European Robin Cryptochrome-4a Associates with Lipid Bilayers in an Ordered Manner, Fulfilling a Molecular-Level Condition for Magnetoreception DOI Creative Commons
Marta Majewska, Maja Hanić, Rabea Bartölke

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 23, 2024

Abstract Since the middle of 20th century, long-distance avian migration has been known to rely partly on geomagnetic field. However, underlying sensory mechanism is still not fully understood. Cryptochrome 4a (ErCry4a), found in European Robin ( Erithacus rubecula ), a night-migratory songbird suggested be magnetic molecule. It sensitive external fields via so-called radical-pair mechanism. ErCry4a primarily located outer segments double cone photoreceptor cells eye, which contain stacked and highly ordered membranes that could facilitate anisotropic attachment needed for compass sensing. Here, we investigate possible interactions with model membrane mimics lipid composition vertebrate by using experimental computational approaches. Experimental results show controlled physical state molecules (average area per lipid) leaflet bilayer. Furthermore, polarization modulation infrared reflection absorption spectroscopy allowed us determine conformation, motional freedom, average orientation α- helices membrane-associated state. Atomistic molecular dynamics studies supported results. A ∼1000 kcal mol −1 decrease interaction energy as result binding was determined compared cases where no protein occurred. At level, seems involve negatively charged carboxylate groups phosphoserine lipids C-terminal residues ErCry4a. Our study reveals potential direct discusses how this an essential step propagate signal further thus fulfill role magnetoreceptor.

Язык: Английский

European Robin Cryptochrome-4a Associates with Lipid Bilayers in an Ordered Manner, Fulfilling a Molecular-Level Condition for Magnetoreception DOI Creative Commons
Marta Majewska, Maja Hanić, Rabea Bartölke

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 23, 2024

Abstract Since the middle of 20th century, long-distance avian migration has been known to rely partly on geomagnetic field. However, underlying sensory mechanism is still not fully understood. Cryptochrome 4a (ErCry4a), found in European Robin ( Erithacus rubecula ), a night-migratory songbird suggested be magnetic molecule. It sensitive external fields via so-called radical-pair mechanism. ErCry4a primarily located outer segments double cone photoreceptor cells eye, which contain stacked and highly ordered membranes that could facilitate anisotropic attachment needed for compass sensing. Here, we investigate possible interactions with model membrane mimics lipid composition vertebrate by using experimental computational approaches. Experimental results show controlled physical state molecules (average area per lipid) leaflet bilayer. Furthermore, polarization modulation infrared reflection absorption spectroscopy allowed us determine conformation, motional freedom, average orientation α- helices membrane-associated state. Atomistic molecular dynamics studies supported results. A ∼1000 kcal mol −1 decrease interaction energy as result binding was determined compared cases where no protein occurred. At level, seems involve negatively charged carboxylate groups phosphoserine lipids C-terminal residues ErCry4a. Our study reveals potential direct discusses how this an essential step propagate signal further thus fulfill role magnetoreceptor.

Язык: Английский

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