Entrapment in Hydril Gels: Hydro-Ionic Liquid Polymer Gels for Enzyme Immobilization DOI

José Ángel Pérez Tomás,

Rebekah Brucato,

Preston Griffin

и другие.

Опубликована: Янв. 1, 2023

Enzyme entrapment in hydro-ionic liquid gels (HydrIL gels) was explored as an enzyme immobilization strategy. The phase of the a mixture buffer and tributyl methyl phosphonium alkanesulfonate [P4441][RSO3] ionic liquids. modifies creating more “organic” hydrophobic microenvironment around enzyme. In esterification secondary alcohol by Candida antarctica Lipase B, 20-fold enhancement final substrate conversion achieved using HydrIL gel compared to standard poly(acrylamide) hydrogel. Co-polymerization matrix with acrylate monomers led further increases reaction rate. A CalB reused over 10 cycles, achieving consistent performance from fifth cycle. IL leaching became undetectable third

Язык: Английский

Entrapment in Hydril Gels: Hydro-Ionic Liquid Polymer Gels for Enzyme Immobilization DOI

José Ángel Pérez Tomás,

Rebekah Brucato,

Preston Griffin

и другие.

Опубликована: Янв. 1, 2023

Enzyme entrapment in hydro-ionic liquid gels (HydrIL gels) was explored as an enzyme immobilization strategy. The phase of the a mixture buffer and tributyl methyl phosphonium alkanesulfonate [P4441][RSO3] ionic liquids. modifies creating more “organic” hydrophobic microenvironment around enzyme. In esterification secondary alcohol by Candida antarctica Lipase B, 20-fold enhancement final substrate conversion achieved using HydrIL gel compared to standard poly(acrylamide) hydrogel. Co-polymerization matrix with acrylate monomers led further increases reaction rate. A CalB reused over 10 cycles, achieving consistent performance from fifth cycle. IL leaching became undetectable third

Язык: Английский

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