Bistable Structures for Advanced Functional Systems

Advanced Functional Materials, Год журнала: 2021, Номер 31(45)

Опубликована: Авг. 6, 2021

Abstract Bistable mechanical systems having two local minima of potential energy can rest in either the stable equilibrium states absence external loadings. A snap‐through action may occur under suitable stimuli and/or loading, during which such exhibit distinct properties from linear structures. Such kinds structures have been widely exploited for designing advanced functional a variety applications. Here, advances bistable are summarized novel systems, including actuators, harvesters, microelectromechanical (MEMS), robotics, absorbers, and programmable devices as well metamaterials. The controllable motions highlighted nonlinear bistability/multistability. Finally, major principles, structures, pros cons, future research directions along with its challenges discussed.

Язык: Английский

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Unraveling the interaction between doxorubicin and DNA origami nanostructures for customizable chemotherapeutic drug release

Nucleic Acids Research, Год журнала: 2021, Номер 49(6), С. 3048 - 3062

Опубликована: Фев. 4, 2021

Abstract Doxorubicin (DOX) is a common drug in cancer chemotherapy, and its high DNA-binding affinity can be harnessed preparing DOX-loaded DNA nanostructures for targeted delivery therapeutics. Although DOX has been widely studied, the existing literature of DNA-carriers remains limited incoherent. Here, based on an in-depth spectroscopic analysis, we characterize optimize loading into different 2D 3D scaffolded origami (DONs). In our experimental conditions, all DONs show similar binding capacities (one molecule per two to three base pairs), equilibrium reached within seconds, remarkably faster than previously acknowledged. To release profiles, DON degradation from complexes upon DNase I digestion was studied. For employed DONs, relative doses (DOX molecules released unit time) may vary by orders magnitude depending superstructure. addition, identify aggregation mechanisms spectral changes linked pH, magnesium, concentration. These features have largely ignored experimenting with nanostructures, but are probably major sources incoherence results so far. Therefore, believe this work act as guide tailoring profiles developing better systems DNA-carriers.

Язык: Английский

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DNA nanostructure-based nucleic acid probes: construction and biological applications

Chemical Science, Год журнала: 2021, Номер 12(22), С. 7602 - 7622

Опубликована: Янв. 1, 2021

In recent years, DNA has been widely noted as a kind of material that can be used to construct building blocks for biosensing, in vivo imaging, drug development, and disease therapy because its advantages good biocompatibility programmable properties. However, traditional DNA-based sensing processes are mostly achieved by random diffusion free probes, which were restricted limited dynamics relatively low efficiency. Moreover, the application biosystems, single-stranded probes face challenges such being difficult internalize into cells easily decomposed cellular microenvironment. To overcome above limitations, nanostructure-based have attracted intense attention. This probe showed series compared conventional ones, including increased biostability, enhanced cell internalization efficiency, accelerated reaction rate, amplified signal output, thus improved vitro applications. Therefore, reviewing summarizing important roles nanostructures improving biosensor design is very necessary development nanotechnology applications biology pharmacology. this perspective, reviewed summarized from several aspects: classification according dimensions (one, two, three-dimensional nanostructures), common connection modes between nucleic acid nanostructures, most self-assembled imaging analysis, assembly, capture, theranostics. Finally, prospects future also discussed.

Язык: Английский

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Recent advances in DNA walker machines and their applications coupled with signal amplification strategies: A critical review

Analytica Chimica Acta, Год журнала: 2021, Номер 1171, С. 338523 - 338523

Опубликована: Апрель 17, 2021

Язык: Английский

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Increasingly Intelligent Micromachines

Annual Review of Control Robotics and Autonomous Systems, Год журнала: 2022, Номер 5(1), С. 279 - 310

Опубликована: Янв. 5, 2022

Intelligent micromachines, with dimensions ranging from a few millimeters down to hundreds of nanometers, are miniature systems capable performing specific tasks autonomously at small scales. Enhancing the intelligence micromachines tackle uncertainty and variability in complex microenvironments has applications minimally invasive medicine, bioengineering, water cleaning, analytical chemistry, more. Over past decade, significant progress been made construction intelligent evolving simple soft, compound, reconfigurable, encodable, multifunctional, integrated as well individual multiagent, multiscale, hierarchical, self-organizing, swarm micromachines. The field leverages two important trends robotics research—the miniaturization intelligentization machines—but compelling combination these features yet be realized. core technologies required make such tiny machines include information media, transduction, processing, exchange, energy supply, but embedding all functions into system micro- or nanoscale is challenging. This article offers comprehensive introduction state-of-the-art used create for provides insight next-generation that can adapt diverse scenarios use emerging fields.

Язык: Английский

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A DNA Molecular Robot that Autonomously Walks on the Cell Membrane to Drive Cell Motility

Angewandte Chemie International Edition, Год журнала: 2021, Номер 60(50), С. 26087 - 26095

Опубликована: Сен. 7, 2021

Abstract Synthetic molecular robots can execute sophisticated tasks at nanometer resolution. However, a robot capable of controlling cellular behavior remains unexplored. Herein, we report self‐propelled DNA operating on the cell membrane to control migration cell. Driven by DNAzyme catalytic activity, could autonomously and stepwise move membrane‐floating cell‐surface receptors in stochastic manner simultaneously trigger receptor‐dimerization activate downstream signaling for motility. The membrane‐associated continuous motion operation allowed ultrasensitive regulation MET/AKT cytoskeleton remodeling enhance migration. Finally, designed distinct conditional orthogonally manipulate coculture mixed populations. We have developed novel strategy engineer cell‐driving robot, representing promising avenue precise manipulation with nanoscale

Язык: Английский

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Dynamic Reconfigurable DNA Nanostructures, Networks and Materials

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(18)

Опубликована: Янв. 18, 2023

Abstract DNA nanotechnology relies on the structural and functional information encoded in nucleic acids. Specifically, sequence‐guided reconfiguration of acids by auxiliary triggers provides a means to develop switches, machines stimuli‐responsive materials. The present Review addresses recent advances construction applications dynamic reconfigurable nanostructures, networks Dynamic transformations proceeding within engineered origami frames or between tiles, triggered scaled supramolecular structures are addressed. use frameworks assemble chiroplasmonic optical devices operate switchable chemical processes discussed. Also, operation is addressed, design “smart” all‐DNA materials their introduced. Future perspectives nanostructures presented.

Язык: Английский

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Signal Amplification in Electrochemical DNA Biosensors Using Target-Capturing DNA Origami Tiles

ACS Sensors, Год журнала: 2023, Номер 8(4), С. 1471 - 1480

Опубликована: Март 13, 2023

Electrochemical DNA (e-DNA) biosensors are feasible tools for disease monitoring, with their ability to translate hybridization events between a desired nucleic acid target and functionalized transducer, into recordable electrical signals. Such an approach provides powerful method of sample analysis, strong potential generate rapid time result in response low analyte concentrations. Here, we report strategy the amplification electrochemical signals associated hybridization, by harnessing programmability origami construct sandwich assay boost charge transfer resistance (RCT) detection. This allowed improvement sensor limit detection two orders magnitude compared conventional label-free e-DNA biosensor design linearity concentrations 10 pM 1 nM without requirement probe labeling or enzymatic support. Additionally, this proved capable achieving high degree strand selectivity challenging DNA-rich environment. serves as practical addressing strict sensitivity requirements necessary low-cost point-of-care device.

Язык: Английский

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Reconfigurable pH-Responsive DNA Origami Lattices

ACS Nano, Год журнала: 2023, Номер 17(11), С. 11014 - 11022

Опубликована: Май 31, 2023

DNA nanotechnology enables straightforward fabrication of user-defined and nanometer-precise templates for a cornucopia different uses. To date, most these assemblies have been static, but dynamic structures are increasingly coming into view. The programmability not only allows encoding the object shape also it may be equally used in defining mechanism action type stimuli-responsiveness structures. However, "robotic" features nanostructures usually demonstrated small, discrete, device-like objects rather than collectively behaving higher-order systems. Here, we show how large-scale, two-dimensional (2D) pH-responsive origami-based lattice can assembled two configurations ("open" "closed" states) on mica substrate further switched from one to other distinct state upon pH change surrounding solution. control over is achieved by equipping arms lattice-forming origami units with "pH-latches" that form Hoogsteen-type triplexes at low pH. In short, demonstrate electrostatic adhesion mobility surface both large formation (with help directed polymerization) conformational switching whole lattice. emphasize feasibility method, 2D gold nanoparticle lattices. We believe this work bridge large-scale systems stimuli-induced dynamicity.

Язык: Английский

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Mechanism of DNA origami folding elucidated by mesoscopic simulations

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 8, 2024

Abstract Many experimental and computational efforts have sought to understand DNA origami folding, but the time length scales of this process pose significant challenges. Here, we present a mesoscopic model that uses switchable force field capture behavior single- double-stranded motifs transitions between them, allowing us simulate folding up several kilobases in size. Brownian dynamics simulations small structures reveal hierarchical involving zipping into partially folded precursor followed by crystallization final structure. We elucidate effects various design choices on order kinetics. Larger are found exhibit heterogeneous staple incorporation kinetics frequent trapping metastable states, as opposed more accessible which first-order virtually defect-free folding. This opens an avenue better nanostructures for improved yield performance.

Язык: Английский

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