bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 2, 2023
Abstract
Optogenetics
is
a
powerful
tool
for
spatiotemporal
control
of
gene
expression.
Several
light-inducible
regulators
have
been
developed
to
function
in
bacteria,
and
these
regulatory
circuits
ported
into
new
host
strains.
Here,
we
adapted
red
transcription
factor
Shewanella
oneidensis
.
This
circuit
based
on
the
iLight
optogenetic
system,
which
controls
expression
using
light.
Promoter
engineering
thermodynamic
model
were
used
adapt
this
system
achieve
differential
light
dark
conditions
within
S.
strain.
We
further
improved
by
adding
repressor
invert
genetic
activate
under
illumination.
The
inverted
was
extracellular
electron
transfer
(EET)
ability
use
both
blue
light-induced
simultaneously
demonstrated.
Our
work
expands
synthetic
biology
toolbox
,
could
facilitate
future
advances
applications
with
electrogenic
bacteria.
Current Opinion in Biotechnology,
Год журнала:
2024,
Номер
87, С. 103126 - 103126
Опубликована: Март 29, 2024
Molecular
optogenetics
utilizes
genetically
encoded,
light-responsive
protein
switches
to
control
the
function
of
molecular
processes.
Over
last
two
years,
there
have
been
notable
advances
in
development
novel
optogenetic
switches,
their
utilization
elucidating
intricate
signaling
pathways,
and
progress
toward
practical
applications
biotechnological
processes,
material
sciences,
therapeutic
applications.
In
this
review,
we
discuss
these
areas,
offer
insights
into
recent
developments,
contemplate
future
directions.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Окт. 14, 2022
Numerous
photoreceptors
and
genetic
circuits
emerged
over
the
past
two
decades
now
enable
light-dependent
i.e.,
optogenetic,
regulation
of
gene
expression
in
bacteria.
Prompted
by
light
cues
near-ultraviolet
to
near-infrared
region
electromagnetic
spectrum,
can
be
up-
or
downregulated
stringently,
reversibly,
non-invasively,
with
precision
space
time.
Here,
we
survey
underlying
principles,
available
options,
prominent
examples
optogenetically
regulated
While
transcription
initiation
elongation
remain
most
important
for
optogenetic
intervention,
other
processes
e.g.,
translation
downstream
events,
were
also
rendered
light-dependent.
The
control
bacterial
predominantly
employs
but
three
fundamental
strategies:
light-sensitive
two-component
systems,
oligomerization
reactions,
second-messenger
signaling.
Certain
moved
beyond
proof-of-principle
stood
test
practice.
They
unprecedented
applications
major
areas.
First,
underpins
novel
concepts
strategies
enhanced
yields
microbial
production
processes.
Second,
light-responsive
bacteria
stimulated
while
residing
within
bodies
animals,
thus
prompting
secretion
compounds
that
grant
health
benefits
animal
host.
Third,
optogenetics
allows
generation
precisely
structured,
biomaterials.
These
jointly
testify
maturity
approach
serve
as
blueprints
bound
inspire
template
innovative
use
cases
light-regulated
Researchers
pursuing
these
lines
choose
from
an
ever-growing,
versatile,
efficient
toolkit
circuits.
Nucleic Acids Research,
Год журнала:
2024,
Номер
52(13), С. 8003 - 8016
Опубликована: Июнь 11, 2024
Abstract
Optogenetics’
advancement
has
made
light
induction
attractive
for
controlling
biological
processes
due
to
its
advantages
of
fine-tunability,
reversibility,
and
low
toxicity.
The
lactose
operon
system,
commonly
used
in
Escherichia
coli,
relies
on
the
binding
or
isopropyl
β-d-1-thiogalactopyranoside
(IPTG)
repressor
protein
LacI,
playing
a
pivotal
role
operon.
Here,
we
harnessed
light-responsive
light-oxygen-voltage
2
(LOV2)
domain
from
Avena
sativa
phototropin
1
as
tool
control
engineered
LacI
into
two
variants,
OptoLacIL
OptoLacID.
These
variants
exhibit
direct
responsiveness
darkness,
respectively,
eliminating
need
IPTG.
Building
upon
OptoLacI,
constructed
light-controlled
E.
coli
gene
expression
systems,
OptoE.coliLight
system
OptoE.coliDark
system.
systems
enable
bifunctional
regulation
through
manipulation
show
superior
controllability
compared
IPTG-induced
systems.
We
applied
production
metabolic
flux
control.
Protein
levels
are
comparable
those
induced
by
Notably,
titers
dark-induced
1,3-propanediol
(1,3-PDO)
ergothioneine
exceeded
110%
60%
IPTG,
respectively.
development
OptoLacI
will
contribute
field
optogenetic
engineering,
holding
substantial
potential
applications
across
various
fields.
Microbial Cell Factories,
Год журнала:
2025,
Номер
24(1)
Опубликована: Фев. 16, 2025
The
commercial
growth
factors
(GFs)
and
serum
proteins
(SPs)
contribute
to
the
high
cost
associated
with
serum-free
media
for
cultivated
meat
production.
Producing
recombinant
GFs
SPs
in
scale
from
microbial
cell
factories
can
reduce
of
culture
media.
Escherichia
coli
is
a
frequently
employed
host
expression
SPs.
This
review
explores
critical
strategies
reduction
production,
focusing
on
yield
enhancement,
product
improvement,
purification
innovation,
process
innovation.
Firstly,
discusses
use
fusion
tags
increase
solubility
&
SPs,
highlighting
various
studies
that
have
successfully
these
enhancement.
We
then
explore
how
tagging
streamline
economize
process,
further
reducing
production
costs.
Additionally,
we
address
challenge
low
half-life
propose
potential
enhance
their
stability.
Furthermore,
improvements
E.
chassis
engineering
are
also
described,
an
emphasis
key
areas
improve
identify
minimization.
Finally,
discuss
bioprocessing
which
facilitate
easier
scale-up,
yield,
titer,
productivity,
ultimately
lower
long-term
It
crucial
recognize
not
all
suggested
approaches
be
applied
simultaneously,
as
relevance
varies
different
However,
integrating
multiple
anticipated
cumulative
effect,
significantly
collective
effort
expected
substantially
decrease
price
meat,
contributing
broader
goal
developing
sustainable
affordable
meat.
Annals of the New York Academy of Sciences,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 5, 2025
Abstract
The
human
gut
microbiome
is
a
complex
ecosystem
that
plays
vital
role
in
maintaining
health
and
contributing
to
the
pathogenesis
of
various
diseases.
This
review
proposes
transformative
approach
involves
engineering
artificial
microbial
consortia—precisely
designed
communities
microorganisms—for
personalized
modulation
targeted
therapeutic
interventions.
By
integrating
synthetic
biology,
systems
advanced
culturing
techniques,
tailored
consortia
can
be
developed
perform
specific
functions
within
gut,
including
production
molecules,
immune
responses,
competition
against
pathogenic
bacteria.
In
vitro
vivo
studies
indicate
these
engineered
effectively
restore
balance
enhance
host
resilience.
holds
immense
potential
revolutionize
healthcare
by
addressing
root
causes
diseases
such
as
metabolic
disorders,
inflammatory
conditions,
gastrointestinal
infections
through
precise
manipulation
microbiome.
Future
research
should
focus
on
rigorous
clinical
trials
evaluate
safety,
efficacy,
long‐term
impacts
diverse
populations,
paving
way
for
innovative
therapies
promote
overall
well‐being.
