Activity-based NIR fluorescent probes based on the versatile hemicyanine scaffold: design strategy, biomedical applications, and outlook

Chemical Society Reviews, Год журнала: 2022, Номер 51(5), С. 1795 - 1835

Опубликована: Янв. 1, 2022

The discovery of a near-infrared (NIR, 650-900 nm) fluorescent chromophore hemicyanine dye with high structural tailorability is great significance in the field detection, bioimaging, and medical therapeutic applications. It exhibits many outstanding advantages including absorption emission NIR region, tunable spectral properties, photostability as well large Stokes shift. These properties are superior to those conventional fluorogens, such coumarin, fluorescein, naphthalimides, rhodamine, cyanine. Researchers have made remarkable progress developing activity-based multifunctional probes based on skeletons for monitoring vital biomolecules living systems through output fluorescence/photoacoustic signals, integration diagnosis treatment diseases using chemotherapy or photothermal/photodynamic therapy combination therapy. achievements prompted researchers develop more smart fluorogen template. In this review, we begin by describing brief history dyes, synthetic approaches, design strategies functional probes. Then, selected hemicyanine-based that can detect ions, small biomolecules, overexpressed enzymes diagnostic reagents systematically highlighted. Finally, potential drawbacks outlook future investigation clinical medicine transformation activatable also discussed.

Язык: Английский

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Raman Spectroscopy for Chemical Biology Research

Journal of the American Chemical Society, Год журнала: 2022, Номер 144(43), С. 19651 - 19667

Опубликована: Окт. 10, 2022

In chemical biology research, various fluorescent probes have been developed and used to visualize target proteins or molecules in living cells tissues, yet there are limitations this technology, such as the limited number of colors that can be detected simultaneously. Recently, Raman spectroscopy has applied overcome limitations. detects molecular vibrations reflecting structures conditions a sample was originally directly responses endogenous molecules. However, our initial research develop "Raman tags" opens new avenue for application biology. Perspective, we first introduce label-free imaging biomolecules, illustrating biological applications spectroscopy. Next, highlight small using tags research. Finally, discuss development potential probes, which represent next-generation

Язык: Английский

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Activity-Based Fluorescence Diagnostics for Cancer

Chemical Reviews, Год журнала: 2024, Номер 124(7), С. 4021 - 4078

Опубликована: Март 22, 2024

Fluorescence imaging is one of the most promising approaches to achieve intraoperative assessment tumor/normal tissue margins during cancer surgery. This critical improve patients' prognosis, and therefore various molecular fluorescence probes have been developed for identification lesions Among them, "activatable" that react with cancer-specific biomarker enzymes generate signals great potential high-contrast due their low background high signal amplification by enzymatic turnover. Over past two decades, activatable employing control mechanisms worldwide this purpose. Furthermore, new activities specific types cancers identified, enabling visualization sensitivity specificity. Review focuses on recent advances in design, function characteristics target also discusses future prospects field activity-based diagnostics cancer.

Язык: Английский

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A highly selective and sensitive chemiluminescent probe for leucine aminopeptidase detection in vitro, in vivo and in human liver cancer tissue

Chemical Science, Год журнала: 2022, Номер 13(8), С. 2324 - 2330

Опубликована: Янв. 1, 2022

Leucine aminopeptidase (LAP) is involved in tumor cell proliferation, invasion, and angiogenesis, a well-known marker. In recent years, chemiluminescence has been widely used the field of biological imaging, due to it resulting high sensitivity excellent signal-to-noise ratio. Here, we report design, synthesis, evaluation first LAP-activated chemiluminescent probe for LAP detection imaging. The initially had no but produced an extremely strong after release dioxetane intermediate presence LAP. selectivity over other proteases higher ratios than commercial fluorophores. Real-time imaging results indicated that was remarkably enhanced at mice site injected. Furthermore, this cancerous tissues patients obviously improved compared normal tissues. Taken together, study developed LAP-activable probe, which could be potentially protein detection, disease diagnosis, drug development.

Язык: Английский

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Super‐Resolution Vibrational Imaging Using Expansion Stimulated Raman Scattering Microscopy

Advanced Science, Год журнала: 2022, Номер 9(20)

Опубликована: Май 6, 2022

Abstract Stimulated Raman scattering (SRS) microscopy is an emerging technology that provides high chemical specificity for endogenous biomolecules and can circumvent common constraints of fluorescence including limited capabilities to probe small difficulty resolving many colors simultaneously. However, the resolution SRS remains governed by diffraction limit. To overcome this, a new technique called molecule anchorable gel‐enabled nanoscale Imaging Fluorescence stimulated (MAGNIFIERS) integrates with expansion (ExM) described. MAGNIFIERS offers chemical‐specific imaging sub‐50 nm has scalable multiplexity when combined multiplex probes fluorescent labels. used visualize features in label‐free manner CH vibration proteins, lipids, DNA broad range biological specimens, from mouse brain, liver, kidney human lung organoid. In addition, applied track protein synthesis aggregates using metabolic labeling metabolites. Finally, demonstrate 8‐color expanded brain section. Overall, valuable platform super‐resolution imaging, high‐resolution highly multiplexed thus bringing nanoscopy.

Язык: Английский

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Engineered Core–Shell Multifunctional Nano‐Tracer in Raman‐Silent Region with Highly Retained Affinity to Enhance Lateral Flow Immunoassays

Small, Год журнала: 2022, Номер 18(45)

Опубликована: Сен. 26, 2022

Abstract Stimulated surface‐enhanced Raman scattering (SERS) in combination with engineered nano‐tracer offers extraordinary potential lateral flow immunoassays (LFIAs). Nonetheless, the investigation execution of SERS‐LFIA is often compromised by intricacy and overlap fingerprint spectrum as well affinity‐interference to antibody. To circumvent these critical issues, an core–shell multifunctional (named APNPs) precise control size nano‐core (AuNPs) coating nano‐shell (Prussian blue nanomaterials) prepared for via a modified enlarging particle modification strategy. Importantly, this exhibits enhanced coupling efficiency, highly retained affinity, reinforced colloid stability, unique SERS signal (2156 cm −1 ) silent region (1800–2800 high signal‐to‐background ratio simultaneously, all which are beneficial enhancement analysis performance. With proof‐of‐concept demonstration detection ractopamine (RAC), dual‐pattern LFIA that synergizes both enlarged supported colorimetric/biological silence dual‐response (coined ECCRD assay) demonstrated integrating APNPs competitive‐type immunoreaction. This research may contribute rational design nano‐tracer, assay can be expanded wide applications environmental monitoring biomedical diagnosis.

Язык: Английский

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Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

Chemical Science, Год журнала: 2023, Номер 14(25), С. 6953 - 6962

Опубликована: Янв. 1, 2023

Multiplex technology is an important emerging field, in diagnostic sciences, that enables the simultaneous detection of several analytes a single sample. The light-emission spectrum chemiluminescent phenoxy-dioxetane luminophore can be accurately predicted by determining fluorescence-emission its corresponding benzoate species, which generated during chemiexcitation process. Based on this observation, we designed library dioxetane luminophores with multicolor emission wavelengths. Two have different spectra, but similar quantum yield properties, were selected from synthesized for duplex analysis. equipped two enzymatic substrates to generate turn-ON probes. This pair probes exhibited promising ability act as system activities physiological solution. In addition, also able simultaneously detect enzymes bacterial assay, using blue filter slit one enzyme and red other enzyme. As far know, first successful demonstration composed two-color phenoxy-1,2-dioxetane luminophores. We believe dioxetanes presented here will beneficial developing chemiluminescence multiplex analysis bioanalytes.

Язык: Английский

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Determination of Intracellular Esterase Activity Using Ratiometric Raman Sensing and Spectral Phasor Analysis

Analytical Chemistry, Год журнала: 2023, Номер 95(12), С. 5369 - 5376

Опубликована: Март 16, 2023

Carboxylesterases (CEs) are a class of enzymes that catalyze the hydrolysis esters in variety endogenous and exogenous molecules. CEs play an important role drug metabolism, onset progression disease, can be harnessed for prodrug activation strategies. As such, regulation is clinical pharmaceutical consideration. Here, we report first ratiometric sensor CE activity using Raman spectroscopy based on bisarylbutadiyne scaffold. The was shown to highly sensitive specific detection had low cellular cytotoxicity. In hepatocyte cells, esterase possible, result validated by multimodal imaging with standard viability stains used fluorescence microscopy within same cell population. addition, show localized ultraviolet damage mixed population possible stimulated scattering coupled spectral phasor analysis. This demonstrates practical advantages molecular weight sensors detected will have applications discovery biomedical research.

Язык: Английский

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Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons

Nature Methods, Год журнала: 2024, Номер 21(2), С. 342 - 352

Опубликована: Янв. 8, 2024

Язык: Английский

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Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 22, 2024

Abstract Optical imaging with photo-controllable probes has greatly advanced biological research. With superb chemical specificity of vibrational spectroscopy, stimulated Raman scattering (SRS) microscopy is particularly promising for super-multiplexed optical rich information. Functional SRS in response to light been recently demonstrated, but multiplexed reversible photocontrol remains unaccomplished. Here, we create a multiplexing palette photoswitchable polyynes 16 frequencies by coupling asymmetric diarylethene Carbow (Carbow-switch). Through optimization both electronic and Carbow-switch displays excellent photoswitching properties under visible control large frequency change signal enhancement. Reversible spatial-selective different organelles are demonstrated living cells. We further achieve photo-selective time-lapse organelle dynamics during oxidative stress protein phase separation. The development will open up new avenues study complex interactions cells high spatiotemporal precision capability.

Язык: Английский

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