presents a new direction community, where development centers on needs experiences primary beneficiaries: patients. We provide guidelines resources researchers engage with patients early continuously throughout process inform specifications better align technologies needs, improving their adoption impact. also present examples implementing patient-centered approach planning engagement research. In design impactful sensors patients, must expand focus beyond embrace approach, which will likely lead opportunities collaboration evolution community.

Язык: Английский

Процитировано

0
A programmable magnetic digital microfluidic platform integrated with electrochemical detection system DOI Creative Commons
Yong Zhao,

Shuyue Jiang,

Gaozhe Cai

и другие.

Microsystems & Nanoengineering, Год журнала: 2025, Номер 11(1)

Опубликована: Май 12, 2025

Digital microfluidic (DMF) technology is widely used in bioanalysis and chemical reactions due to its accuracy flexibility manipulating droplets. However, most DMF systems usually rely on complex electrode fabrication high driving voltages. Sensor integration also quite rare. In this study, a programmable magnetic digital (PMDMF) platform integrated with electrochemical detection system was proposed. It enables non-contact, flexible droplet manipulation without processes voltages, meeting the requirements of automated detection. The includes control system, chip, an system. consists microcoil array circuit board, N52 permanent magnet, Arduino module. magnets generate localized fields drive movement, while module for precise manipulation. maximum average velocity about 3.9 cm/s. chip fabricated using 3D printing superhydrophobic surface by spray coating. MoS2@CeO2/PVA working electrode, Ag/AgCl reference carbon counter electrode. To evaluate practical value platform, glucose sweat automatically accurately detected. proposed has wide linear range (0.01-0.25 mM), lower LOD (6.5 μM), superior sensitivity (7833.54 μA·mM-1·cm-2), excellent recovery rate (88.1-113.5%). extensive potential future application medical diagnostics point-of-care testing.

Язык: Английский

Процитировано

0
CRISPR/Cas-powered nucleic acid amplification and amplification-free biosensors for public safety detection: Principles, advances and prospects DOI
Bo Yi,

Baoqing Zhou,

Donggen Zhou

и другие.

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108609 - 108609

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
CRISPR Digital Sensing: From Micronano-Collaborative Chip to Biomolecular Detection DOI
Xinyi Wang, Longjie Zhong, Wenbin Zhang

и другие.

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Май 24, 2025

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) sensing technology proved to be valuable during the COVID-19 pandemic through its sensitivity, specificity, robustness, and versatility. However, issues such as overreliance on amplification, susceptibility false positives, lack of quantification strategies, complex operation procedures have hindered broader application in bioanalysis clinical diagnostics. collision between micronano-collaborative chips CRISPR has effectively addressed these bottlenecks, offering innovative solutions for diagnosis treatment. Unlike conventional micronano chips, digital enhance CRISPR's response trace amounts target molecules by leveraging highly controllable local environments compartmentalized microreactors. This advancement improves detection efficiency revolutionizes traditional vitro bioanalytical processes. First, working principles, fabrication techniques, performance metrics CRISPR-based droplet microfluidics microarray are examined. Then, applications bioassays reviewed, emphasizing their importance advancing systems gene editing. Finally, prospects explored, particularly potential body surface biomonitoring development opportunities biomedical field.

Язык: Английский

Процитировано

0