Gallic
acid
(GA),
widely
used
in
food
and
medicine
production
industries.
Herein,
a
kind
of
newly
synthesized
cobalt-nitrogen
co-doped
carbon
dots
(Co,N-CDs)
with
dual
functional
properties
oxidase-like
activity
photoluminescence
property
could
oxidize
the
3,3',5,5'-tetramethylbenzidine
(TMB)
to
oxTMB
new
absorption
peak
at
653
nm.
In
addition,
it
was
found
that
performed
fluorescence-quenching
effect
on
Co,N-CDs
440
nm
attributed
FRET
(Fluorescence
resonance
energy
transfer).
Reductive
GA
reduce
TMB,
weaken
blue
color,
restore
fluorescence
Co,N-CDs.
Thus,
colorimetric
fluorometric
method
for
dual-mode
detection
established.
The
synthesis
dual-signal
probe
completed
single
environmental
precursor
just
one
step.
Simultaneously,
reduced
limits,
broadened
linear
range
improved
anti-interference
ability.
This
exhibited
satisfactory
application
prospect
actual
tea
beverages.
Case Studies in Chemical and Environmental Engineering,
Год журнала:
2024,
Номер
10, С. 100776 - 100776
Опубликована: Май 28, 2024
The
growing
global
population
challenges
the
food
industry
to
ensure
it
remains
safe
for
consumption.
Food
waste
caused
by
imbalances
between
production
and
handling
creates
significant
economic,
environmental,
social
risks.
However,
packaging
technology
innovation
has
emerged
as
a
powerful
tool,
offering
solution
extending
shelf
life
maintaining
product
quality.
Consumers'
increasing
interest
in
eco-friendly
driven
research
into
renewable
materials
low-toxicity
bioactive
components.
Gallic
acid,
promising
phenolic
compound,
holds
potential
preservation
sustainable
development.
Its
antibacterial
antifungal
properties
against
resistant
microbes
biofilm
formation,
its
ability
prevent
lipid
protein
oxidation,
color
indicator
monitoring
freshness
all
contribute
preservation.
incorporation
edible
polymers
offers
strategy
innovative
packaging.
Depending
on
dosage
material
composition,
loading
gallic
acid
within
polymer
matrix
can
improve
properties,
including
UV
barrier,
mechanical
strength,
water
vapor
oxygen
permeability.
This
article
reviews
of
developing
biodegradable,
active,
intelligent,
Future
directions
include
evaluating
acid's
toxicity,
dispersion
matrix,
pro-oxidative
activity,
compatibility
with
biodegradable
(especially
those
derived
from
agricultural
by-products),
migration
products,
stability
under
different
environmental
conditions.
sustainability
perspective
(environment,
social)
is
also
discussed.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Фев. 9, 2024
Abstract
Bioengineers
increasingly
rely
on
ligand-inducible
transcription
regulators
for
chemical-responsive
control
of
gene
expression,
yet
the
number
available
is
limited.
Novel
can
be
mined
from
genomes,
but
an
inadequate
understanding
their
DNA
specificity
complicates
genetic
design.
Here
we
present
Snowprint,
a
simple
powerful
bioinformatic
tool
predicting
regulator:operator
interactions.
Benchmarking
results
demonstrate
that
Snowprint
predictions
are
significantly
similar
>45%
experimentally
validated
pairs
organisms
across
nine
phyla
and
span
five
distinct
structural
families.
We
then
use
to
design
promoters
33
previously
uncharacterized
sourced
diverse
phylogenies,
which
28
shown
influence
expression
24
produce
>20-fold
dynamic
range.
A
panel
newly
repurposed
screened
response
biomanufacturing-relevant
compounds,
yielding
new
sensors
polyketide
(olivetolic
acid),
terpene
(geraniol),
steroid
(ursodiol),
alkaloid
(tetrahydropapaverine)
with
induction
ratios
up
10.7-fold.
represents
unique,
protein-agnostic
greatly
facilitates
discovery
transcriptional
bioengineering
applications.
web-accessible
version
at
https://snowprint.groov.bio
.
Biotechnology and Bioengineering,
Год журнала:
2024,
Номер
121(11), С. 3527 - 3536
Опубликована: Июль 30, 2024
Abstract
Gallic
acid
(GA)
and
β‐glucogallin
(BGG)
are
natural
products
with
diverse
uses
in
pharmaceutical,
food,
chemical
cosmetic
industries.
They
valued
for
their
wide‐ranging
properties
such
as
antioxidant,
antibacterial,
antidiabetic,
anticancer
properties.
Despite
significant
importance,
microbial
production
of
GA
BGG
faces
challenges
limited
titers
yields,
along
the
incomplete
understanding
biosynthesis
pathways
microorganisms.
To
address
these
challenges,
we
developed
a
recombinant
Escherichia
coli
strain
capable
efficiently
producing
GA.
Our
approach
involved
screening
efficient
pathway
enzymes,
integrating
biosynthetic
genes
into
genome
while
balancing
carbon
flux
via
adjusting
expression
levels,
strengthening
shikimate
to
remove
bottlenecks.
The
resultant
achieved
impressive
results,
51.57
g/L
yield
0.45
g/g
glucose
productivity
1.07
g/L/h.
Furthermore,
extended
this
platform
biosynthesize
by
1‐
O
‐glucosyltransferase,
leading
de
novo
92.42
mg/L
BGG.
This
work
establishes
an
chassis
at
industrial
level
provides
generating
derivatives.
Abstract
Background:
Lignin
holds
great
potential
as
an
abundant
and
sustainable
source
of
aromatic
compounds,
offering
a
viable
alternative
to
fossil-based
resources
for
producing
chemicals
materials.
Biological
upgrading
lignin-derived
aromatics
can
lead
more
comprehensive
lignocellulose
utilization,
thereby
enhancing
the
overall
feasibility
production.
However,
exploring
broader
range
microbial
hosts,
pathways,
enzymes
is
crucial
developing
efficient
conversion
processes.
In
particular,
improving
S-lignin-related
aromatics,
such
syringate,
remains
key
area
future
research.
Results:
this
study,
we
aimed
investigate
syringate
in
Acinetobacter
baylyi
ADP1
by
exploiting
its
native
vanillate
demethylase,
VanAB.
We
discovered
that
wild-type
strain
efficiently
O-demethylate
3-O-methylgallate
(3MGA)
then
gallate,
revealing
previously
unknown
activity
VanAB
A.
ADP1.
Conversion
dynamics
in
vitro
characterization
showed
prefers
substrate
over
3MGA.
Overexpression
vanAB
resulted
simultaneous
3MGA,
but
negatively
impacted
growth,
potentially
due
toxic
side
product
formaldehyde
redox
imbalance
caused
high
NADH
consumption
O-demethylation
reactions.
Native
expression
3MGA
accumulation
if
was
available.
took
advantage
constructing
with
heterologous
galA,
gallate
dioxygenase
from
Pseudomonas
putida
KT2440,
demonstrated
into
2-pyrone-4,6,-dicarboxylate
(PDC),
precursor
high-quality
polyesters.
Conclusions:
By
adjusting
level
vanAB,
be
directed
either
or
which
could
further
converted
PDC
through
galA.
Our
results
highlight
versatility
lignin
valorisation.
