BIOspektrum, Год журнала: 2025, Номер 31(3), С. 334 - 334
Опубликована: Май 1, 2025
Biotechnology Advances, Год журнала: 2024, Номер 77, С. 108464 - 108464
Опубликована: Окт. 9, 2024
Biotechnology has been built on the foundation of a small handful well characterized and well-engineered organisms. Recent years have seen breakout performer gain attention as new entrant into bioengineering toolbox: Vibrio natriegens. This review covers recent research efforts making V. natriegens biotechnology platform, using large language model (LLM) knowledge graph to expedite literature survey process. Scientists made advancements in pertaining fundamental metabolic characteristics natriegens, development characterization synthetic biology tools, systems analysis modeling, bioproduction engineering, microbial ecology. Each these subcategories relevance future for applications. In this review, we cover offer context impact they may field, highlighting benefits drawbacks organism. From examining research, it appears that is precipice becoming platform bacterium biotechnology.
Язык: Английский
Процитировано
3
Bioprocess and Biosystems Engineering, Год журнала: 2025, Номер unknown
Опубликована: Апрель 18, 2025
Abstract Vibrio natriegens is a fast-growing microbial workhorse with high potential for biotechnological applications. However, handling the bacterium in batch processes challenging due to its overflow metabolism and mixed acid formation under microaerobic conditions. For early process development, technologies enabling small-scale fed-batch cultivation of V. Vmax are needed. In this study, cultivations 96-well microtiter plates were successfully online-monitored first time µTOM device. Using oxygen transfer rate, scale up membrane-based shake flasks was performed. The efficiently minimized by choosing suitable feed rates, prevented. A glucose soft sensor using rate provided accurate estimates consumption throughout fermentation, eliminating need offline sampling. Analyzing impact inducer IPTG on recombinant production enzyme inulosucrase revealed concentration-dependent effects processes. contrast, operating mode resulted activity even without induction. Overall, an titer 80 U/mL achieved. conclusion, advantages supported have been demonstrated development Vmax.
Язык: Английский
Процитировано
0
BIOspektrum, Год журнала: 2025, Номер 31(3), С. 334 - 334
Опубликована: Май 1, 2025
Процитировано
0