Design and Synthesis of two novel carbazole-based fluorescent probes and their application in detecting Hg2+
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy,
Год журнала:
2025,
Номер
340, С. 126352 - 126352
Опубликована: Май 9, 2025
Язык: Английский
Benzimidazole-Derived B2 as a Fluorescent Probe for Bacterial Outer Membrane Vesicle (OMV) Labeling: Integrating DFT, Molecular Dynamics, Flow Cytometry, and Confocal Microscopy
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4682 - 4682
Опубликована: Май 14, 2025
Bacterial
outer
membrane
vesicles
(OMVs)
are
nanoscale
extracellular
structures
produced
by
Gram-negative
bacteria
that
critical
for
microbial
biology
and
host-pathogen
interactions
have
great
potential
in
biotechnological
applications.
Despite
the
availability
of
fluorescent
dyes
OMV
studies,
many
repurposed
from
eukaryotic
vesicle
research
not
explicitly
optimized
OMVs,
leading
to
challenges
achieving
consistent
labeling,
minimizing
background
noise,
preserving
integrity
during
analyses.
This
study
evaluates
B2,
a
benzimidazole-derived
fluorophore,
labeling
advanced
techniques
like
flow
cytometry
confocal
microscopy.
OMVs
were
isolated
Escherichia
coli
strains
BL21
O157,
their
was
confirmed
using
transmission
electron
microscopy
(TEM).
B2
staining
protocols
fluorescence
analyses
revealed
specific
with
membranes,
reducing
aggregation
enhancing
signal
uniformity.
Flow
indicated
near-complete
efficiency
(98-100%)
minimal
interference.
Confocal
further
validated
B2's
effectiveness,
showing
evident
internalization
into
epithelial
HT-29
cells
compatibility
other
fluorophores.
Density
functional
theory
(DFT)
calculations,
including
Fukui
function
analysis,
identified
key
electrophilic
nucleophilic
regions
facilitate
hydrogen
bonding
polar
components.
Non-covalent
interaction
(NCI)
analysis
pronounced
intramolecular
along
discrete
weak
van
der
Waals
interactions.
Molecular
dynamics
simulations
suggest
exhibits
an
affinity
both
hydrophobic
core
lipid
bilayer
oligosaccharide
region
LPS
layer,
which
collectively
ensures
sustained
retention
dye.
The
findings
presented
this
position
as
valuable
fluorophore
research.
Язык: Английский
A Smart Triple-Signal Fluorescent Probe for Real-Time Differential Imaging of HClO, H2O2, and Their Mixture in Diabetic Models
Chemical & Biomedical Imaging,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 20, 2025
Язык: Английский
A Highly Selective and Sensitive Fluorescent Probe With a Large Stokes Shift for Near-Infrared Visualization of Endogenous and Exogenous Biothiols in Living Cells
Frontiers in Bioscience-Landmark,
Год журнала:
2025,
Номер
30(5)
Опубликована: Май 23, 2025
Background:
Fluorescent
probes
have
become
a
powerful
tool
for
monitoring
biothiol
concentrations,
aiding
in
disease
diagnosis
and
treatment
while
also
facilitating
the
exploration
of
fundamental
biological
processes.
However,
are
limited
by
short
fluorescence
emission
wavelength
small
Stokes
shift,
which
makes
them
susceptible
to
background
interference
significant
self-absorption.
To
overcome
these
limitations
achieve
high-fidelity
biothiols
detection
complex
systems,
this
study
focuses
on
developing
near-infrared
fluorescent
probe
with
an
extended
shift.
Methods:
(E)-4-(5-(2-(4-(dicyanomethylene)-4H-chromen-2-yl)vinyl)thiophen-2-yl)phenyl
2,4-dinitrobenzenesulfonate
(DCMOS-N),
(NIR)
featuring
large
was
designed
synthesized
detection.
The
optical
properties
DCMOS-N
were
evaluated
using
ultraviolet-visible
(UV-Vis)
spectroscopy.
Additionally,
its
imaging
capabilities
detecting
living
cells
assessed
through
confocal
microscopy.
Results:
Fluorescence
spectral
analysis
confirmed
that
exhibits
high
selectivity
strong
anti-interference
Moreover,
intensity
increases
upon
addition
biothiols.
Notably,
linear
correlation
observed
across
concentration
range
0
100
μmol/L
(R2
=
0.9944
glutathione
(GSH),
0.9942
cysteine
(Cys),
0.9946
homocysteine
(Hcy)),
enabling
quantitative
concentrations
systems.
limits
GSH,
Cys,
Hcy
determined
as
0.142
μmol/L,
0.129
0.143
respectively.
Importantly,
practical
application
validated,
demonstrating
capability
detect
both
endogenous
exogenous
HeLa
cells.
Conclusion:
An
NIR
probe,
DCMOS-N,
developed
effectively
utilized
monitor
successful
design
presents
potential
serves
innovative
platform
targeted
at
Язык: Английский
Screening Anti-Parkinson’s Disease Drugs in Living Mouse Brains via a Peroxynitrite-Activated Fluorescent Probe
Chemical & Biomedical Imaging,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 20, 2024
Screening
anti-Parkinson's
disease
(PD)
drugs
at
in
vivo
brain
level
is
imperative
for
managing
PD
yet
currently
remains
unaccomplished.
Peroxynitrite
(ONOO–)
has
been
implicated
progression.
Thus,
developing
ONOO–-based
imaging
tools
anti-PD
drug
screening
holds
promise
early
prognosis
and
treatment
of
PD.
Consequently,
a
near-infrared
(NIR)
fluorescence
probe,
BOB-Cl-PN,
with
high
specificity,
good
sensitivity
(LOD
=
24
nM),
rapid
response
(<60
s),
was
devised
to
investigate
ONOO–
relationships.
Utilizing
NIR
imaging,
BOB-Cl-PN
effectively
monitored
fluctuations
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
(MPTP)-induced
cell
models,
establishing
cellular
throughput
(cHTS)
system
drugs.
In
live
animal
BOB-Cl-PN's
ability
penetrate
the
blood–brain
barrier
enabled
flux
mouse
brains.
Moreover,
served
as
an
contrast
potential
traditional
Chinese
medicines
therapy,
identifying
fisetin
having
best
therapeutic
index
among
10
medicines.
This
study
constructs
sensitive,
efficient
monitoring
dynamics
brains
provides
valuable
platform
Язык: Английский